감마선 및 화학적 돌연변이원 처리가 스테비아(Stevia rebaudiana Bert.)의 종자 발아 및 초기 생장에 미치는 영향
Effects of Gamma-ray and Chemical Mutagens on the Germination and Seedling Growth in Stevia rebaudiana Bert.
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study was carried out to develop the improved useful mutants for yield orcomposition of stevia plants using the gamma ray or chemical mutagens treatments. The seeds ofstevia ‘Suwon No. 11’ were irradiated up to 400 Gy of gamma ray. Chemical mutagens weretreated on the seeds of the ‘Suwon No. 11’ using 0.07% colchicine, 10 mM sodium azide, or 10mM NMU for various durations. The germination rate, and shoot and root growth of seedlingwere estimated at 30 days after gamma ray irradiation or chemical mutagen treatment, and theplant height, the number of branches, and leaf length and width were examined at 3 months aftermutagenesis treatments. In the case of gamma ray treatments, the germination rate and earlystagegrowth were decreased as the increase of radiation dose, and the 50% lethal dose was foundto be 200 Gy. the plant height was decreased as the increase of radiation dose, while the numberof branches per plant and leaf length were increased. Leaf shape was modified to the relativelylonger one compared to the control, which was identified more apparently at the treatments ofhigher than 150 Gy. In the treatment of chemical mutagens, the rate of germination and survivalwere decreased as the increase of incubation time. The 50% lethal dose for germination rate wereidentified as the conditions of the 15 hours incubation in 0.07% colchicine, the 4 hrs in 10 mMsodium azide, and the 2 hrs in 10 mM NMU, in the three chemical mutagens treatments. Chemicalmutagens had no influence on shoot growth, while root growth was increased, especially as theincubation time was extended. The highest root growth occurred in the NMU treatment at 6 hrsincubation time. The plant height was decreased as the increase of incubation time in the chemicalmutagens treatments. Among the chemical mutagens, NMU was the most effective to induce themutants with long-shaped or the least lobed leaves.
Abstract
This study was carried out to develop the improved useful mutants for yield orcomposition of stevia plants using the gamma ray or chemical mutagens treatments. The seeds ofstevia ‘Suwon No. 11’ were irradiated up to 400 Gy of gamma ray. Chemical mutagens weretreated on the seeds of the ‘Suwon No. 11’ using 0.07% colchicine, 10 mM sodium azide, or 10mM NMU for various durations. The germination rate, and shoot and root growth of seedlingwere estimated at 30 days after gamma ray irradiation or chemical mutagen treatment, and theplant height, the number of branches, and leaf length and width were examined at 3 months aftermutagenesis treatments. In the case of gamma ray treatments, the germination rate and earlystagegrowth were decreased as the increase of radiation dose, and the 50% lethal dose was foundto be 200 Gy. the plant height was decreased as the increase of radiation dose, while the numberof branches per plant and leaf length were increased. Leaf shape was modified to the relativelylonger one compared to the control, which was identified more apparently at the treatments ofhigher than 150 Gy. In the treatment of chemical mutagens, the rate of germination and survivalwere decreased as the increase of incubation time. The 50% lethal dose for germination rate wereidentified as the conditions of the 15 hours incubation in 0.07% colchicine, the 4 hrs in 10 mMsodium azide, and the 2 hrs in 10 mM NMU, in the three chemical mutagens treatments. Chemicalmutagens had no influence on shoot growth, while root growth was increased, especially as theincubation time was extended. The highest root growth occurred in the NMU treatment at 6 hrsincubation time. The plant height was decreased as the increase of incubation time in the chemicalmutagens treatments. Among the chemical mutagens, NMU was the most effective to induce themutants with long-shaped or the least lobed leaves.
참고문헌 (30)
- Arenaz P, "Effect of sodium azide on sisterchromatid exchanges in human lymphocytes and Chinese hamster cell", Mutation Research, 88, pp.217~221 (1981)
- Castillo AM, "Efficient production of androgenic doubled-haploid mutants in barley by the application of sodium azide to anther and microspore culture", Plant Cell Rep, 20, pp.105~111 (2001)
- Chalapathi MV, "Natural non-caloric sweetener stevia(Stevia rebaudiana Bertoni) : A future crop of India", Crop Res. Hisar, 14(2), pp.347~350 (1997)
- Choi NH, "Morphological characteristics of Campanula punctata Lam. var. rubriflora Mak. as affected by doses of γ-ray radiation", Flower Res. J., 14(2), pp.116~120 (2006)
- Doo HS, "Induced mutation by gamma-ray irradiation on crown bud of yacon(Polymnia sonchifolia Poeppig & Endlicher)", Korean J. Breed, 33(1), pp.1~6 (2001)
- 구대희, "감마선 처리에 의한 국화의 화색 변화", Horticulture, Environment, and Biotechnology, 44(6), pp.1006~1009 (2003), 한국원예학회
- Hanson JR, "Stevioside and related sweet diterpenoid glycosides", Nat. Prod. Rep, 10, pp.301~309 (1993)
- 정진아, "γ-선 및 화학돌연변이원 처리가 양치식물 전엽체의 기내배양에 미치는 영향", 한국자원식물학회지, 19(2), pp.348~25 (2006), 한국자원식물학회
- 정진아, "기내에서 화학돌연변이원 처리에 의한 참쇠고비의 변이주 유기 및 RAPD 분석", 한국자원식물학회지, 19(2), pp.374~29 (2006), 한국자원식물학회
- Jin YH, "Investigation of optimal concentration and treatment period of colchicine for mutation breeding of Lilium spp", Kor. J. Hort. Sci. Technol, 21(Suppl. II), pp.112~ (2003)
- Katayama O, "The practical application of stevia and research and development data", pp.747~ (1976), ISU Company
- Kim JS, "Ionizing radiation hormesis in crops", Kor. J. Environ. Agri, 17(1), pp.76~85 (1998)
- Kinghorn AD, "Discovery of terpenoid and phenolic sweeteners from plants", Pure. Appl. Chem, 74(7), pp.1169~1179 (2002)
- Koh JC, "Effect of colchicine treatment for polyploidy induction of Iris ensata", Kor. J. Hort. Sci. Technol, 19(Suppl. II), pp.91~ (2001)
- Kwon SH, "Recommendable dose of X-and gamma-ray for mutation breeding", Korean J. Breed, 10(2), pp.127~132 (1978)
- 이혜정, "잔디류 돌연변이 유기를 위한 적정 방사선 선량범위의 결정", Weed & Turfgrass Science, 22(1), pp.25~34 (2008), 한국잔디학회
- Lee JI, "High-yielding stevia variety “Suweon 2”", Res. Rept. ORD, 21, pp.167~169 (1979)
- Lee JI, "New high rebaudioside-A stevia variety “Suweon 11”", Res. Rept. ORD, 24, pp.186~188 (1982)
- Lee KH, "Variations in mutant plants by chemical mutagen treatments of Dianthus superbus L", Korean J. Medicinal Crop Sci, 8(4), pp.334~341 (2000)
- Lim JD, "Induction and RAPD analysis of mutant plants by chemical mutagens in Gentiana axillariflora Leveille", Kor. J. Plant Tiss. Cult, 27, pp.89~94 (2000)
- Nishiyama P, "Quantitative analysis of stevioside in the leaves of Stevia rebaudiana by near infrared reflectance spectroscopy", J. Sci. Food Agri, 59(3), pp.277~281 (1992)
- Oh BK, "Gamma radiation sensitivity and quantitative characters in M1 generation of Heteropappus hispidus (Thunb.)", Korean J. Environ. Agri, 22(4), pp.294~299 (2003)
- 허북구, "감마선조사가옥잠화와털머위종자의발아, 생육및변이유발에미치는영향", 화훼연구, 16(2), pp.128~133 (2008), 한국화훼학회
- 박재옥, "술패랭이꽃과 층꽃나무 종자에 대한 감마선 조사가 발아, 생육 및 변이유발에 미치는 영향", 한국자원식물학회지, 23(1), pp.19~24 (2010), 한국자원식물학회
- Sachs RM, "Chemical inhibition of plant height", Hort. Sci, 2, pp.440~447 (1972)
- Schum A, "Somaclonal variation and induced mutation in crop improvement", pp.332~366 (1998), Kluwer Academic Publ
- Seong NS, "In vitro variant induction and its content of gentiopicroside of Gentiana scabra Bunge", Kor. J. Med. Crop, 3, pp.40~44 (1995)
- Shin JY, "Effects of gamma ray on the radiosensitivity of safflower (Carthamus tinctorius L.)", J. Honam Soc. Hort. Sci, 3, pp.93~99 (2005)
- Soejarto DD, "Potential sweetening agents of plant origin II. Field search for sweet-tasting Stevia species", Econ. Bot, 37, pp.71~79 (1983)
- 서종택, "자생 중나리 종자의 콜히친 처리에 따른 변이체 특성", 원예과학기술지, 23(4), pp.430~432 (2005), 한국원예학회