돼지감자 추출물을 활용한 이너뷰티 천연소재 개발 및 기능성 평가
Inner Beauty Material Development and Functional Evaluation using Helianthus tuberosus Extracts
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
The purpose of this study was to determine the potential of Helianthus tuberosus as a natural material for use in development of cosmetics and products for enhancement of inner beauty by examining the bioactivity of water extract (HTW) and 20% ethanol extract (HTE). More than 47% protective effects against oxidative stress were obtained in HepG2 cell lines by treatment with 400 μg/mL of HTW and HTE. Antibacterial activity increased as the treatment concentration of each extract of H. tuberosus extract increased. None of the extracts exhibited toxicity in a RAW 264.7 cell line in 150 μg/mL. Reduced production of NO to 69.9±1.9% was observed for HTW and 69.7±1.5% for HTE at a concentration of 100 μg/mL. Concentration-dependent inhibition of production of inflammatory cytokines, TNF-α, IL-1β, and IL-6, was observed. Cytoxicity of H. tuberosus extract was not detected in Caco-2 cells and concentration-dependent inhibition of NO production was observed. Activity of α-amylase and protease enzyme, which are digestive enzymes, increased with increasing concentration of H. tuberosus extract. The findings of this study will provide basic data for development of a natural material for utilization in development of cosmetics and products for enhancement of inner beauty using H. tuberosus.
Abstract
The purpose of this study was to determine the potential of Helianthus tuberosus as a natural material for use in development of cosmetics and products for enhancement of inner beauty by examining the bioactivity of water extract (HTW) and 20% ethanol extract (HTE). More than 47% protective effects against oxidative stress were obtained in HepG2 cell lines by treatment with 400 μg/mL of HTW and HTE. Antibacterial activity increased as the treatment concentration of each extract of H. tuberosus extract increased. None of the extracts exhibited toxicity in a RAW 264.7 cell line in 150 μg/mL. Reduced production of NO to 69.9±1.9% was observed for HTW and 69.7±1.5% for HTE at a concentration of 100 μg/mL. Concentration-dependent inhibition of production of inflammatory cytokines, TNF-α, IL-1β, and IL-6, was observed. Cytoxicity of H. tuberosus extract was not detected in Caco-2 cells and concentration-dependent inhibition of NO production was observed. Activity of α-amylase and protease enzyme, which are digestive enzymes, increased with increasing concentration of H. tuberosus extract. The findings of this study will provide basic data for development of a natural material for utilization in development of cosmetics and products for enhancement of inner beauty using H. tuberosus.
참고문헌 (71)
- Akbar A, "Synthesis and antimicrobial activity of zinc oxide nanoparticles against foodborne pathogens Salmonella typhimurium and Staphylococcus aureus", Biocatal Agric Biotechnol, 17, pp.36~42 (2019)
- Alley MC, "Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay", Cancer Res, 48, pp.589~601 (1988)
- 방극수, "LED광원에 따른 감초 지상부의 생리활성 및 성분 평가", 한국자원식물학회지, 28(1), pp.126~134 (2015), 한국자원식물학회[DOI]
- Berg R, "Applicability of an improved trolox equivalent antioxidant capacity(TEAC)assay for evaluation of antioxidant capacity measurements of mixtures", Food Chem, 66, pp.511~517 (1999)
- Bernfeld P, "Amylases α and β methods in Enzymology", Methods Enzymol, 1, pp.149~158 (1955)
- Boligon AA, "Technical evaluation of antioxidant activity", Med Chem, 4, pp.517~522 (2014)
- Carabin IG, "Evaluation of safety of inulin and oligofructose as dietary fiber", Regul Toxicol Pharmacol, 30, pp.268~282 (1999)
- Chang J, "Cytotoxic terpenoid and immunosuppressive phenolic glycosides from the root bark of Dictamnus dasycarpus", Planta Med, 68, pp.425~429 (2002)
- Chiou WF, "Camptothecin suppresses nitric oxide biosynthesis in RAW 264. 7 macrophages", Life Sci, 69, pp.625~635 (2001)
- 조은지, "그라비올라(Annona muricata) 잎 에탄올 추출물의 항염증 효과", 한국식품영양과학회지, 46(6), pp.681~687 (2017), 한국식품영양과학회
- 최문희, "이너뷰티 제품 개발을 중심으로 국내산 대나무 줄기의 생물공학적 활용방안", KSBB Journal, 32(1), pp.1~8 (2017), 한국생물공학회[DOI]
- 최유나, "익모초 에탄올 추출물의 항산화 및 항염증 활성", 한국유기농업학회지, 28(4), pp.659~677 (2020), 한국유기농업학회
- Chung HT, "Nitric oxide as a bioregulator of apoptosis", Biochem Biophys Res Commun, 282, pp.1075~1079 (2001)
- nitrate in biological fluids, "Anal Biochem", 1982, 131~138
- Halliwell B, "Measuring reactive speciesand oxidative damage in vivo and in cell culture: howshould you do it and what do the results mean?", Br J Pharmacol, 142, pp.231~255 (2004)
- 한의정, "살균다시마 물 추출물 및 효소 추출물의 항산화활성", Journal of Chitin and Chitosan, 21(3), pp.207~214 (2016), 한국키틴키토산학회[DOI]
- 홍창의, "대추 추출물의 항산화 및 아토피 피부염 관련 항염증 효과", 생약학회지, 50(2), pp.86~95 (2019), 한국생약학회
- 임도연, "HPLC와 DPPH radical 소거능 측정 방법의 결합에 의한 약용 식물 추출물의 항산화 활성 비교", 생명과학회지, 27(1), pp.44~49 (2017), 한국생명과학회
- 장시성, "로스팅 녹두를 이용한 다식 제조 및 품질특성", 한국생활과학회지, 23(2), pp.357~366 (2014), 한국생활과학회
- 제재영, "Chitosan-phytochemical Conjugates의 항산화 및 항염증 활성", Journal of Chitin and Chitosan, 20(4), pp.245~250 (2015), 한국키틴키토산학회[DOI]
- 정상철, "천연소재로부터 피부효능을 갖는 피부미용식품의 개발동향", 아시안뷰티화장품학술지, 11(2), pp.203~212 (2013), 한국피부과학연구원
- Joo T, "Inhibition of nitric oxide production in LPS-stimulated RAW 264. 7 cells by stem bark of Ulmus pumila L", Saudi J Biol Sci, 21, pp.427~435 (2014)
- 정슬아, "감태 물 추출물의 Trypsin 저해활성에 대한 열 및 pH 안정성", 한국식품영양과학회지, 41(6), pp.840~845 (2012), 한국식품영양과학회[DOI]
- Jung YJ, "Inhibitory effect of methyl 2-(4'-methoxy-4'-oxobutanamide)benzoate from Jerusalem artichoke(Helianthus tuberosus)on the inflammatory paracrine loop between macrophages and adipocytes", J Agric Food Chem, 64, pp.9317~9325 (2016)
- 강보경, "Lipopolysaccharide로 유도된 RAW 264.7 세포에서 다시마 뿌리에탄올 추출물의 항염증 효과", 한국식품과학회지, 46(6), pp.729~733 (2014), 한국식품과학회[DOI]
- 강재란, "용매별 초석잠 추출물의 항산화 및 항당뇨 활성", 한국식품저장유통학회지, 24(5), pp.615~622 (2017), 한국식품저장유통학회
- Kawamata H, "Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated Raw 264. 7 cells a murine macrophage cell line", Am J Chin Med, 28, pp.217~226 (2000)
- 김대용, "각질세포에서 백작약 추출물의 항염증 효과", 대한미용학회지, 15(3), pp.247~254 (2019), 대한미용학회[DOI]
- 김은진, "자생식물과 생약자원 추출물의 폴리페놀, 플라보노이드 함량 및 항산화 활성 탐색", 한국식품과학회지, 44(3), pp.337~342 (2012), 한국식품과학회[DOI]
- 김준형, "돼지감자꽃의 예쁜꼬마선충 내의 항산화 효과", 생약학회지, 50(2), pp.96~101 (2019), 한국생약학회
- 김정란, "HIT-T15 세포에서 돼지감자 추출물의 항당뇨 효과", 한국식품영양과학회지, 39(1), pp.31~35 (2010), 한국식품영양과학회
- 김미현, "국내산 무화과에서 추출한 protease 조효소액의 안정성과 최적화에 관한 연구", 한국식품조리과학회지, 27(3), pp.29~37 (2011), 한국식품조리과학회
- 김민정, "In Vitro 항산화능 측정법에 대한 특징 분석과 채소․과일 시료에 대한 적용 사례 고찰", 한국식품영양과학회지, 40(7), pp.1053~1062 (2011), 한국식품영양과학회
- 김미경, "망고 과육 및 껍질 추출물의 항산화 및 항균 활성 비교", 대한미용학회지, 13(2), pp.113~118 (2017), 대한미용학회[DOI]
- 김미경, "대식세포에서 감태 에탄올 추출물의 NF-κB", MAPK 신호전달 억제를 통한 항염증 효과, 2016, pp.21(4), 236~241[DOI]
- 김상준, "오가나무 잎, 줄기의 항산화 및 항당뇨 효능 분석", 생약학회지, 50(2), pp.141~147 (2019), 한국생약학회
- Kim SK, "Schizandrin, oil compounds, and their extraction yield in fruits of Schisandra chinensis Baillon", Korean J Crop Sci, 45, pp.158~162 (2000)
- 김연숙, "돼지감자잎 추출물의 항산화 활성 및 간세포 보호 효과", 한국식품영양과학회지, 40(11), pp.1525~1531 (2011), 한국식품영양과학회
- 이보배, "제비꽃 추출물의 항산화 활성 및 α-Amylase와 α-Glucosidase에 대한 저해 활성", 한국식품영양과학회지, 37(4), pp.405~409 (2008), 한국식품영양과학회
- 이환, "마행감석탕 용매별 추출물의 항산화, 항염증 및 뇌세포보호 효과 비교", 생약학회지, 51(3), pp.163~170 (2020), 한국생약학회
- 이세원, "기능성 화장품 및 이너뷰티 소재 개발을 위한 표고버섯 물 추출물의 생리활성", 대한미용학회지, 17(4), pp.381~391 (2021), 대한미용학회[DOI]
- 이양숙, "금은화, 백선피, 연교 추출물의 항노화, 항산화 및 항염증 활성", 대한미용학회지, 16(1), pp.1~10 (2020), 대한미용학회[DOI]
- 이양숙, "구릿대 잎 추출물의 항산화 효과 및 생리활성", 한국식품저장유통학회지, 14(1), pp.78~86 (2007), 한국식품저장유통학회
- Lehrer RI, "Ultrasensitive assays for endogenous antimicrobial polypeptides", J Immunol Methods, 137, pp.167~173 (1991)
- 임정묵, "천연 화장품 소재 개발을 위한 산초 성분분석 및 생리활성 평가", 대한미용학회지, 16(3), pp.183~196 (2020), 대한미용학회[DOI]
- Liu RH, "Potential synergy of pytochemicals in cancer prevention: mechanism of action", J Nutr, 134, pp.3479S~3485S (2004)
- Liu S, "Assessments of antioxidant effect of black tea extract and its rationals by erythrocyte haemolysis assay, plasma oxidation assay and cellular antioxidant activity(CAA)assay", J Funct Foods, 18, pp.1095~1105 (2015)
- Mannick EE, "Inducible nitric oxide synthase, nitrotyrosine, and apoptosis in Helicobacter pylori gastritis: effect of antibiotics and antioxidants", Cancer Res, 56, pp.3238~3243 (1996)
- 민지영, "續斷의 RAW264.7 세포에서 LPS에 의해 유도되는 염증반응에 대한 효과", 대한본초학회지, 24(4), pp.189~195 (2009), 대한본초학회
- Moreno M, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", J Ethnopharmacol, 71, pp.109~114 (2000)
- 남궁승, "까마귀쪽나무 열매와 잎의 항염증 효과 비교 연구", 한국자원식물학회지, 28(2), pp.145~152 (2015), 한국자원식물학회[DOI]
- Osuagwu SO, "Antibacterial activity and phytochemical properties of selected medicinal plants against Salmonella typhi, Salmonella paratyphi A, B and C, clinical isolates in Lafia, Nigeria", Am Sci Res J Eng Technol Sci, 81, pp.203~221 (2021)
- 박병배, "돼지감자 분말 첨가 발효유의 이화학적 특성", Journal of Dairy Science and Biotechnology, 37(3), pp.196~205 (2019), 한국낙농식품응용생물학회[DOI]
- 박종철, "LPS유도 대식세포에서 Kaempferol-7-O-b-D-glucoside의 NO, PGE2 및 염증성 사이토카인 생성 저해 효과", 생약학회지, 47(4), pp.295~300 (2016), 한국생약학회
- 박정섭, "기장의 항산화 활성 및 항돌연변이 효능", 한국식품저장유통학회지, 27(2), pp.261~269 (2020), 한국식품저장유통학회
- 박정숙, "백선피의 iNOS발현과 염증성사이토카인의 생성에 미치는 영향", 생약학회지, 42(4), pp.348~353 (2011), 한국생약학회
- 박성철, "LPS로 활성화된 RAW 264.7 대식세포에서 애기땅빈대 (Euphorbia supina Rafin)의 염증매개물질 억제효과", 한국식품영양과학회지, 40(4), pp.486~492 (2011), 한국식품영양과학회
- Radi R, "Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide", J Biol Chem, 266, pp.4244~4250 (1991)
- Rahbari KM, "A Streptococcus quorum sensing system enables suppression of innate immunity", Am Soc Microbiol, 12, pp.e03400~20 (2021)
- Romier-Crouzet B, "Inhibition of inflammatory mediators by polyphenolic plant extracts in human intestinal Caco-2 cells", Food Chem Toxicol, 47, pp.1221~1230 (2009)
- 손동화, "홍삼에 의한 Caco-2 단세포층 간극의 안정화", 한국식품과학회지, 42(3), pp.335~342 (2010), 한국식품과학회
- Singleton VL, "Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents", Am J Enol Vitic, 16, pp.144~158 (1965)
- Soory M, "Relevance of nutritional antioxidant in metabolic syndrome, ageing and cancer : potential for therapeutic targeting", Infect Disord Drug Targets, 9, pp.400~414 (2009)
- Takarada K, "A comparison of the antibacterial efficacies of essential oils against oral pathogens", Oral Microbiol Immunol, 9, pp.61~65 (2004)
- Walia N, "Pea protein based vitamin D nanoemulsions: Fabrication, stability and in vitro study using Caco-2 cells", Food Chem, 305, pp.125475~ (2020)
- Wang KJ, "Antioxidant phenolic compounds from rhizomes of Polygonum paleaceum", J Ethnopharmacol, 96, pp.483~487 (2005)
- Wolfe KL, "Structure-activity relationships of flavonoids in the cellular antioxidant activity assay", J Agric Food Chem, 56, pp.8404~8411 (2008)
- Yang HS, "Laminaria japonica extract enhances intestinal barrier function by altering inflammatory response and tight junction-related protein in lipopolysaccharide-stimulated Caco-2 cells", Nutrients, 11, pp.1001~ (2019)
- 이양숙, "이팝나무 꽃 추출물의 항산화 및 항노화 활성", 대한미용학회지, 15(3), pp.229~237 (2019), 대한미용학회[DOI]
- 윤영한, "Enhancing the Effect of Aronia Extract on Hyaluronic Acid Synthesis through Liposome Formation", 인간식물환경학회지, 23(4), pp.465~473 (2020), 인간식물환경학회
- Yuan X, "Free radical scavenging activities and bioactive substances of Jerusalem artichoke(Helianthus tuberosus L. )leaves", Food Chem, 133, pp.10~14 (2012)
