충남지역 지하수에서 분리한 총대장균군 양성시료 중 수인성 병원균의 분석
Analysis of Waterborne Pathogenic Bacteria among Total Coliform Positive Samples in the Groundwater of Chungcheongnam-do Province, Korea
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초록
Objectives: To ensure the microbiological safety of groundwater, it was confirmed whether waterbornepathogenic bacteria in groundwater samples tested positive for total coliforms in the Chungcheongnam-doProvince region. Methods: Total colony counts, total coliforms and fecal coliforms were tested according to the process mandatedby the drinking water quality testing standards of Korea. DNA was extracted from the samples, tested positivefor total coliforms, and then subjected to real-time PCR to detect waterborne pathogenic bacteria. Results: A total of 115 samples were inadequate for drinking water. Thirty-one cases (27%) showed positive forfecal coliforms and nine cases (7.8%) showed total colony counts exceeding drinking water standards. Twentysevencases (23.5%) showed three items (total colony counts, total coliforms and fecal coliforms). Using the realtimePCR method, waterborne pathogens were detected in 57 cases (49.6%) in 115 samples. Seventy-eight casesof waterborne pathogenic bacteria were detected (including duplications): 27 cases of pathogenic E. coli (EPEC(19), ETEC (5), EHEC (1), EAEC (1) and EIEC (1)); 45 of Bacillus cereus; two of Yersinia spp.; two ofSalmonella spp.; one of Staphylococcus aureus; one of Clostridium perfringens. Conclusion: The real-time PCR method can offer rapid and accurate detection of waterborne pathogenicbacteria. Therefore, this assay could be an alternative to conventional culture methods and can further ensure themicrobiological safety of groundwater.
Abstract
Objectives: To ensure the microbiological safety of groundwater, it was confirmed whether waterbornepathogenic bacteria in groundwater samples tested positive for total coliforms in the Chungcheongnam-doProvince region. Methods: Total colony counts, total coliforms and fecal coliforms were tested according to the process mandatedby the drinking water quality testing standards of Korea. DNA was extracted from the samples, tested positivefor total coliforms, and then subjected to real-time PCR to detect waterborne pathogenic bacteria. Results: A total of 115 samples were inadequate for drinking water. Thirty-one cases (27%) showed positive forfecal coliforms and nine cases (7.8%) showed total colony counts exceeding drinking water standards. Twentysevencases (23.5%) showed three items (total colony counts, total coliforms and fecal coliforms). Using the realtimePCR method, waterborne pathogens were detected in 57 cases (49.6%) in 115 samples. Seventy-eight casesof waterborne pathogenic bacteria were detected (including duplications): 27 cases of pathogenic E. coli (EPEC(19), ETEC (5), EHEC (1), EAEC (1) and EIEC (1)); 45 of Bacillus cereus; two of Yersinia spp.; two ofSalmonella spp.; one of Staphylococcus aureus; one of Clostridium perfringens. Conclusion: The real-time PCR method can offer rapid and accurate detection of waterborne pathogenicbacteria. Therefore, this assay could be an alternative to conventional culture methods and can further ensure themicrobiological safety of groundwater.
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