제라늄(Geranium) Essential oil이 B16F10 Melanoma cell에서 melanin 합성에 미치는 향
Inhibitory Effect of Geranium oil on Melanin Synthesis in B16F10 Mouse Melanoma Cells
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With the extension of life expectancy and the improvement of living standards, people's desire for a healthy and beautiful life has continuously been increased. Furthermore, as the destruction of the ozone layer due to environmental pollution is being accelerated, interest in the method of protecting the skin from external stimuli such as ultraviolet light is increasing regardless of sex and age. Accordingly, many studies have been conducted worldwide to find whitening functional materials from natural plants that are reliable for their stability. In this study, we investigated inhibitory effect of a herbal essential oil, Geranium, in α-MSH-induced melanogenesis in B16F10 melanoma cells, to determine applicability of this material as the functional ingredient for skin whitening cosmetics. As a result of in vitro tyrosinase activity assay, geranium oil showed significant inhibition of the enzyme activity for L-tyrosin hydroxylation and L-DOPA oxidation. Especially in B16F10 cells, we found that pre-treatment of geranium essential oil efficiently prevented extracellular secretion of melanin with dose-dependent manner, which was much effective than those of in vitro tyrosinase activity assay. Therefore, it suggests that maybe various components contained in the geranium essential oil affect multiple factors involved in α-MSH-induced melanogenesis in B16F10 cells, which inhance the value of geranium as a effective functional ingredient of whitening cosmetics.
Abstract
With the extension of life expectancy and the improvement of living standards, people's desire for a healthy and beautiful life has continuously been increased. Furthermore, as the destruction of the ozone layer due to environmental pollution is being accelerated, interest in the method of protecting the skin from external stimuli such as ultraviolet light is increasing regardless of sex and age. Accordingly, many studies have been conducted worldwide to find whitening functional materials from natural plants that are reliable for their stability. In this study, we investigated inhibitory effect of a herbal essential oil, Geranium, in α-MSH-induced melanogenesis in B16F10 melanoma cells, to determine applicability of this material as the functional ingredient for skin whitening cosmetics. As a result of in vitro tyrosinase activity assay, geranium oil showed significant inhibition of the enzyme activity for L-tyrosin hydroxylation and L-DOPA oxidation. Especially in B16F10 cells, we found that pre-treatment of geranium essential oil efficiently prevented extracellular secretion of melanin with dose-dependent manner, which was much effective than those of in vitro tyrosinase activity assay. Therefore, it suggests that maybe various components contained in the geranium essential oil affect multiple factors involved in α-MSH-induced melanogenesis in B16F10 cells, which inhance the value of geranium as a effective functional ingredient of whitening cosmetics.
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