감마선에 의한 산화적 스트레스에 돌연변이 약용들깨 열수추출물의방호 효능
Protective Effect of Perilla frutescens cv. Chookyoupjaso Mutant Water Extract on Gamma Ray-induced Oxidative Stress in Mice
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The purpose of this study was to evaluate the protective effect of the Perilla frutescenscv. Chookyoupjaso mutant water extract (PFWE) on gamma ray-induced oxidative stress in mice. Gamma-ray is one of the sources for inducing oxidative stress. The study was divided into 6 groupswith 6 mice for each treatment. Groups I and II were treated with saline (vehicle) only, groups III,IV, V, and VI were pretreated with PFWE 10, 20, 50, 50 mg kg-1 respectively for 2 weeks beforegamma radiation. And then groups II, III, IV, V were irradiated. We found that the activities ofaspartate transaminase (AST) and alanine transaminase (ALT) were increased and the activities ofantioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase(GPx), and glutathione reductase (GR) were decreased by irradiation in mice. However, treatmentof PFWE attenuated the activities of AST and ALT in a dose-dependent manner in irradiated mice. Furthermore, treatment of PFWE significantly increased the activities of SOD, GPx, and GR in adose-dependent manner in irradiated mice, except for the CAT. Interestingly, the activities of GPxand GR were significantly increased by PFWE treatment. Taken together, PFWE could be effectivein protecting on gamma ray-induced oxidative stress in mice.
Abstract
The purpose of this study was to evaluate the protective effect of the Perilla frutescenscv. Chookyoupjaso mutant water extract (PFWE) on gamma ray-induced oxidative stress in mice. Gamma-ray is one of the sources for inducing oxidative stress. The study was divided into 6 groupswith 6 mice for each treatment. Groups I and II were treated with saline (vehicle) only, groups III,IV, V, and VI were pretreated with PFWE 10, 20, 50, 50 mg kg-1 respectively for 2 weeks beforegamma radiation. And then groups II, III, IV, V were irradiated. We found that the activities ofaspartate transaminase (AST) and alanine transaminase (ALT) were increased and the activities ofantioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase(GPx), and glutathione reductase (GR) were decreased by irradiation in mice. However, treatmentof PFWE attenuated the activities of AST and ALT in a dose-dependent manner in irradiated mice. Furthermore, treatment of PFWE significantly increased the activities of SOD, GPx, and GR in adose-dependent manner in irradiated mice, except for the CAT. Interestingly, the activities of GPxand GR were significantly increased by PFWE treatment. Taken together, PFWE could be effectivein protecting on gamma ray-induced oxidative stress in mice.
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