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방사선 기반에 의한 기계적으로 공극을 증가시킨 젤라틴이 도입된혈관조직공학용 PLLA/PLCL 나노섬유 지지체의 개발

Development of Mechanically Expanded Gelatin-AAc- PLLA/PLCL Nanofibers for Vascular Tissue Engineering by Radiation-based Techniques

박종석|안성준|신영민|권희정|임윤묵|정성인|정진오|서다은
(사)한국방사선산업학회 (Korean Society of Radiation Industry)|2015.12|방사선산업학회지 (Journal of Radiation Industry)|제9권 제4호|pp. 171-180|DOI 10.23042/radin.2015.9.4.171

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키워드

GelatinGamma-irradiationPoly(L-lactidePLLA)Poly(L-lactide-co-ε-caprolactonePLCL)Vascular tissue engineering

초록

Vascular tissue engineering has been accessed to mimic the natural composition ofthe blood vessel containing intima, media, and adventitia layers. We fabricated mechanicallyexpanded PLLA/PLCL nanofibers using electrospinning and UTM. The pore size of the mesheswas increased the gelatin immobilized AAc-PLLA/PLCL nanofibers (203.30±49.62 microns)than PLLA/PLCL nanofibers (59.99±8.66 microns) after mechanical expansion. To increasethe cell adhesion and proliferation, we introduced carboxyl group, and gelatin was conjugatedon them. The properties of the PLLA/PLCL nanofibers were analyzed with SEM, ATR-FTIR,TBO staining, and water contact angle measurement, general cell responses on the PLLA/PLCLnanofibers such as adhesion, proliferation, and infiltration were also investigated using smoothmuscle cell (SMC). During the SMC culture, the initial viability of the cells was significantlyincreased on the gelatin immobilized AAc-PLLA/PLCL nanofibers, and infiltration of the cellswas also enhanced on them. Therefore, gelatin immobilized AAc-PLLA/PLCL nanofibers andmechanically expanded meshes may be a good tool for vascular tissue engineering application.

Abstract

Vascular tissue engineering has been accessed to mimic the natural composition ofthe blood vessel containing intima, media, and adventitia layers. We fabricated mechanicallyexpanded PLLA/PLCL nanofibers using electrospinning and UTM. The pore size of the mesheswas increased the gelatin immobilized AAc-PLLA/PLCL nanofibers (203.30±49.62 microns)than PLLA/PLCL nanofibers (59.99±8.66 microns) after mechanical expansion. To increasethe cell adhesion and proliferation, we introduced carboxyl group, and gelatin was conjugatedon them. The properties of the PLLA/PLCL nanofibers were analyzed with SEM, ATR-FTIR,TBO staining, and water contact angle measurement, general cell responses on the PLLA/PLCLnanofibers such as adhesion, proliferation, and infiltration were also investigated using smoothmuscle cell (SMC). During the SMC culture, the initial viability of the cells was significantlyincreased on the gelatin immobilized AAc-PLLA/PLCL nanofibers, and infiltration of the cellswas also enhanced on them. Therefore, gelatin immobilized AAc-PLLA/PLCL nanofibers andmechanically expanded meshes may be a good tool for vascular tissue engineering application.

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