미세조류 옥외배양 시스템을 이용한 돈분 액체 비료의 영양염류 제거 및 바이오디젤 생산
Biodiesel Production and Nutrients Removal from Piggery Manure Using Microalgal Small Scale Raceway Pond (SSRP)
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Due to the rapid energy consumption and fossil fuel abundance reduction, the world isprogressively in need of alternative and renewable energy sources such as biodiesel. Biodiesel frommicroalgae offers high hopes to the scientific world for its potential as well as its non-competitionwith arable lands. Taking consideration to reduce the cost of production as well as to attain twinenvironmental goals of treatment and use of animal waste material the microalgal cultivation usingpiggery manure has been tested in this study. Unialgal strains such as Chlorella sp. JK2, Scenedesmussp. JK10, and an indigenous mixed microalgal culture CSS were cultured for 20 days in dilutedpiggery manure using Small Scale Raceway Pond (SSRP). Biomass production and lipid productivityof CSS were 1.19±0.09 g L-1, 12.44±0.38 mg L-1 day-1, respectively and almost twice that of unialgalstrains. Also, total nitrogen and total phosphorus removal efficiencies of CSS was 93.6% and98.5% respectively and 30% higher removal efficiency compared to the use of unialgal strains. These results indicate that the piggery manure can provide microalgae necessary nitrogen andphosphorus for growth thereby effectively treating the manure. In addition, overall cost of microalgalcultivation and subsequently biodiesel production would be significantly reduced.
Abstract
Due to the rapid energy consumption and fossil fuel abundance reduction, the world isprogressively in need of alternative and renewable energy sources such as biodiesel. Biodiesel frommicroalgae offers high hopes to the scientific world for its potential as well as its non-competitionwith arable lands. Taking consideration to reduce the cost of production as well as to attain twinenvironmental goals of treatment and use of animal waste material the microalgal cultivation usingpiggery manure has been tested in this study. Unialgal strains such as Chlorella sp. JK2, Scenedesmussp. JK10, and an indigenous mixed microalgal culture CSS were cultured for 20 days in dilutedpiggery manure using Small Scale Raceway Pond (SSRP). Biomass production and lipid productivityof CSS were 1.19±0.09 g L-1, 12.44±0.38 mg L-1 day-1, respectively and almost twice that of unialgalstrains. Also, total nitrogen and total phosphorus removal efficiencies of CSS was 93.6% and98.5% respectively and 30% higher removal efficiency compared to the use of unialgal strains. These results indicate that the piggery manure can provide microalgae necessary nitrogen andphosphorus for growth thereby effectively treating the manure. In addition, overall cost of microalgalcultivation and subsequently biodiesel production would be significantly reduced.
참고문헌 (38)
- Altin R, "The potential of using vegetable oil fuels as fuel for diesel engines", Energy Conv. Manag, 42, pp.529~538 (2001)
- APHA., "Standard methods for the examination of water and wastewater", ~ (2005)
- Barsanti L, "Algae: anatomy, biochemistry, and biotechnology" (2005), CRC press
- Bligh EG, "A rapid method of total lipid extraction and purification", Can. J. Biochem. Phys, 37, pp.911~917 (1959)
- Cai T, "Nutrient recovery from wastewater streams by microalgae: Status and prospects", Renew. Sust. Energ. Rev, 19, pp.360~369 (2013)
- Cain J, "Toxicity and bioaccumulation of cadmium in the colonial green alga Scenedesmus obliquus", Arch. Environ. Contam. Toxicol, 9, pp.9~16 (1980)
- Chisti Y., "Biodiesel from microalgae", Biotechnol. Adv, 25, pp.294~306 (2007)
- Demirbas A., "Comparison of transesterification methods for production of biodiesel from vegetable oils and fats", Energy Conv. Manag, 49, pp.125~130 (2008)
- Felizardo P, "Production of biodiesel from waste frying oils", Waste Manage, 26, pp.487~494 (2006)
- Georgianna DR, "Exploiting diversity and synthetic biology for the production of algal biofuels", Nature, 488, pp.329~335 (2012)
- Gerpen JV., "Biodiesel processing and production", Fuel Process. Technol, 86, pp.1097~1107 (2005)
- González LE, "Efficiency of ammonia and phosphorus removal from a colombian agroindustrial wastewater by the microalgae Chlorella vulgaris and Scenedesmus dimorphus", Bioresour. Technol, 60, pp.259~262 (1997)
- Guerrero MG, "The assimilatory nitrate-reducing system and its regulation", Ann. Rev. Plant Physiol, 32, pp.169~204 (1981)
- Hu Q, "Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances", Plant J, 54, pp.621~639 (2008)
- MijeongLeeJeong, "Carbon Dioxide Mitigation by Microalgal Photosynthesis", Bulletin of the Korean Chemical Society, 24(12), pp.1763~1766 (2003), 대한화학회
- 강시온, "미세조류 옥외 배양시스템을 이용한 바이오디젤 생산 및 도시하수 영양 염류 제거", 한국미생물·생명공학회지, 41(2), pp.207~214 (2013), 한국미생물·생명공학회[DOI]
- 강시온, "미세조류 옥외 배양시스템을 이용한 도시하수 정화 및 미생물 군집다양성 분석", 미생물학회지, 48(3), pp.192~199 (2012), 한국미생물학회[DOI]
- Lee J, "Microalgae-associated bacteria play a key role in the flocculation of Chlorella vulgaris", Bioresour. Technol, 131, pp.195~201 (2013)
- Lee J-Y, "Comparison of several methods for effective lipid extraction from microalgae", Bioresour. Technol, 101, pp.S75~S77 (2010)
- 이상복, "계화간척지에서 돈분뇨 퇴⋅액비 시용이 청보리 (Hordeum vulgare L.) 수량 및 토양화학성에 미치는 영향", 한국토양비료학회지(Korean Journal of Soil Science and Fertilizer), 44(3), pp.353~360 (2011), 한국토양비료학회
- Ma F, "Biodiesel production: a review", Bioresour. Technol, 70, pp.1~15 (1999)
- Mandal S, "Microalga Scenedesmus obliquus as a potential source for biodiesel production", Appl. Microbiol. Biotechnol, 84, pp.281~291 (2009)
- Discharges amounts of livestock manure and resource recycling. Ministry of Food, Agriculture, Forestry and Fisheries. Seoul. Korea, "MIFAFF.", ~ (2010)
- Morris I, "The development of nitrate reductase in Chlorella and its repression by ammonium", Archiv. Mikrobiol, 47, pp.32~41 (1963)
- Omar HH., "Bioremoval of zinc ions by Scenedesmus obliquus and Scenedesmus quadricauda and its effect on growth and metabolism", Int. Biodeterior. Biodegrad, 50, pp.95~100 (2002)
- 박진면, "돈분 액비 관비가 오이의 생육 및 토양화학성에 미치는 영향", 한국토양비료학회지(Korean Journal of Soil Science and Fertilizer), 44(2), pp.194~199 (2011), 한국토양비료학회
- Phan AN, "Biodiesel production from waste cooking oils.", Fuel, 87, pp.3490~3496 (2008)
- Pittman JK, "The potential of sustainable algal biofuel production using wastewater resources", Bioresour. Technol, 102, pp.17~25 (2011)
- Scragg AH, "The use of a fuel containing Chlorella vulgaris in a diesel engine", Enzyme Microb. Technol, 33, pp.884~889 (2003)
- Sharma YC, "Advancements in development and characterization of biodiesel: A review", Fuel, 87, pp.2355~2373 (2008)
- Smith F, "Regulation of nitrate reductase in Chlorella vulgaris", Plant Physiol, 48, pp.224~ (1971)
- Smith VH, "The ecology of algal biodiesel production", Trends Ecol. Evol, 25, pp.301~309 (2010)
- Spolaore P, "Commercial applications of microalgae", J. Biosci. Bioeng, 101, pp.87~96 (2006)
- Stockenreiter M, "The effect of species diversity on lipid production by microalgal communities", J. Appl. Phycol, 24, pp.45~54 (2012)
- Syrett PJ, "The inhibition of nitrate assimilation by ammonium in chlorella", Biochim. Biophys. Acta, 67, pp.566~575 (1963)
- Weis JJ, "Effects of algal diversity on the production of biomass in homogeneous and heterogeneous nutrient environments: a microcosm experiment", PLoS one, 3, pp.e2825~ (2008)
- Yun Y-S, "Enhancement of CO2tolerance of Chlorella vulgaris by gradual increase of CO2concentration", Biotechnol. Tech, 10, pp.713~716 (1996)
- Yun YS, "Carbon dioxide fixation by algal cultivation using wastewater nutrients.", J. Chem. Technol. Biotechnol, 69, pp.451~455 (1997)