감마선 조사가 감초(Glycyrrhiza uralensis)의 초기 생육 및DNA 손상에 미치는 영향
Effects of Gamma-ray Irradiation on Growth Characteristics and DNA Damage in Licorice (Glycyrrhiza uralensis)
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This study was conducted to determine the optimal dose of gamma-ray on the growthand nucleus DNA damage for mutation breeding in licorice. Gamma-rays irradiated to dry seedswith various doses (0 to 1000 Gy). Significant decreases in germination rate (%), survival rate (%)and growth characteristics (plant height, number of leaves, root length and fresh weight) wereobserved by dose of increased. LD50 (lethal dose) was approximately 400 Gy to 500 Gy. Also, reductiondoses (RD50) of plant height, number of leaves, root length and flash weight were 428 Gy, 760Gy, 363 Gy and 334 Gy, respectively. It is supplest that the optimal dose of gamma irradiation forlicorice mutation induction might be about 400 Gy in this study. We also conducted comet assayto observe nucleus DNA damage due to gamma irradiation. In comet assay, a clear difference wasidentified over 300 Gy treatments. With increasing doses of gamma-ray in the range of 100 to 1000Gy, the rate of head DNA was decreased significantly from 92.88% to 73.09%. Tail length (μm) wasincreased as the dose of increased over 300 Gy. Growth characteristics (Germination rate, Survivalrate, plant height, number of leaves, root length and fresh weight) were highly negatively (P‹0.01)correlated with dose. While the tail length was highly positively (P‹0.01) correlated with dose.
Abstract
This study was conducted to determine the optimal dose of gamma-ray on the growthand nucleus DNA damage for mutation breeding in licorice. Gamma-rays irradiated to dry seedswith various doses (0 to 1000 Gy). Significant decreases in germination rate (%), survival rate (%)and growth characteristics (plant height, number of leaves, root length and fresh weight) wereobserved by dose of increased. LD50 (lethal dose) was approximately 400 Gy to 500 Gy. Also, reductiondoses (RD50) of plant height, number of leaves, root length and flash weight were 428 Gy, 760Gy, 363 Gy and 334 Gy, respectively. It is supplest that the optimal dose of gamma irradiation forlicorice mutation induction might be about 400 Gy in this study. We also conducted comet assayto observe nucleus DNA damage due to gamma irradiation. In comet assay, a clear difference wasidentified over 300 Gy treatments. With increasing doses of gamma-ray in the range of 100 to 1000Gy, the rate of head DNA was decreased significantly from 92.88% to 73.09%. Tail length (μm) wasincreased as the dose of increased over 300 Gy. Growth characteristics (Germination rate, Survivalrate, plant height, number of leaves, root length and fresh weight) were highly negatively (P‹0.01)correlated with dose. While the tail length was highly positively (P‹0.01) correlated with dose.
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