Antioxidant and Neuronal Cell Protective Effects of Aqueous Extracts from Lotus Leaf Tea
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Antioxidant and neuronal cell protective effects of aqueous extract from lotus (Nelumbo nucifera) leaf tea (LLTE) were investigated. The 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging effect, ferric reducing antioxidant power, and malondialdehyde inhibition of LLTE were increased in a dose dependent manner. Intracellular reactive oxygen species accumulation resulting from hydrogen peroxide (H2O2) treatment was significantly reduced when LLTE were present in the media compared to PC12 cells treated with H2O2 only. In neuronal cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- tetrazoliumbromide (MTT), LLTE showed protective effect against H2O2-induced neurotoxicity. In addition, lactate dehydrogenase release into medium was also inhibited by LLTE (7.13-43.89%). Total phenolics of LLTE were 33.16 mg/g and a quercetin was identified as major phenolics (105.93 mg/100g). Therefore, above these data suggest that LLTE including quercetin may be useful in the natural antioxidant substance, and may reduce the risk of neurodegenerative disease.
Abstract
Antioxidant and neuronal cell protective effects of aqueous extract from lotus (Nelumbo nucifera) leaf tea (LLTE) were investigated. The 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging effect, ferric reducing antioxidant power, and malondialdehyde inhibition of LLTE were increased in a dose dependent manner. Intracellular reactive oxygen species accumulation resulting from hydrogen peroxide (H2O2) treatment was significantly reduced when LLTE were present in the media compared to PC12 cells treated with H2O2 only. In neuronal cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- tetrazoliumbromide (MTT), LLTE showed protective effect against H2O2-induced neurotoxicity. In addition, lactate dehydrogenase release into medium was also inhibited by LLTE (7.13-43.89%). Total phenolics of LLTE were 33.16 mg/g and a quercetin was identified as major phenolics (105.93 mg/100g). Therefore, above these data suggest that LLTE including quercetin may be useful in the natural antioxidant substance, and may reduce the risk of neurodegenerative disease.
참고문헌 (36)
- Almajano, M. P, "Antioxidant and antimicrobial activities of tea infusions", Food Chem, 108, pp.55~63 (2008)
- Behl, C, "Glucocorticoids enhance oxidative stress-induced cell death in hippocampal neurons in vitro", Endocrinology, 138, pp.101~106 (1997)
- Behl, C, "Hydrogen peroxide mediates amyloid β protein toxicity", Cell, 77, pp.817~827 (1994)
- Chang, S. T, "Antioxidant activity of extracts from Acacia confuse bark and heartwood", J. Agr. Food Chem, 49, pp.3420~3424 (2001)
- Chung, K. T, "Tannins and human health: A review", Crit. Rev. Food Sci. Nutr, 38, pp.421~464 (1998)
- Crundman, M, "Antioxidant strategies for Alzheimer’s disease", Proc. Nutr. Soc, 61, pp.191~202 (2002)
- Dasgupta, N, "Antioxidant activity of Piper betle L. leaf extract in vitro", Food Chem, 88, pp.129~224 (2004)
- Dorman, H. J. D, "Fe(Ⅲ) reductive and free radical-scavenging properties of summer savory (Satureja hortensis L.) extract and subtractions", Food Chem, 88, pp.193~199 (2004)
- Dreosti, I. E, "Inhibition of carcinogenesis by tea: the evidence from experimental studies", Crit. Rev. Food Sci. Nutr, 37, pp.761~770 (1997)
- 구현률, "Simultaneous Determination of Quercetin and its Glycosides from the Leaves of Nelumbo nucifera by Reversed-Phase High-Performance Liquid Chromatography", Archives of Pharmacal Research, 32(2), pp.201~206 (2009), 대한약학회
- Ju Yeon Ha, "Cytoprotective Activity of Lotus (Nelumbo nucifera Gaertner) Leaf Extracts on the Mouse Embryonic Fibroblast Cell", Food Science and Biotechnology, 19(5), pp.1171~1176 (2010), 한국식품과학회
- Hagerman, A. E, "High molecular weight plant polyphenolics (tannins) as biological antioxidants", J. Agr. Food Chem, 46, pp.1887~1892 (1998)
- Heo, H. J, "Protective effect of quercetin and vitamin C against oxidative stressinduced neurodegeneration", J. Agr. Food Chem, 52, pp.7514~7517 (2004)
- Heo, H. J, "Protective effect of 4’,5-dihydroxy-3’,6,7- trimethoxyflavone from Artemisia asiatica against Aβ -induced oxidative stress in PC12 cells", Amyloid, 8, pp.194~201 (2001)
- Jankun, J, "Why drinking green tea could prevent cancer", Nature, 387, pp.561~ (1997)
- Jeong, C. H, "Antioxidant activities from the aerial parts of Platycodon grandiflorum", Food Chem, 118, pp.278~282 (2010)
- Kashiwada, Y, "Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera, and structure-activity correlations with related alkaloids", Bioor. Med. Chem, 13, pp.443~448 (2005)
- 김영준, "Sweet and sour cherry phenolics and their protective effects on neuronal cells", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 53, pp.9921~9927, American Chemical Society
- 이기원, "Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals", Journal of Agricultural and Food Chemistry, 50(13), pp.3713~3717, American Chemical Society
- Kou, M. C, "Cyphomandra betacea Sendt. phenolics protect LDL from oxidation and PC12 cells from oxidative stress", LWT-Food Sci. Technol, 42, pp.458~463 (2009)
- La, Cour B, "Traditional Chinese medicine in treatment of hyperlipidaemia", J. Ethnopharmacol, 46, pp.125~129 (1995)
- Mingyu Li, "Quercetin in a Lotus Leaves Extract May be Responsible for Antibacterial Activity", Archives of Pharmacal Research, 31(5), pp.640~644 (2008), 대한약학회
- Lin, H. Y, "Antioxidative effect and active components from leaves of lotus (Nelumbo nucifera)", J. Agr. Food Chem, 57, pp.6623~6629 (2008)
- Markesbery, W. R, "Oxidative alterations in Alzheimer’s disease", Brain Pathol, 9, pp.133~146 (1999)
- Ono, Y, "Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats", J. Ethnopharmacol, 106, pp.238~244 (2006)
- Ott, M, "Cytochrome c release from mitochondria proceeds by a two-step process", Proc. Natl. Acad. Sci. U.S.A, 99, pp.1259~1263 (2001)
- Ozsoy, N, "Antioxidant activity of Smilax excels L. leaf extracts", Food Chem, 110, pp.571~583 (2008)
- Salet, C, "Singlet oxygen produced by photodynamic action causes inactivation of the mitochondrial permeability transition pore", J. Biol. Chem, 272, pp.21938~21943 (1997)
- Sevanian, A, "Lipid peroxidation in membranes and low-density lipoproteins: similarities and differences", Free Radic. Biol. Med, 29, pp.306~311 (2000)
- Siddhuraju, P, "The antioxidant activity and free radical scavenging capacity of dietary phenol extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds", Food Chem, 105, pp.950~958 (2007)
- Silva, B. A, "Phytochemical and antioxidant characterization of Hypericum perforatum alcoholic extracts", Food Chem, 90, pp.157~167 (2005)
- Valencia, A, "Reactive oxygen species induce different cell death mechanisms in cultured neurons", Free Radic. Biol. Med, 36, pp.1112~1125 (2004)
- Wang, H, "Determination of flavonols in green and black tea leaves and green tea infusions by high-performance liquid chromatography", Food Res. Int, 34, pp.223~227 (2001)
- Wu, M. J, "Antioxidant activity of methanol extract of the lotus leaf (Nelumbo nucifera Gertn)", Am. J. Clin. Med, 31, pp.687~698 (2003)
- Youdim, K. A, "A possible emerging role of phytochemicals in improving age-related neurological dysfunctions: a multiplicity of effects", Free Radic. Biol. Med, 30, pp.583~594 (2001)
- Yu, D. H, "Protection of PC12 cells against superoxide-induced damage by isoflavonoids from Astragalus mongholicus", Biomed. Environ. Sci, 22, pp.50~54 (2009)
