돼지 유래 생체 조직의 이식재 활용을 위한 방사선 조사미생물 제어 평가
Comparison of Microbiological Safety of Porcine Grafts on Gamma Irradiation for Use of Xenografts
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This study was compared microbiological safety with gamma-irradiated porcinetendon and skin, as materials for the development of xenografts to regenerate damaged tissuesand protect secondary contamination. The porcine tendon and skin were gamma-irradiated afterinoculation of bacteria and virus to evaluate irradiation sensitivity of microorganisms. The resultshowed that the porcine tendon and skin were not different on the sensitivity of microorganismsby gamma irradiation. Bacteria inoculated in the porcine tendon and skin were confirmed that E. coli was the D10 values of 0.32±0.082 and 0.25±0.1 kGy on tendon and skin, and B. subtilis was4.00±0.312 and 3.88±0.3 kGy on gamma irradiation, respectively. Moreover, Virus inoculatedin the porcine tendon and skin was observed that poliovirus (PV) was 6.26±0.332 and 6.88±0.3kGy, and porcine parvovirus (PPV) was 1.75±0.131 and 1.73±0.2 kGy and bovine viraldiarrhoea virus (BVDV) was 3.70±0.212 and 3.81±0.2 kGy on gamma irradiation, respectively. Virus showed higher resistance compared to bacteria on gamma irradiation, but was not detectedCPE (cytopathic effect) by virus both tendon and skin at 25 kGy, a standard dose recommendedfrom IAEA for sterilization of medical products. Therefore, These results were considered thatgamma irradiation could control effectively bacteria and virus to develop safe porcine xenograft,and apply same irradiation doses to all tissues including tendon and skin of porcine.
Abstract
This study was compared microbiological safety with gamma-irradiated porcinetendon and skin, as materials for the development of xenografts to regenerate damaged tissuesand protect secondary contamination. The porcine tendon and skin were gamma-irradiated afterinoculation of bacteria and virus to evaluate irradiation sensitivity of microorganisms. The resultshowed that the porcine tendon and skin were not different on the sensitivity of microorganismsby gamma irradiation. Bacteria inoculated in the porcine tendon and skin were confirmed that E. coli was the D10 values of 0.32±0.082 and 0.25±0.1 kGy on tendon and skin, and B. subtilis was4.00±0.312 and 3.88±0.3 kGy on gamma irradiation, respectively. Moreover, Virus inoculatedin the porcine tendon and skin was observed that poliovirus (PV) was 6.26±0.332 and 6.88±0.3kGy, and porcine parvovirus (PPV) was 1.75±0.131 and 1.73±0.2 kGy and bovine viraldiarrhoea virus (BVDV) was 3.70±0.212 and 3.81±0.2 kGy on gamma irradiation, respectively. Virus showed higher resistance compared to bacteria on gamma irradiation, but was not detectedCPE (cytopathic effect) by virus both tendon and skin at 25 kGy, a standard dose recommendedfrom IAEA for sterilization of medical products. Therefore, These results were considered thatgamma irradiation could control effectively bacteria and virus to develop safe porcine xenograft,and apply same irradiation doses to all tissues including tendon and skin of porcine.
참고문헌 (13)
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