마 에탄올추출물의 피부 collagen 합성 촉진 및 MMPs 활성 억제효과
Ethanol Extract of Dioscorea batatas Stimulates Procollagen Production and Reduces UVB-induced MMPs Activity in Skin
2
조회
0
다운로드
11
인용
0
참고문헌
키워드
초록
Ultraviolet (UV) B irradiation induces the production of matrix metalloproteinases (MMPs), which are responsible for the degradation or synthesis inhibition of collagenous extracellular matrix in connective tissues, causing skin photoaging. In this study, we examined the inhibitory effect of MMP-1 expression of yam extract in tumor necrosis factor-alpha (TNF-α)-stimulated human dermal fibroblast neonatal (HDFn) cell and preventive effect of UVB-induced damage in hairless mice skin. The synthesis of procollagen and the release of MMP-1 in HDFn cells were measured by EIA kit and MMP-1 assay kit, respectively. UVB radiation was applied to the backs of the mice three times a week for 8 weeks. Mice were randomly divided into three groups, and were topical application with the Dioscorea batatas (DB, 6%) or vehicle. Reduction of TNF-α-induced procollagen synthesis was increased by DB (50 ug/ml), which was higher than positive control group (TGF-β). Also, pre-treatment of HDFn cells with DB inhibited TNF-α–induced release of MMP-1. In vivo study, we found that preventive effect of DB against UV-induced epidermal thickness. DB suppressed the expression of MMP-3 and MMP-13 induced by UVB irradiation. Our results show that DB have preventive effect of UV-induced skin damage in hairless mice.
Abstract
Ultraviolet (UV) B irradiation induces the production of matrix metalloproteinases (MMPs), which are responsible for the degradation or synthesis inhibition of collagenous extracellular matrix in connective tissues, causing skin photoaging. In this study, we examined the inhibitory effect of MMP-1 expression of yam extract in tumor necrosis factor-alpha (TNF-α)-stimulated human dermal fibroblast neonatal (HDFn) cell and preventive effect of UVB-induced damage in hairless mice skin. The synthesis of procollagen and the release of MMP-1 in HDFn cells were measured by EIA kit and MMP-1 assay kit, respectively. UVB radiation was applied to the backs of the mice three times a week for 8 weeks. Mice were randomly divided into three groups, and were topical application with the Dioscorea batatas (DB, 6%) or vehicle. Reduction of TNF-α-induced procollagen synthesis was increased by DB (50 ug/ml), which was higher than positive control group (TGF-β). Also, pre-treatment of HDFn cells with DB inhibited TNF-α–induced release of MMP-1. In vivo study, we found that preventive effect of DB against UV-induced epidermal thickness. DB suppressed the expression of MMP-3 and MMP-13 induced by UVB irradiation. Our results show that DB have preventive effect of UV-induced skin damage in hairless mice.
참고문헌 (34)
- Cho, S., "Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo", J Lipid Res, 49(6), pp.1235~1245 (2008)
- Chung, J. H., "Angiogenesis in skin aging and photoaging", J Dermatol, 34(9), pp.593~600 (2007)
- Durai, P. C., "Aging in elderly : chronological versus photoaging", Indian J Dermatol, 57(5), pp.343~352 (2012)
- Fisher, G. J., "Mechanisms of photoaging and chronological skin aging", Arch Dermatol, 138(11), pp.1462~1470 (2002)
- Yang, B., "Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts", Int J Mol Med, 23(4), pp.555~560 (2009)
- Oh, J. H., "Ultraviolet B-induced matrix metalloproteinase-1 and-3 secretion are mediated via PTEN", Akt pathway in human dermal fibroblasts, 2006, pp.209(3), 775~785
- Soter, N. A, "Acute effects of ultraviolet radiation on the skin", Semin Dermatol, 9(1), pp.11~15 (1990)
- 이영래, "Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-κB pathway in human dermal fibroblasts", Experimental and Molecular Medicine, 41(8), pp.548~554 (2009), 생화학분자생물학회
- 정신교, "단마(Dioscorea aimadoimo)의 열풍건조 시 갈변 억제 방안 연구", 한국응용생명화학회지, 39(5), pp.384~388 (1996)
- 김명화, "참마 분획물이 당뇨 유발 흰주의 혈당 및 에너지대사물농도에 미치는 영향", 한국영양학회지, 31(7), pp.1093~1099 (1998)
- Kwon, C. S., "Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents", Biosci Biotechnol Biochem, 67, pp.1451~1456 (2003)
- Son, I. S., "Antioxidative and hypolipidemic effects of diosgenin, a steroidal saponin of yam(Dioscorea spp), on hing-cholesterol fed rats", Biosci Biotechnol Biochem, 71, pp.3063~3071 (2007)
- 김선여, "산약의 항당뇨 특성 연구(1) - 혈당 강하 효과 -", 한국식품영양학회지, 21(4), pp.425~429 (2008), 한국식품영양학회
- Kwon, E. G., "Effects of mucilage from yam(Dioscorea btatas DECNE)on blood glucose and lipid composition in alloxan-induced diabetic mice", Korean J Food Sci Technol, 33, pp.795~801 (2001)
- Han, Y. N., "Purification of mucilages from Dioscorea batatas and D. Japonica and their content analysis", Korean J Pharmacogn, 21, pp.274~283 (1990)
- Mosmann, T, "Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxicity assays", J Immunol Methods, 65, pp.55~63 (1983)
- Fisher, G. J., "J Clin Invest", J Clin Invest, 106(5), pp.663~670 (2000)
- Fagot, D., "Matrix metalloproteinase-1 production observed after solar-stimulated radiation exposure is assumed by dermal fibroblasts but involves a paracrine activation through epidermal keratinocyte", Photochem Photobiol, 79, pp.499~505 (2004)
- Brinckmann, J., "Collagen synthesis in(sun-)aged human skin and in fibroblasts derived from sun-exposed and sin-protected body sites", J Photochem Photobiol B, 27, pp.33~38 (1995)
- Gross, B., "Collagenolytic activity in amphibian tissue a tissue culture assay", Proc Natl Acad Sci USA, 54, pp.1197~1204 (1962)
- Steenport, M., "Matrix Metalloproteinase(MMP)-1 and MMP-3 Induce Macrophage MMP-9 : Evidence for the Role of TNF-a and Cyclooxygenase-2", J Immunol, 183(12), pp.8119~8127 (2009)
- Kim, H. H., "Augmentation of UV-induced skin wrinkling by infrared irradiation in hairless mice", Mech Ageing Dev, 126(11), pp.1170~1177 (2005)
- Kang, S. W., "Inflammatio and Extracellular Matrix Degradation Mediated by Activated ranscription Factors Nuclear Factor-kB and Activator Protein-1 in Inflammatory Acne Lesions in Vivo", Am J Pathol, 166, pp.1691~1699 (2005)
- McCord, J. M., "The utility of superoxide dismutase in studying free radical reactions. I., Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen", J Biol Chem, 25, pp.6056~6063 (1963)
- 김나미, "홍삼성분이 섬유아세포의 콜라겐 생합성과 MMP-I 활성에 미치는 영향", Journal of Ginseng Research, 31(2), pp.86~92 (2007), 고려인삼학회
- Fisher, G. J., "Pathophysiology of premature skin aging induced by ultraviolet light", N Engl J Med, 337, pp.1419~1428 (1997)
- Kim, H. H., "Photoprotective and anti-skinaging effects of eicosapentaenoic acid in human skin in vivo", J Lipid Res, 47, pp.921~930 (2006)
- Lee, K. E., "Effects of Macelignan Isolated from Myristica fragrans(Nutmeg)on Expression of Matrix Metalloproteinase-1 and Type I Procollagen in UVB-Irradiated Human Skin Fibroblasts", Biol Pharm Bull, 35(10), pp.1669~1675 (2012)
- Jean, L. B, "Aging skin", Am J Med, 8, pp.99~103 (1995)
- Laure, R. G., "UV-light-induced signal cascades and skin aging", Ageing Res Rev, 1, pp.705~720 (2002)
- Lee, Y. R., "Brazilin inhibits UVB-induced MMP-1", 3 expressions and secretions by suppressing the NF-κB pathway in human dermal fibroblasts, 2012, pp.15, 674(2-3), 80~86
- Oh, J. H., "Ultraviolet B-Induced Matrix Metalloproteinase-1 and-3 Secretions Are Mediated via PTEN", Akt Pathway in Human Dermal Fibroblasts, 2006, pp.209(3), 775~785
- Zhang, M., "IGF-1 regulation of type II collagen and MMP-13 expression in rat endplate chondrocytes via distinct signaling pathways", Osteoarthritis Cartilage, 17(1), pp.100~106 (2009)
- Mariani, T. J., "Collagenase-3 induction in rat lung fibroblasts requires the combined effects of tumor necrosis factor-alpha and 12-lipoxygenase metabolites : a model of macrophage-induced, fibroblast-driven extracellular matrix remodeling during inflammatory lung injury", Mol Biol Cell, 9(6), pp.1411~1424 (1998)