Enhanced Production of Microalgal Biomass and Fatty Acids via Optimization of Culture Conditions from Indigenous Freshwater Green Microalga, Micratinium sp.
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The aim of this study was to improve microalgal biomass and fatty acid (FA) productivities using the newly isolated freshwater microalga Micratinium sp. KGP-12 via sequential optimization of culture conditions. First, the appropriate temperature and light intensity for Micratinium sp. were determined as 30℃ and 300 μmol/m2 /s, respectively, using a high throughput photobioreactor. The Box-Behnken experimental design technique was adopted to optimize the culture medium by adjusting the N, P, and Mg concentrations in BG-11. The biomass and fatty acid productivities from optimized BG-11 increased by up to 45%, compared to those from standard BG-11 in 0.5 L bubble column photobioreactors. Analysis of the FA composition did not show any differences in either composition or content. When the cells were subjected to Nand/or P-deficient media to increase cellular FA content, N deficiency groups (N-P+ and N-P-) presented a fast increase in FA content from 8.8% to 13.6% on day 2, whereas that of P-deficient medium was only 9.8%. Overall, the results indicated that biomass and FA productivities could be effectively enhanced via the optimization of culture medium and appropriate nutrient supply strategies.
Abstract
The aim of this study was to improve microalgal biomass and fatty acid (FA) productivities using the newly isolated freshwater microalga Micratinium sp. KGP-12 via sequential optimization of culture conditions. First, the appropriate temperature and light intensity for Micratinium sp. were determined as 30℃ and 300 μmol/m2 /s, respectively, using a high throughput photobioreactor. The Box-Behnken experimental design technique was adopted to optimize the culture medium by adjusting the N, P, and Mg concentrations in BG-11. The biomass and fatty acid productivities from optimized BG-11 increased by up to 45%, compared to those from standard BG-11 in 0.5 L bubble column photobioreactors. Analysis of the FA composition did not show any differences in either composition or content. When the cells were subjected to Nand/or P-deficient media to increase cellular FA content, N deficiency groups (N-P+ and N-P-) presented a fast increase in FA content from 8.8% to 13.6% on day 2, whereas that of P-deficient medium was only 9.8%. Overall, the results indicated that biomass and FA productivities could be effectively enhanced via the optimization of culture medium and appropriate nutrient supply strategies.