본문 바로가기
KERD
논문

당귀, 작약, 지황, 천궁 혼합 발효물의 항산화, 항주름 및 미백 효과

Antioxidant, Anti-Wrinkle Activity and Whitening Effect of Fermented Mixture Extracts of Angelica gigas, Paeonia Lactiflora, Rehmannia chinensis and Cnidium officinale

강희철|엄지나|민진우|주광식
한국약용작물학회|2017.06|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제25권 제3호|pp. 152-159|DOI http://www.medicinalcrop.org

1

조회

0

다운로드

20

인용

0

참고문헌

키워드

SamultangAngelica gigasAntioxidant ActivityCnidium officinaleAnti-Wrinkle ActivityLeuconostoc mesenteroidesPaeonia LactifloraRehmannia chinensisWhitening Effect

초록

Background: In this study, examined the effects of an extract of a mixture of Angelica gigas, Cnidium officinale, Paeonia lactiflora, and Rehmannia glutinosa fermented by Leuconostoc mesenteroides, with enhanced value and functionality. In oriental medicine, a mixture of these herbs is called Samultang. Methods and Results: In this study, we evaluated the effects of a fermented extract of Samultang on oxidative stress, procollagen type I expression, and melanin production. Samultang was extracted with 70% ethanol, followed by inoculation with Leuconostoc mesenteroides to obtain the fermented extract. The evaluation of viability of B16F10 cells and human foreskin fibroblast (HHF) revealed that both ethanol and fermented extracts of Samultang were non-toxic. The results of 1,1-diphenyl-2-picrylhydrazyl (DPPH) test showed that the fermented extract of Samultang (SC50 value = 100 ㎍/㎖) was a more effective DPPH free radical scavenger than its ethanol extract. In addition, procollagen type I expression was higher in cells treated with the fermented extract of Samultang than in cells treated with ethanol. In the non-toxic concentration range, the fermented extract of Samultang showed strong inhibitory effect on melanin production in α-melanocyte stimulatin hormone-stimulated B16F10 cells (IC50 = 37.9 ㎍/㎖). Conclusions: These results suggest that the fermented extract of Samultang has considerable protential as a cosmetic ingredient owing to its antioxidant, anti-wrinkle, and whitening effects.

Abstract

Background: In this study, examined the effects of an extract of a mixture of Angelica gigas, Cnidium officinale, Paeonia lactiflora, and Rehmannia glutinosa fermented by Leuconostoc mesenteroides, with enhanced value and functionality. In oriental medicine, a mixture of these herbs is called Samultang. Methods and Results: In this study, we evaluated the effects of a fermented extract of Samultang on oxidative stress, procollagen type I expression, and melanin production. Samultang was extracted with 70% ethanol, followed by inoculation with Leuconostoc mesenteroides to obtain the fermented extract. The evaluation of viability of B16F10 cells and human foreskin fibroblast (HHF) revealed that both ethanol and fermented extracts of Samultang were non-toxic. The results of 1,1-diphenyl-2-picrylhydrazyl (DPPH) test showed that the fermented extract of Samultang (SC50 value = 100 ㎍/㎖) was a more effective DPPH free radical scavenger than its ethanol extract. In addition, procollagen type I expression was higher in cells treated with the fermented extract of Samultang than in cells treated with ethanol. In the non-toxic concentration range, the fermented extract of Samultang showed strong inhibitory effect on melanin production in α-melanocyte stimulatin hormone-stimulated B16F10 cells (IC50 = 37.9 ㎍/㎖). Conclusions: These results suggest that the fermented extract of Samultang has considerable protential as a cosmetic ingredient owing to its antioxidant, anti-wrinkle, and whitening effects.

참고문헌 (37)

  1. Ahn CK, "Sangyong hanbang haeseul-jip", pp.333~ (1999), Kihwa Pharmaceutical Co., Ltd
  2. Ahn HD, "Articles : Experimental study of Siwutang on reducing side–effects of the anticancer agents", Journal of Physiology and Pathology in Korean Medicine, 9, pp.341~359 (1995)
  3. Baumann L, "Cosmetic dermatology: Principles and practice", pp.10~ (2002), McGraw-Hill Co., Inc
  4. Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
  5. Brenneisen P, "Ultraviolet-B irradiation and matrix metalloproteinases: From induction via signaling to initial events", Annals of the New York Academy of Sciences, 973, pp.31~43 (2002)
  6. Chae SK, "Standard food analysis", pp.381~382 (2002), Jigu Publishing
  7. Chakraborty AK, "Effect of arbutin on melanogenic proteins in human melanocytes", Pigment Cell and Melanoma Research, 11, pp.206~212 (1998)
  8. 최화정, "Streptococcus thermophilus로 발효한 한약재 발효분말의 항염증 및 미백 효과", 대한화장품학회지, 41(2), pp.159~164 (2015), 대한화장품학회[DOI]
  9. 최우석, "유산균 발효를 통한 황금 추출물의 항염증 효과 증진", 대한화장품학회지, 39(4), pp.303~311 (2013), 대한화장품학회
  10. Duval B, "The stimulation of phenolics and antioxidant activity in pea(Pisum sativum)elicited by genetically transformed anise root extract", Journal of Food Biochemistry, 25, pp.361~377 (2001)
  11. Heo J, "Dongui bogam", pp.113~ (1976), Nam sandang
  12. 홍경표, "Leuconostoc mesenteroides KCTC3505를 이용한 발효자몽 추출물 생산 조건의 최적화", 동아시아식생활학회지, 21(5), pp.661~668 (2011), 동아시아식생활학회
  13. Hosoi J, "Regulation of melanin synthesis of B16 mouse melanoma cells by 1α, 25-dihydroxyvitamin D3 and retinoic acid", Cancer Research, 45, pp.1474~1478 (1985)
  14. Hubert J, "Effects of fermentation on the phytochemical composition and antioxidant properties of soy germ", Food Chemistry, 109, pp.709~721 (2008)
  15. 임도연, "Lactobacillus plantarum으로 발효시킨 황칠나무 잎 추출물의 피부 미백 관련 효과", 생약학회지, 47(1), pp.18~23 (2016), 한국생약학회
  16. Imokawa G, "A Possible mechanism underlying the ceramide deficiency in atopic dermatitis : Expression of a deacylase enzyme that cleaves the N-acyl linkage of sphingomyelin and glucosylceramide", Journal of Dermatological Science, 55, pp.1~9 (2009)
  17. Ito S, "Quantitative analysis of eumelanin and pheomelanin in humans, mice, and other animals : A comparative review", Pigment Cell and Melanoma Research, 16, pp.523~531 (2003)
  18. 전지민, "유산균 발효에 의한 인삼열매 추출물의 항산화 및 항노화 효과", 대한화장품학회지, 37(1), pp.75~81 (2011), 대한화장품학회
  19. 강창희, "갈대 발효추출물의 이화학적 특성 및 생리활성 연구", 생약학회지, 47(3), pp.273~279 (2016), 한국생약학회
  20. 김철희, "초고압 추출 공정에 의한 당귀 추출물의 미백 및 자외선 차단 효과", 한국약용작물학회지, 16(4), pp.255~260 (2008), 한국약용작물학회
  21. 김철중, "미생물 처리 발효 산삼배양근의 생리활성 변화", 한국약용작물학회지, 24(3), pp.191~197 (2016), 한국약용작물학회[DOI]
  22. 김용민, "어성초(Houttuynia cordata Thunb) 유산균 발효물의 항산화 활성", 생명과학회지, 26(4), pp.468~474 (2016), 한국생명과학회
  23. 김은경, "四物湯이 알러지 염증반응에 미치는 영향", 동의생리병리학회지, 21(3), pp.617~625 (2007), 한의병리학회
  24. Kim HJ, "Effects of a constituent herbs of Samul–tang on anti–fatigue", The Journal of Oriental Obstetrics and Gynecology, 13, pp.54~93 (2000)
  25. 김민정, "숙성된 3종 인삼추출물의 항산화 및 주름개선 효과", 대한화장품학회지, 38(2), pp.155~162 (2012), 대한화장품학회
  26. 김수은, "Hairless 마우스에서 천궁 열수추출물의 피부장벽 및 염증 완화 효과", 대한미용학회지, 7(2), pp.137~145 (2011), 대한미용학회
  27. 이호영, "사물탕, 사군자탕, 팔물탕, 십전대보탕의 약리 활성 비교 연구", 동의신경정신과학회지, 21(4), pp.41~51 (2010), 대한한방신경정신과학회
  28. Lee JS, "Experimental studies on the effect of Samul-tang and Samul-tang gagambang aquaacupuncture", The Journal of Oriental Obstetrics and Gynecology, 14, pp.1~26 (2001)
  29. 이미경, "만형자 추출물의 항산화 및 주름개선 효과", Journal of Applied Biological Chemistry, 58(2), pp.125~129 (2015), 한국응용생명화학회
  30. McCord JM, "Oxygen-derived radicals: A link between reperfusion injury and inflammation", Federation Proceedings, 46, pp.2402~2406 (1987)
  31. 오영지, "천궁(Cnidium officinale Makino) 지상부 추출물의 항산화 활성 평가", 한국약용작물학회지, 18(6), pp.373~378 (2010), 한국약용작물학회
  32. Ranke J, "Biological effects of imidazolium ionic liquids with varying chain lengths in acute Vibrio fischeri and WST-1 cell viability assays", Ecotoxicology and Environmental Safety, 58, pp.396~404 (2004)
  33. Tahara E, "Effect of Shimotsu-to(a kampo medicine, Si-wu-tang)and its constituents on triphasic skin reaction in passively sensitized mice", Journal of Ethnopharmacology, 68, pp.219~228 (1999)
  34. Taga MS, "Chia seeds as a source of natural lipid antioxidants", Journal of the American Oil Chemists’ Society, 61, pp.928~931 (1984)
  35. Torel J, "Antioxidant activity of flavonoids and reactivity with peroxy radical", Phytochemistry, 25, pp.383~385 (1986)
  36. 양현주, "황련해독탕 및 발효황련해독탕의 항염증 효과", 한방안이비인후피부과학회지, 24(2), pp.1~15 (2011), 대한한방안이비인후피부과학회
  37. 유보람, "품종별 지황의 Catalpol 함량 및 항산화능", 한국식품영양과학회지, 40(4), pp.481~485 (2011), 한국식품영양과학회

관련 논문AI추천

저온숙성 복합한약재 추출물의 항산화능, 항균활성 및 NO 생성 억제능김미경대한미용학회 · 대한미용학회지2020 · 제16권 제4호유산균 발효 배초향 추출물의 항산화 활성과 주름개선 효능김남영, 박대수, 이현용한국약용작물학회 · 한국약용작물학회지2015 · 제23권 제1호화장품 소재로서 더덕과 발효더덕 추출물의 피부관련생리활성 비교탐색강은주, 김미애, 모정희한국인체미용예술학회 · 한국인체미용예술학회지2013 · 제14권 제4호건지황 추출물의 피부노화억제 효능에 대한 연구강상진, 정문신한국화장품미용학회 · 한국화장품미용학회지2017 · 제7권 제2호참당귀 세미 추출물의 미백 및 항산화 효과허목, 구성철, 문윤호, 박우태, 박정용, 서경혜, 이상훈, 이윤정, 장재기, 최장남, 허윤찬한국자원식물학회 · 한국자원식물학회지2021 · 제34권 제1호Antioxidant and anti-wrinkle effects of fermented Houttuynia cordata extract김범식, 박미혜한국식품저장유통학회 · Food Science and Preservation2025 · 제32권 제2호

이 간행물의 다른 논문 (5건)

서양금혼초 부위별 추출물의 티로신에이즈, α-글루코시다아제 저해활성 및 항산화효과김주성, 송선경, 엄태길, 고현민, 조가영pp. 139-145지황 품종별 뿌리에서 Iridoid 배당체와 GABA 분석김연복, 박춘근, 장재기, 이상훈, 윤정수, 김재광pp. 146-151대추나무의 단근처리가 생육 및 결실에 미치는 영향홍의연, 이경희, 김상희, 이성균, 오하경, 김충우, 이종원pp. 160-164Therapeutic Potential of Chinese Prescription Hachimi-Jio-Gan and Its Crude Drug Corni Fructus against Diabetic Nephropathy최재수, Takako Yokozawa, 박찬흠pp. 165-174인삼 수경재배 시 붕소와 철 과잉 농도가 인삼의 생리장해 증상, 생육 및 무기원소 흡수에 미치는 영향김기홍, 박기춘, 강수현, 박홍우, 장인배, 장인복, 서태철, 유진, 이응호, 서수정pp. 175-182
원문 보기