Application of Lignocellulosic and Macro-algae Hydrolysates for Culture of Chlorella saccharophila
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In this study, we investigated the possibility of using hydrolysates of lignocellulosics (rapeseed straw, barley straw, rice straw) and marine macro-algae (Undaria pinnatifida, Laminaria japonica, Enteromorpha intestinalis, and Gracilaria verrucosa) to cultivate Chlorella saccharophila. The growth of C. saccharophila was inhibited by 7 hydrolysates without active carbon treatment. In contrast, hydrolysates treated with active carbon increased the cell growth and product (oil and chlorophyll) formation by C. saccharophila. The oil contents of C. saccharophila treated with each hydrolysate were 41.26 ± 0.69% (glucose), 22.06 ± 1.21% (rapeseed straw), 28.65 ± 1.08% (barley straw), 31.15 ± 0.76% (rice straw), 31.50 ± 2.12% (U. pinnatifida), 31.49 ± 4.53% (L. japonica), 29.63 ± 3.93% (E. intestinalis), and 26.15 ± 1.99% (G. verrucosa), respectively. Lignocellulosics and marine macro-algae may be useful resources for improving the mass cultivation of C. saccharophila.
Abstract
In this study, we investigated the possibility of using hydrolysates of lignocellulosics (rapeseed straw, barley straw, rice straw) and marine macro-algae (Undaria pinnatifida, Laminaria japonica, Enteromorpha intestinalis, and Gracilaria verrucosa) to cultivate Chlorella saccharophila. The growth of C. saccharophila was inhibited by 7 hydrolysates without active carbon treatment. In contrast, hydrolysates treated with active carbon increased the cell growth and product (oil and chlorophyll) formation by C. saccharophila. The oil contents of C. saccharophila treated with each hydrolysate were 41.26 ± 0.69% (glucose), 22.06 ± 1.21% (rapeseed straw), 28.65 ± 1.08% (barley straw), 31.15 ± 0.76% (rice straw), 31.50 ± 2.12% (U. pinnatifida), 31.49 ± 4.53% (L. japonica), 29.63 ± 3.93% (E. intestinalis), and 26.15 ± 1.99% (G. verrucosa), respectively. Lignocellulosics and marine macro-algae may be useful resources for improving the mass cultivation of C. saccharophila.
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