Proteome Analysis of Escherichia coli after High-dose Radiation
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초록
Since proteomics can be employed to compare changes in the expression levels of manyproteins under particular genetic and environmental conditions, using mass spectrometry to establishradiation stimulon, we performed two-dimensional gel electrophoresis and identified E. coliproteins whose expressions are affected by high dose of ionizing radiation. After exposure to 3 kGy,it was found that 6 proteins involved in carbon and energy metabolism were reduced. Although 4of 7 protein spots showing a significant increase in expression level were neither identified norclassified, uridine phosphorylase (Udp), superoxide dismutase (SodB), and thioredoxin-dependentthiol peroxidase (Bcp) were proven to be up-regulated after irradiation. This suggests that E. colisubjected to high doses of radiation (3 kGy) may operate a defense system that is able to detoxifyreactive oxygen species and stimulate the salvage pathway of nucleotide synthesis to replenish damagedDNA.
Abstract
Since proteomics can be employed to compare changes in the expression levels of manyproteins under particular genetic and environmental conditions, using mass spectrometry to establishradiation stimulon, we performed two-dimensional gel electrophoresis and identified E. coliproteins whose expressions are affected by high dose of ionizing radiation. After exposure to 3 kGy,it was found that 6 proteins involved in carbon and energy metabolism were reduced. Although 4of 7 protein spots showing a significant increase in expression level were neither identified norclassified, uridine phosphorylase (Udp), superoxide dismutase (SodB), and thioredoxin-dependentthiol peroxidase (Bcp) were proven to be up-regulated after irradiation. This suggests that E. colisubjected to high doses of radiation (3 kGy) may operate a defense system that is able to detoxifyreactive oxygen species and stimulate the salvage pathway of nucleotide synthesis to replenish damagedDNA.
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