방사선·알칼리 전처리 볏짚 당화액 및 당밀 활용Desmodesmus sp. KAERI-NJ5의 지질 생산성 비교 평가
Evaluation of Lipid Productivity of Desmodesmus sp. KAERI-NJ5 using Radiation and Alkali Pretreated Rice Straw Hydrolysate and Molasses
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Depending on species, microalgae can produce fatty acids more efficiently byusing organic carbon sources than by using the phototrophic cultivation mode. In this study,Desmodesmus sp. KAERI-NJ5 was grown in heterotrophic (HT) and mixotrophic (MT) modesusing enzymatic hydrolysate of rice straw pretreated with radiation and alkali solution to enhancebiodiesel feedstock productivity. In addition, biomass and lipid productivities were comparedwith the results from molasses based medium. Firstly, carbon source preference of the microalgawas determined. Although the presence of light caused a slight difference in the carbon sourcepreference, glucose was the most preferred carbon source in both types of cultivation. The ricestraw hydrolysate (RSH) was used as a carbon source and successfully utilized by the microalga. The biomass and lipid yield was higher in the HT cultivation mode than those from the MTcultivation mode. The final biomass and lipid yield of 6.0 and 3.4 g·l-1 was achieved, respectively,in HT cultivation. In addition, the conversion yield of consumed sugars to lipid and fatty acidmethyl esters (FAMEs) were 34.2 and 31.4%, respectively, which is even higher than that grownwith corresponding pure sugars. Analysis of the FAMEs composition revealed that palmitic, oleic,and stearic acids were the main FAMEs, and the FAMEs content was 52.19% in the HT cultivationmode corresponding to a 1.51-fold higher content than with MT cultivation. In addition, 92.52%of FAMEs consisted of saturated and monounsaturated fatty acids indicating good qualitybiodiesel feedstock. In addition, the productivities of biomass, total lipid and FAMEs were also evaluated when the microalga was grown with molasses, a representative low-cost carbon source,in both cultivation modes. The highest total lipid yield was 3.9 g·l-1 in the HT cultivation modecorresponding to 1.15-fold higher than that of the RSH grown microalga. However, the lipidproductivity was negligible considering the quality as a biodiesel feedstock, regional availabilityand sustainability of carbon sources. Thus, the RSH could be used as a carbon source for biodieselfeedstock production by Desmodesmus sp. KAERI-NJ5.
Abstract
Depending on species, microalgae can produce fatty acids more efficiently byusing organic carbon sources than by using the phototrophic cultivation mode. In this study,Desmodesmus sp. KAERI-NJ5 was grown in heterotrophic (HT) and mixotrophic (MT) modesusing enzymatic hydrolysate of rice straw pretreated with radiation and alkali solution to enhancebiodiesel feedstock productivity. In addition, biomass and lipid productivities were comparedwith the results from molasses based medium. Firstly, carbon source preference of the microalgawas determined. Although the presence of light caused a slight difference in the carbon sourcepreference, glucose was the most preferred carbon source in both types of cultivation. The ricestraw hydrolysate (RSH) was used as a carbon source and successfully utilized by the microalga. The biomass and lipid yield was higher in the HT cultivation mode than those from the MTcultivation mode. The final biomass and lipid yield of 6.0 and 3.4 g·l-1 was achieved, respectively,in HT cultivation. In addition, the conversion yield of consumed sugars to lipid and fatty acidmethyl esters (FAMEs) were 34.2 and 31.4%, respectively, which is even higher than that grownwith corresponding pure sugars. Analysis of the FAMEs composition revealed that palmitic, oleic,and stearic acids were the main FAMEs, and the FAMEs content was 52.19% in the HT cultivationmode corresponding to a 1.51-fold higher content than with MT cultivation. In addition, 92.52%of FAMEs consisted of saturated and monounsaturated fatty acids indicating good qualitybiodiesel feedstock. In addition, the productivities of biomass, total lipid and FAMEs were also evaluated when the microalga was grown with molasses, a representative low-cost carbon source,in both cultivation modes. The highest total lipid yield was 3.9 g·l-1 in the HT cultivation modecorresponding to 1.15-fold higher than that of the RSH grown microalga. However, the lipidproductivity was negligible considering the quality as a biodiesel feedstock, regional availabilityand sustainability of carbon sources. Thus, the RSH could be used as a carbon source for biodieselfeedstock production by Desmodesmus sp. KAERI-NJ5.
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