고수 엑소좀의 Tyrosinase와 TRPs 발현 억제를 통한 피부 미백 증진 효과
Melanin Synthesis Gene Inhibition Effect through Inhibition of Tyrosinase and TRPs Expression of Coriandrum sativum-derived Exosome
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목적: 본 연구에서는 Coriandrum sativum (C. sativum) 유래 엑소좀을 이용하여 멜라닌 합성을 억제함으로써 피부 미백 효과를 알아보고자 하였다. 방법: 나노입자 추적 분석을 사용하여 C. sativum 유래 엑소좀(C. sativum exosome, CSE)의 특성을 조사한 결과, 평균 크기는 152.3 nm이고 농도는 3.0×1010 particles/mL인 것으로 나타났다. 세포 안전성을 평가하기 위해 B16F0 세포에 대한 세포독성 평가를 실시하였고, RT-PCR을 통해 멜라닌 합성 유전자의 발현 수준을 정량하는 방법을 사용하여 CSE의 피부 미백효과를 조사하였다. 결과: 1.5×108 particles/mL 미만의 CSE 농도가 세포 활동에 부정적인 영향을 미치지 않는 것으로 나타났다. CSE 처리는 tyrosinase, TRP-1와 TRP-2 등 멜라닌 합성에 관여하는 주요 유전자의 발현을 각각 75.6%, 89.1%, 88.7% 유의하게감소시켰다. 결론: CSE는 멜라닌 생성에 관여하는 핵심 유전자를 효과적으로 억제함으로써 피부 미백 촉진 기능성 화장품 원료로서 높은 잠재력을 보였다.
Abstract
Purpose: This study investigated the skin-whitening effects achieved through suppressing melanin synthesis using exosomes derived from Coriandrum sativum (C. sativum). Methods: We examined the characteristics of C. sativum exosomes (CSEs) using nanoparticle tracking analysis, which revealed an average size of 152.3 nm and a concentration of 3.0×1010 particles/mL. A cytotoxicity evaluation was conducted on B16F0 cells to assess cellular safety of CSEs. We investigated the skin-whitening effects of CSEs by quantifying melanin synthesis gene expression using RT-PCR. Results: CSE concentrations less than 1.5×108 particles/mL did not negatively affect cellular activity. Furthermore, CSE treatment significantly decreased the expression of key genes involved in melanin synthesis such as tyrosinase, TRP1, and TRP-2 by 75.6%, 89.1%, and 88.7%, respectively. Conclusion: CSEs hold promise as functional cosmetic ingredients that promote skin whitening by inhibiting key genes involved in melanin production.
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