이종이식재 개발을 위한 감마선 조사 돼지 인대의미생물학적 안전성 및 인장강도
Microbiological Safety and Tensile Strength of Gamma Irradiated Porcine Tendon for the Development of Xenografts
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초록
This study was to determine the microbiological safety and tensile strength of gammairradiatedporcine tendon for the development of safe xenografts. Escherichia coli and Bacillus subtiliswere used as model pathogens and inoculated as 106~107 log colonies forming unit (CFU) g-1. As model virus from porcine, porcine parvovirus (PPV), bovine viral diarrhoea virus (BVDV) andpoliovirus were inoculated as 105~106 tissue culture infectious dose (TCID)50 g -1 into porcine skin. The D10 value of E. coli and B. subtilis was measured as 0.32±0.082 kGy and 4.0±0.312 kGy,respectively. Additionally, the D10 values of PPV, BVDV and poliovirus were also shown as 1.75±0.131 kGy, 3.70±0.212 kGy and 6.26±0.332 kGy, respectively. Gamma irradiation decreased thetensile strength of porcine tendon. Results indicate that microbiological safety of porcine tendoncan be improved significantly by gamma irradiation. However, further studies are needed toimprove the tensile strength of gamma-irradiated porcine tendon.
Abstract
This study was to determine the microbiological safety and tensile strength of gammairradiatedporcine tendon for the development of safe xenografts. Escherichia coli and Bacillus subtiliswere used as model pathogens and inoculated as 106~107 log colonies forming unit (CFU) g-1. As model virus from porcine, porcine parvovirus (PPV), bovine viral diarrhoea virus (BVDV) andpoliovirus were inoculated as 105~106 tissue culture infectious dose (TCID)50 g -1 into porcine skin. The D10 value of E. coli and B. subtilis was measured as 0.32±0.082 kGy and 4.0±0.312 kGy,respectively. Additionally, the D10 values of PPV, BVDV and poliovirus were also shown as 1.75±0.131 kGy, 3.70±0.212 kGy and 6.26±0.332 kGy, respectively. Gamma irradiation decreased thetensile strength of porcine tendon. Results indicate that microbiological safety of porcine tendoncan be improved significantly by gamma irradiation. However, further studies are needed toimprove the tensile strength of gamma-irradiated porcine tendon.
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