쇠뜨기 (Equisetum arvense L.) 생식줄기와 영양줄기 열수 추출물의 항산화 및 항노화 활성
Anti-oxidative and Anti-aging Properties of Hot Water Extracts from Fertile and Sterile E. arvense Stems
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식용 및 한방생약재로 사용하는 쇠뜨기 (E. arvense) 생식줄 기와 영양줄기 열수 추출물의 항산화 및 항노화 활성을 측정 한 결과, 생식줄기는 폴리페놀 27.06 mg/g, 플라보노이드 45.62 mg/g으로 영양줄기보다 많았으며, 영양줄기는 191.14 mg/g의 환원당과 56.14 mg/g의 수용성 단백질을 함유하여 생식줄기 보다 2배 이상 많이 함유하였다. 아질산염 소거능은 pH 1.2의 2.0 mg/mL에서 생식줄기 92.24%로 천연 항산화제인 L-ascor- bic acid (92.45%)와 비슷한 소거 효과를 보였으며, 영양줄기는 81.65%의 소거능을 나타내었다. 2.0 mg/mL의 농도에서 생식줄 기는 24.77%의 SOD 유사활성과 61.48%의 XO 저해, 28.16% 의 tyrosinase 저해 효과를 보였으며, 영양줄기는 각각 16.58%, 25.57%, 52.88% 저해 효과를 보였다. Collagenase 저해는 생식 줄기 29.68%, 영양줄기 37.47%였으며, elastase 저해는 생식줄 기 33.33%, 영양줄기 27.12%였다. DPPH radical 소거는 0.05 mg/mL에서 생식줄기 (44.94%)와 영양줄기 (40.82%)가 L-ascor- bic acid (36.95%)보다 높은 활성을 보였고, 0.5 mg/mL에서는 모 두 90% 이상의 소거율을 나타냈으며 1.0 mg/mL 이상의 농도에 서는 L-ascorbic acid와 쇠뜨기 열수 추출물 간에 유의적인 차이 가 없었다. 이상의 결과, 쇠뜨기 생식줄기는 영양줄기보다 아질산염 소 거 및 SOD 유사활성이 높았고, XO, tyrosinase 및 elastase 저해 효과가 높았으며, 영양줄기는 생식줄기보다 다량의 환원당과 수용성 단백질을 함유하고, collagenase 저해 활성이 높게 나타나 쇠뜨기 생식줄기와 영양줄기 모두 미용 식품 및 화장료 소 재로서의 활용 가능성이 있을 것으로 사료된다.
Abstract
Hot water extracts of fertile and sterile Equisetum arvense Linne stems were used to investigate their anti-oxidative anti-aging properties and their possible use in beauty foods or cosmetic materials. The contents of polyphenol and flavonoid compounds of fertile stem extracts were 27.06 mg/g and 45.62 mg/g, respectively. The sterile stem extract contained 191.14 mg/g of reducing sugar and 56.14 mg/g soluble protein. The nitrite scavenging abilities of fertile and sterile stem extracts at pH 1.2 and 2.0 mg/mL were 92.24% and 81.65%, respectively. At a concentration of 2.0 mg/L, the superoxide dismutase (SOD)-like activity, xanthine oxidase (XO) inhibition, tyrosinase and collagenase inhibition capacities of fertile stem extract were 24.77%, 61.48%, 28.16% and 29.68%, respectively, while those of sterile stem extract were 16.58%, 25.57%, 52.88% and 37.47%. The DPPH radical scavenging capacity at a concentration of 0.05 mg/ mL (fertile stem extract, 44.94%; sterile stem extract, 40.82%) was higher than L-ascorbic acid (36.95%), and all extracts showed greater than 90% scavenging ability even at a concentration of 0.5 mg/mL. As a result, E. arvense fertile stem extract showed the highest nitrite scavenging, SOD-like activity, xanthine oxidase inhibition, tyrosinase and collagenase inhibition, while sterile stem extract had the highest amount of reducing sugar, soluble protein and collagenase inhibition. These results suggest that hot water extracts from fertile and sterile E. arvense stems might be considered as potentially useful for the development of beauty foods and cosmetics materials.
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