인간 각질형성세포에서 국산 동결건조 로열젤리의 피부 건강 개선 효과
Skin Health Improving Effects of Korean Freeze-dried Royal Jelly in Human Keratinocytes
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목적: 본 연구는 국산 로열젤리의 피부건강에 도움을 주는 건강기능식품 원료로 개발하기 위하여 동결건조 로열젤리와 주요성분인10-Hydroxydecanoic acid (10-HDA) 피부 노화 억제효과를 확인하고자 하였다. 방법: 인간 각질형성세포(HaCaT)를 대상으로 국산 동결건조 로열젤리와 10-HDA 처리에 따른 피부 상처 모델에서의 세포 증식 및 이주 효능을 EZ-cytox kit assay를 이용하여 분석하였다. 또한 자외선 B 를 조사 후 HaCaT 세포에서 피부 노화에 관련된 matrix metalloproteinase (MMP)-1과 MMP-3의 생성량 변화는 enzyme-linked immunosorbent assay로 확인하였고 유전자 발현은 real-time polymerase chain reaction으로 분석하였다. 결과: 국산 동결건조 로열젤리와 10-HDA는 기계적으로 손상된 HaCaT 세포의 재생 촉진과 및 이주 능력을 향상시켰으며 특히동결건조 로열젤리 100 µg/mL 및 10-HDA 100 µM의 농도로 처리한 시험군은 양성 대조군으로 사용한 vitamin C와 유사한 것으로 확인되었다. 또한 자외선 B로 인해 손상된 HaCaT 세포에서 콜라겐 생성을 억제하여 피부노화를 유발하는 MMP-1과 MMP-3 생성 및 유전자 발현량을 유의적으로 감소시켰다. 결론: 본 연구 결과를 통하여 국산 동결건조 로열젤리와 로열젤리의 주요 성분인10-HDA의 피부 주름 생성을 억제 효능을 확인하였으며 피부 건강 기능성식품 원료로서 가능성을 제시하였다.
Abstract
Purpose: In this study, we aimed to examine the anti-skin aging effects of Korean freeze-dried royal jelly and its main ingredient, 10-Hydroxydecanoic acid (10-HDA), in order to promote Korean royal jelly as a health functional food ingredient for improving skin health. Methods: The effects of Korean freeze-dried royal jelly and 10-HDA were determined by examining the proliferation and migration of human keratinocytes (HaCaT cells) in a skin wound healing model by using EZ-cytox kit assay. The changes in production of matrix metalloproteinase (MMP)-1 and MMP-3, which are related to skin aging in HaCaT cells, after UV-B irradiation were analyzed using enzyme-linked immunosorbent assay. Real-time polymerase chain reaction was employed to analyze MMP-1 and MMP-3 gene expression. Results: Korean freeze-dried royal jelly and 10-HDA improved the regeneration and migration of mechanically damaged HaCaT cells. The test group treated with 100 µg/mL of Korean freeze-dried royal jelly and 100 µM of 10-HDA yielded similar results to those obtained for the positive control group treated with vitamin C. Furthermore, Korean freeze-dried royal jelly and 10-HDA significantly reduced the production and gene expression of MMP-1 and MMP-3, which are the key elements that induce skin aging by suppressing collagen generation in UVB-irradiated HaCaT cells. Conclusion: We examined the anti-wrinkle effects of Korean freeze-dried royal jelly and its main ingredient, 10-HDA. The results suggested the applicability of Korean royal jelly as a key functional health food ingredient for improving skin health.
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