감마선 조사된 차가버섯 추출물의 암세포 활성 억제
Tumor Cell Cytotoxicity of Gamma Irradiated Chaga Mushroom Extract
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초록
This study was investigated the effect of tumor cell cytotoxicity of gamma irradiatedChaga mushroom extract (CME). CME was prepared by hot water extraction at 70C for 4 hoursand lyophilized. Ten mg ml-1 of lyophilized CME powder was dissolved with deionized water andthen irradiated at the doses of 10, 50, 100, and 150 kGy by cobalt 60 gamma irradiator. The gammairradiatedand non-irradiated CME were treated into the cancer cell, including human stomachcancer and human colon cancer. Cytotoxicity against the cancer cell was increased in gammairradiatedCME and antioxidant activity was also increased in gamma-irradiated CME, as irradiationdose increased. Therefore, it was considered that gamma irradiation was effective method forimprovement of the cancer cell cytotoxicity and antioxidant activity of Chaga mushroom extract.
Abstract
This study was investigated the effect of tumor cell cytotoxicity of gamma irradiatedChaga mushroom extract (CME). CME was prepared by hot water extraction at 70C for 4 hoursand lyophilized. Ten mg ml-1 of lyophilized CME powder was dissolved with deionized water andthen irradiated at the doses of 10, 50, 100, and 150 kGy by cobalt 60 gamma irradiator. The gammairradiatedand non-irradiated CME were treated into the cancer cell, including human stomachcancer and human colon cancer. Cytotoxicity against the cancer cell was increased in gammairradiatedCME and antioxidant activity was also increased in gamma-irradiated CME, as irradiationdose increased. Therefore, it was considered that gamma irradiation was effective method forimprovement of the cancer cell cytotoxicity and antioxidant activity of Chaga mushroom extract.
참고문헌 (21)
- Adamo M, "Truffles decontamination treatment by ionizing radiation", Radiat. Phys. Chem, 71, pp.165~168 (2004)
- Ames BN, "Oxidative DNA damage, cancer and aging. Oxygen and human disease", Ann. Intern. Med, 107, pp.536~539 (1987)
- 이승철, "비단풀 물 추출물의 항산화력 및 항암활성", 한국식품영양과학회지, 35(10), pp.1304~1308 (2006), 한국식품영양과학회
- 안치선, "사염화탄소로 유도된 간 손상 동물모델에서 차가버섯 열수 추출물이 면역 조절 기능에 미치는 영향", 한국약용작물학회지, 18(1), pp.1~8 (2010), 한국약용작물학회
- Blois MS, "Antioxidant activity determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Byrd JC, "Dimerization of the insulin-like growth factor II", mannose 6-phosphate receptor, 2000, pp.25(6), 18647~18656
- Chung KS, "Studies on the antitumor constituents of Pliteus cervinus", Seoul Univ. J. Pharm. Sci, 10, pp.1~18 (1985)
- Cross CE, "Oxygen radicals and human disease", Ann. Intern. Med, 107, pp.526~545 (1987)
- Fitzgerald P, "Retinoic acid receptor α expression correlates with retinoid induced growth inhibition of human breast cancer cells. regardless of estrogen receptor status", Cancer Res, 57, pp.2642~2650 (1997)
- 정명훈, "초고압 추출 공정을 통한 고로쇠 목부 추출물의 항암활성 증진", 한국약용작물학회지, 18(3), pp.157~167 (2010), 한국약용작물학회
- Jo C, "Irradiation application of color removal and purification of green tea leave extract", Radiat. Phys. Chem, 66, pp.179~184 (2003)
- Jo C, "Irradiation effect on color and functional properties of persimmon (Diospyros kaki L. folium) leaf extract and licorice (Glycyrrhiza uralensis Fischer) root extract during storage", Radiat. Phys. Chem, 67, pp.143~148 (2003)
- Kahlos K, "Identification of some lanostane type triterpenes from Inonotus obliquus", Acta. Pham. Fenn, 92, pp.220~224 (1983)
- Kahlos K, "Antitumor activity of some compounds and fractions from an n-hexane extract of Inonotus obliquus", Acta. Pharm. Fenn, 96, pp.33~40 (1987)
- Kahlos K, "Ergosterol peroxide, an active compound from Inonotus radiatus", Planta. Medica, 55, pp.389~390 (1989)
- Kim JH, "γ-Irradiation improves the color and antioxidant properties of Chaga mushroom (Inonotus obliquus) extract", J. Med. Food, 12(6), pp.1343~1347 (2009)
- Kim JK, "Color improvement by irradiation of Curcuma aromatica extract for industrial application", Radiat. Phys. Chem, 75, pp.449~452 (2006)
- Kuo ML, "Curcumin, an antioxidant and antitumor promoter, induces apoptosis in human leukemia cells", Biochim. Biophys. Acta, 1317(10), pp.95~100 (1996)
- Lee IK, "New antioxidant polyphenols from the medicinal mushroom Inonotus obliquus", Bioorg. Med. Chem. Lett, 17, pp.6678~6681 (2007)
- Lee JY, "Effect of Platycodon grandiflorum DC extract on the growth of cancer cell lines", Korean J. Food Sci. Technol, 30(1), pp.13~21 (1998)
- 이승욱, "울릉도산 산채류 추출물의 총 폴리페놀 함량 및 항산화 활성", 한국식품과학회지, 37(2), pp.233~240 (2005), 한국식품과학회