왜성 변이 품종 ‘꼬마’ 무궁화의 캘러스 유도 및 지상부 형성에식물생장조절물질이 미치는 영향
The Effect of Plant Growth Regulators on Callus Induction and Shoot Regeneration from the Dwarf Type Variety, Hibiscus syriacus L.var. Ggoma
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
‘Ggoma’ is a new Hibiscus dwarf type variety developed by gamma irradiation at theKorea Atomic Energy Research Institute (KAERI). This study was conducted to determine thebest optimum cultural callus formation and shoot regeneration condition. Sterilized leaf tissueswere cultured on MS (Marashige and skoog’s) medium containing 3% sucrose, 0.8% agar withdifferent concentration and combination of TDZ, 2, 4-D, KT, BA, and 2iP for 4 weeks in vitro culture. MS medium containing 2,4-D 0.1 mg l-1 and BA 0.5 mg l-1 were most effective on callusformation and growth. After 4 weeks, callus was transferred on BA (0.5, 1, 1.5 mg l-1) and TDZ(0.1, 0.2, 0.3 mg l-1) for shoot formation. The best condition for inducing the shoot from callus wasBA 1.5 mg l-1 and TDZ 0.3 mg l-1. This result will be useful for the rapid multiplication of Hibiscussyriacus L.var. Ggoma.
Abstract
‘Ggoma’ is a new Hibiscus dwarf type variety developed by gamma irradiation at theKorea Atomic Energy Research Institute (KAERI). This study was conducted to determine thebest optimum cultural callus formation and shoot regeneration condition. Sterilized leaf tissueswere cultured on MS (Marashige and skoog’s) medium containing 3% sucrose, 0.8% agar withdifferent concentration and combination of TDZ, 2, 4-D, KT, BA, and 2iP for 4 weeks in vitro culture. MS medium containing 2,4-D 0.1 mg l-1 and BA 0.5 mg l-1 were most effective on callusformation and growth. After 4 weeks, callus was transferred on BA (0.5, 1, 1.5 mg l-1) and TDZ(0.1, 0.2, 0.3 mg l-1) for shoot formation. The best condition for inducing the shoot from callus wasBA 1.5 mg l-1 and TDZ 0.3 mg l-1. This result will be useful for the rapid multiplication of Hibiscussyriacus L.var. Ggoma.
참고문헌 (19)
- "Plant Cell Tissue. Org. Cult.", 1992, 1~5
- An YK, "Studies on vitro shoot regeneration from hypocotyl and cotyledon in Hibiscus syriacus L", Kor. Soc. Hort. Sci, 33, pp.458~465 (1992)
- Brian C, "In vitro culture of Hibiscus rosa-sinensis L.: Influence of iron, calcium and BAP on establishment and multiplication", Plant Cell Tissue. Org. Cult, 93, pp.151~161 (2008)
- Broertjes C, "Mutation breeding of Chrysanthemum", Euphytica, 15, pp.156~162 (1996)
- Chung JD, "Isolation and culture of protoplast of Hibiscus syriacus and Hibiscus rosa sinensis", Kor. J Plant Tissue Culture, 13, pp.71~83 (1986)
- Kim CS, "Some characterstics of tetraploid Hibiscus syriacus for. purpureus induced by colchicine treatment", Ann. Rep. of Inst. of Forest Genetics, 2, pp.57~68 (1962)
- Kim CS, "Review of genetics and breeding in Hibiscus genus", Kor. J. Forestry Sci, 43, pp.64~73 (1979)
- Kim EK, "Thidiazuron-induced shoot formation of Hibiscus syriacus L. ‘Honghwarang’by suspension culture", Kor. J. Hort. Sci. Technol., 16(4), pp.525~527 (1998)
- Kim EK, "Effect of thidiazuron on callus and multiple shoot formation in shoot-tip culture of Hibiscus syriacus L. ‘Honghwarang’", Kor. J. Hort. Sci. Technol, 16(4), pp.520~524 (1998)
- Kim JH, "In vitro regeneration system nucellus of fertilized ovules in Hibiscus syriacus L", J. Agr. Sci, 11, pp.28~38 (2000)
- Preece JE, "Micro and cutting propagation of Silver Maple. I. Results with adult and juvenile propagules", J. Amer. Soc. Hort. Sci, 116, pp.142~148 (1991)
- Shin YU, "Induction of compact mutants in pear(Pyrus pyrifolia Nakai)by gamma irradiation", Res. Report RDA(H), 30, pp.73~78 (1998)
- 강시용, "A Dwarf Type New Rose of Sharon Variety, “Ggoma” Developed by a Mutation Breeding", 한국육종학회지, 38(5), pp.293~294 (2006), 한국육종학회
- Staller LJ, "Mutation in barley induced by X-rays and radium", Science, 69, pp.186~187 (1928)
- Sunil B, "Shoots induction from Hibiscus Rosa-sinensis nodal explant using N6-benzylaminopurine(BAP)", Res. J. Agric &Biol. Sci, 5(4), pp.403~410 (2009)
- Van Harten AM, "In vitro acventitious bud techniques for vegetative propagation and mutation breeding of potato (Solanum tuberosum L.). II", Significance for mutation breeding. Euphytica, 30, pp.1~8 (1981)
- Yang LJ, "In vitro plant regeneration from leaf and petiole explants of Hibiscus syriacus L", Plant Tissue Culture Letters, 12(2), pp.173~177 (1995)
- Yoo EH, "Induction of shoot primordia of Hibiscus syriacus L. ‘Honghwarang’ by suspension culture", J. Kor. Soc. Hort. Sci, 37(4), pp.607~610 (1996)
- Yu TY, "A report of scientific researches in new cultivated varieties of Hibiscus syriacus L", Kor. J. Breed, 11, pp.222~231 (1979)