Cloning of Cellobiohydrolase Gene (cbhI) in Radiation Induced Mutant of Pleurotus florida
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The cellobiohydrolase gene (cbhI), a key component of a cellulolytic system, of a mutantPfCM4 (Pleurotus florida), developed through gamma ray radiation mutagenesis, was isolated andcloned. The deduced amino acid sequence was closely related to the glycoside hydrolase family 7(GH7). The molecular weight of the deduced amino acid sequence of cbhI gene was found to be22.4 kDa. Though the percent identity was found to be much less (35.61%) between the wild typeand mutant, the cellulolytic activity of PfCM4 was 17.24% higher than that of the wild type. Thisshows that the catalytic domain of the cbhI gene was conserved in the mutant PfCM4.
Abstract
The cellobiohydrolase gene (cbhI), a key component of a cellulolytic system, of a mutantPfCM4 (Pleurotus florida), developed through gamma ray radiation mutagenesis, was isolated andcloned. The deduced amino acid sequence was closely related to the glycoside hydrolase family 7(GH7). The molecular weight of the deduced amino acid sequence of cbhI gene was found to be22.4 kDa. Though the percent identity was found to be much less (35.61%) between the wild typeand mutant, the cellulolytic activity of PfCM4 was 17.24% higher than that of the wild type. Thisshows that the catalytic domain of the cbhI gene was conserved in the mutant PfCM4.
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