배양 인체 피부 흑색종세포에 대한 제라늄오일의 항산화 활성 및 멜라닌 합성에 미치는 영향
Effects of Geranium Oil on Antioxidative Activity and Melanin Synthesis in Cultured Human Skin Melanoma Cell
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초록
For the purpose of searching for whitening resources using essential oil of herb, the antioxidative activity and melanogenesis of Geranium (Palargonium graveolens) oil were examined in cultured human skin melanoma cells (SK-MEL-3). the cell viability, superoxide dismutase (SOD)-like activity, inhibitory activity of lipid peroxidation, tyrosinase activity and total amount of melanin were measured by colorimetric assay. For this experiment, SK-MEL-3 cells were treated with the media containing 25~55 μU/mL, glucose oxidase (GO) for 4 hours, and assessed for the cytotoxicity. GO significantly decreased cell viability compared with control and XTT_50 value was determined at 45 μU/mL of GO. In the antioxidative activity of Geranium oil, it showed SOD-like activity and the inhibitory activity of lipid peroxidation. While, in the melanogenesis of Geranium oil, Geranium oil significantly decreased the tyrosinase activity and total amount of melanin which were increased by GO-induced cytotoxicity. From these results, it is suggested that Geranium oil was effective in the protection of GO-induced cytotoxicity by antioxidative activity and inhibitory effect of melanogenesis. Finally, an essential oil such as Geranium oil may be a putative agent for the improvement of whitening effect.
Abstract
For the purpose of searching for whitening resources using essential oil of herb, the antioxidative activity and melanogenesis of Geranium (Palargonium graveolens) oil were examined in cultured human skin melanoma cells (SK-MEL-3). the cell viability, superoxide dismutase (SOD)-like activity, inhibitory activity of lipid peroxidation, tyrosinase activity and total amount of melanin were measured by colorimetric assay. For this experiment, SK-MEL-3 cells were treated with the media containing 25~55 μU/mL, glucose oxidase (GO) for 4 hours, and assessed for the cytotoxicity. GO significantly decreased cell viability compared with control and XTT_50 value was determined at 45 μU/mL of GO. In the antioxidative activity of Geranium oil, it showed SOD-like activity and the inhibitory activity of lipid peroxidation. While, in the melanogenesis of Geranium oil, Geranium oil significantly decreased the tyrosinase activity and total amount of melanin which were increased by GO-induced cytotoxicity. From these results, it is suggested that Geranium oil was effective in the protection of GO-induced cytotoxicity by antioxidative activity and inhibitory effect of melanogenesis. Finally, an essential oil such as Geranium oil may be a putative agent for the improvement of whitening effect.
참고문헌 (35)
- Anderson C, "Evaluation of massage with essential oils on childhood atopic eczema", Phytother Res, 14(6), pp.452~456 (2000)
- Atsumi T, "Smelling lavender and rosemary increases free radical scavenging activity and decreases cortisol level in saliva", Psych Res, 150(1), pp.89~96 (2007)
- Borenfreund E, "Comparisons of two in vitro cytotoxicity assay. The neutral red (NR) and tetrazolium MTT tests", Toxicol In Vitro, 2, pp.1~8 (1988)
- Buckle J, "The role of aromatherapy in nursing care", Nurs Clin Nor Am, 36(1), pp.57~72 (2001)
- Cavanagh HMA, "Biological Activities of Lavender Essential Oil", Phytother Res, 16(4), pp.301~308 (2002)
- Choi BW, "Screening of the tyrosinase inhibitors from marine algae and medicinal plants", Kor J Pharmacogn, 29, pp.237~242 (1988)
- Chun-Whan Choi, "Antioxidant Constituents and a New Triterpenoid Glycoside from Flos Lonicerae", Archives of Pharmacal Research, 30(1), pp.1~7 (2007), 대한약학회
- Fujisawa S, "Anti-and pro-oxidant effects of oxidized quercetin, curcumin-related compounds with thiols or ascorbate as measured by the induction priod method", In Vivo, 20(1), pp.39~44 (2006)
- Gates MA, "A prospective study of dietary flavonoid intake and incidence of epithelial ovarian cancer", Int J cancer, 121(10), pp.2225~2232 (2007)
- 한승호, "국내 자생 식물 추출물의 항산화 활성 및 항균효과", 한국약용작물학회지, 14(1), pp.49~55 (2006), 한국약용작물학회
- Hosoi J, "Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid", Cancer Res, 45, pp.1474~1478 (1985)
- Husdon R, "The value of lavender for rest and activity in the elderly patient", Compl Ther Med, 4, pp.52~57 (1996)
- Itai T, "Psychological effects of aromatherapy on chronic hemodiallsis patients", Psychiatry Clin Neurosci, 54(4), pp.393~397 (2000)
- Jesberger JA, "Oxygen free radicals and brain dysfunction", Int J Neurosci, 57, pp.1~17 (1991)
- Kikuzaki H, "Antioxidant effects of some ginger constitients", J Food Sci, 58, pp.1407~1410 (1993)
- Kim HM, "Lavender oil inhibits intermediate-type Allergic reaction in mice and rats", British J Pharmacol, 51, pp.221~226 (1999)
- Koiso I, "Development of Recent Melanin Control Agents and Whitening Cosmetics", Fragrance J, 18(6), pp.39~41 (1990)
- Kwada M, "Anti-tumor effect of gallic acid on IL-2 lung cancer cells transplanted in mice", Anticancer Drugs, 12(10), pp.847~852 (2001)
- Lee JK, "1,8-Dihydroxynaphthalene(DHN)- melanin biosynthesis inhibitors increase erythritol production in Toula corallina, and DHN-melanin inhibits erythrose reductase", Appl Environ Microbiol, 69, pp.3427~3434 (2003)
- Lerner AB, "Biochemistry of melanin formation", Physiol Rev, 30, pp.92~126 (1984)
- Leung HW, "Kaempferol induces apoptosis in human lung non-small carcinoma cells accompanied by an induction of antioxidant enzymes", Food Chem Toxicol, 45(10), pp.2005~2013 (2007)
- Li YL, "A flavonoid glycoside isolated from Smilax china L. rhizome in vitro anticancer cell lines", J Ethnopharmacol, 113(1), pp.115~124 (2007)
- Marklund S, "Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur J Biochem, 47, pp.468~474 (1974)
- Michikawa M, "Oxygen radical induced neurotoxicity in spinal cord neuron cultures", J Neurosci Res, 37, pp.62~70 (1994)
- Oh YL, "Effect of Oenothera lamarrckiana Extract Belong in Oenotherceae on Antioxidant Activity and Melanogenesis", J Korean Soc People Plants Environ, 14(1), pp.9~15 (2001)
- Pannala AS, "Inhibition of peroxynitrite dependent tyrosine nitration by hydroxycinnamates: nitration or electron donation", Free Radic Biol Med, 24, pp.594~606 (1998)
- Park ST, "Methylmercury-induced neurotoxicity in cerebral neuron culture is blocked by anti-oxidants and NMDA receptor antagonists", Neurotoxicol, 17, pp.37~49 (1996)
- Parviz O, "Antistreptococcal and Antioxidant Activity of Essential Oil from Matricarea chamomile L", Biol Sci, 2(2), pp.155~160 (2007)
- Rosen D, "Mutation in Cu", Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis, 1993, pp.362, 59~62
- Sankar D, "Effect of Sesame Oil on Diuretics or β-blocker in the modulation of Blood Pessure, Anthropometry, Lipid Profile, and Redox status", Yale J Biol Med, 79(1), pp.19~26 (2006)
- Tiziana BM, "Antimicrobial and antioxidant properties of some comercial essential oils", Rlavour Fragr J, 13, pp.235~244 (1998)
- Wang L, "Distinctive antioxidant and antiinflammatory effects of flavonols", J Agric Food Chem, 54(26), pp.9798~9804 (2006)
- Warner BB, "Free radical-mediated disease in pediatrics", Perinatol Seminar, 16, pp.47~57 (1990)
- Witting LA, "Vitamin E and lipid antioxidants in free-radical initiated reactions, in Free Radicals in Biology", pp.295~319 (1980), Acad Press
- Zullyt B, "Antioxidant Mechanism is involved in the Gastroprotective Effects of Ozonized Sunflower Oil in Ethanol-Induced Ulcers in Rats", Mediators Inflamm, pp.658~673 (2007)