Improvement of Cellulose Degradation via Highly Expressed LebglA in Radiation Induced Mutant of Lentinula edodes
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Cellulose degradation improved mutants, LER120, LER121, LER277R80, LER277R88, and LER277R140 were induced from Lentinula edodes KACC42378 using gamma ray radiation (60Co) at the dose of LD99 (0.94kGy). To understand the cellulase activity enhanced mechanism in these mutants, we examined the expression of cellulase genes, cellobiohydrolase (cel7A and cel6B), endoglucanase (Lecel5A and Lecel12A) and β-glucosidase (Lxyl-p1 and LebglA) using the qRT-PCR. The expression of LebglA was significantly up-regulated in all mutants (LER120; 6.5 fold, LER121; 10.2 fold, LER277R80; 2.0 fold, LER277R88; 17.6 fold, LER277R140; 18.5 fold) compared with that of the wild type (WT) in the 1% carboxymethylcellulose (CMC) media, while the expression of cellobiohydrolases and endoglucanases did not changed. To clarify the mutation on the sequences of the LebglA by radiation, we cloned LebglA coding DNA sequence (CDS). There was no sequence variations between LebglA CDSs of each mutant and WT. Based on commonly up-regulation of LebglA expression in all cellulose degradation improved mutants, LebglA could play a principal role in cellulose degradation of L. edodes and β-glucosidase specific regulator gene(s) might be turned on in mutants in this study.
Abstract
Cellulose degradation improved mutants, LER120, LER121, LER277R80, LER277R88, and LER277R140 were induced from Lentinula edodes KACC42378 using gamma ray radiation (60Co) at the dose of LD99 (0.94kGy). To understand the cellulase activity enhanced mechanism in these mutants, we examined the expression of cellulase genes, cellobiohydrolase (cel7A and cel6B), endoglucanase (Lecel5A and Lecel12A) and β-glucosidase (Lxyl-p1 and LebglA) using the qRT-PCR. The expression of LebglA was significantly up-regulated in all mutants (LER120; 6.5 fold, LER121; 10.2 fold, LER277R80; 2.0 fold, LER277R88; 17.6 fold, LER277R140; 18.5 fold) compared with that of the wild type (WT) in the 1% carboxymethylcellulose (CMC) media, while the expression of cellobiohydrolases and endoglucanases did not changed. To clarify the mutation on the sequences of the LebglA by radiation, we cloned LebglA coding DNA sequence (CDS). There was no sequence variations between LebglA CDSs of each mutant and WT. Based on commonly up-regulation of LebglA expression in all cellulose degradation improved mutants, LebglA could play a principal role in cellulose degradation of L. edodes and β-glucosidase specific regulator gene(s) might be turned on in mutants in this study.
참고문헌 (18)
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