방사선 돌연변이 블랙베리 주성분 Cyanidin-3-glucoside의과산화수소 유발 산화적 손상에 대한 세포 보호 효과
Protective Effect of Cyanidin-3-glucoside, the Major Component of Rubus fruticosus L. Mutants by Irradiation, on H2O2-induced Oxidative Damage in HepG2 Cells
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This study was conducted to analyze the protective capacity of cyanidin-3-glucoside(C3G), which is rich in mulberry and blackberry as an anthocyanin pigment. In this study, we foundthat treatment with C3G significantly reduced ROS production in hydrogen peroxide (H2O2)-treated HepG2 cells in a dose-dependent manner. In addition, treatment with C3G significantlyincreased the cell viability in a dose-dependent manner in H2O2-treated HepG2 cells. Moreover,treatment with C3G dose-dependently decreased the release of LDH and activation of caspase-3 inHepG2 cells treated with H2O2. Furthermore, the DNA damage in H2O2-treated HepG2 cells wasdecreased by C3G treatment when compared with the control group in a dose-dependent manner. Additionally, treatment with C3G recovered the activity of antioxidant enzymes such as superoxidedismutase and catalase in H2O2-treated HepG2 cells. To summarize, these results suggestthat C3G protects cells from H2O2-induced oxidative damage by activating antioxidant enzymes.
Abstract
This study was conducted to analyze the protective capacity of cyanidin-3-glucoside(C3G), which is rich in mulberry and blackberry as an anthocyanin pigment. In this study, we foundthat treatment with C3G significantly reduced ROS production in hydrogen peroxide (H2O2)-treated HepG2 cells in a dose-dependent manner. In addition, treatment with C3G significantlyincreased the cell viability in a dose-dependent manner in H2O2-treated HepG2 cells. Moreover,treatment with C3G dose-dependently decreased the release of LDH and activation of caspase-3 inHepG2 cells treated with H2O2. Furthermore, the DNA damage in H2O2-treated HepG2 cells wasdecreased by C3G treatment when compared with the control group in a dose-dependent manner. Additionally, treatment with C3G recovered the activity of antioxidant enzymes such as superoxidedismutase and catalase in H2O2-treated HepG2 cells. To summarize, these results suggestthat C3G protects cells from H2O2-induced oxidative damage by activating antioxidant enzymes.
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