Enhanced β-Glucosidase Production by Penicillium simplicissimum Mutant Strains under Solid State Fermentation
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Cellulase enzymes play a key role in degrading cellulose to produce sugars, with agricultural materials serving as primary sources after lignin removal. Fungi are efficient producers of cellulolytic enzymes, including cellulase. Penicillium JLP3201 was a potential strain that produced endocellulase, exocellulase and β-glucosidase at 3.58, 1.52 and 13.20 U/g, respectively. It produced the highest endocellulase and βglucosidase in solid state fermentation of 1% NaOH-pretreated rain tree leaves, which outperformed other substrates. All enzymes indicated the optimum condition at pH 4.0 and 50℃; β-glucosidase was less stable above 60℃ after one hour. The fungal strain was mutated by UVC radiation, called Penicillium MU8-0, that demonstrated higher endocellulase and β-glucosidase at 4.91 and 66.00 U/g, respectively, achieving a 5-fold increase in β-glucosidase. The enzyme properties of the mutant strain changed, showing stability at pH 7.0 and higher temperature tolerance up to 70℃.
Abstract
Cellulase enzymes play a key role in degrading cellulose to produce sugars, with agricultural materials serving as primary sources after lignin removal. Fungi are efficient producers of cellulolytic enzymes, including cellulase. Penicillium JLP3201 was a potential strain that produced endocellulase, exocellulase and β-glucosidase at 3.58, 1.52 and 13.20 U/g, respectively. It produced the highest endocellulase and βglucosidase in solid state fermentation of 1% NaOH-pretreated rain tree leaves, which outperformed other substrates. All enzymes indicated the optimum condition at pH 4.0 and 50℃; β-glucosidase was less stable above 60℃ after one hour. The fungal strain was mutated by UVC radiation, called Penicillium MU8-0, that demonstrated higher endocellulase and β-glucosidase at 4.91 and 66.00 U/g, respectively, achieving a 5-fold increase in β-glucosidase. The enzyme properties of the mutant strain changed, showing stability at pH 7.0 and higher temperature tolerance up to 70℃.