Protective Efficacy of Rhamnus davurica Methanol Extract against Photoaging in UVA-irradiated Human Dermal Fibroblasts
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In an effort to gain insight regarding the anti-photoaging efficacy of Rhamnus davurica methanol extract (RDME), the efficacies of RDME on production of reactive oxygen species (ROS), induction of apoptosis, and production of hyaluronic acid (HA) in UVA-irradiated human dermal fibroblasts (HDFs) were evaluated in the current study. The viabilities of 10, 15, and 20 mJ/cm2 UVA-irradiated HDFs were decreased by 18.3% (p < 0.001), 31.5% (p < 0.001), and 42.9% (p < 0.001), respectively. The viabilities of 10, 15, and 20 mJ/cm2 UVA-irradiated HDFs treated with 100 μg/mL RDME were increased by 6.5% (p < 0.05), 4.5%, and 21.0% (p < 0.001), respectively. Production of ROS in HDFs increased by 19.9% (p < 0.001) following UVA irradiation at 10 mJ/cm2 . However, production of ROS in 10 mJ/cm2 UVA-irradiated HDFs decreased by 15.3% (p < 0.001) following treatment with 100 μg/mL RDME. Apoptosis of HDFs increased by 449.5% (p < 0.001) following UVA irradiation at 10 mJ/cm2 . However, apoptosis of 10 mJ/cm2 UVA-irradiated HDFs decreased by 46.0% (p < 0.001) following treatment with 100 μg/mL RDME. Production of HA in HDFs decreased by 15.8% (p < 0.001) following UVA irradiation at 10 mJ/cm2 . However, production of HA in 10 mJ/cm2 UVA-irradiated HDFs increased by 16.0% (p < 0.001) by treatment with 100 μg/ mL RDME. In summary, the results reported in this study demonstrated that RDME could inhibit UVA-induced production of ROS and apoptosis of HDFs. RDME also enhanced production of HA in UVA-irradiated HDFs
Abstract
In an effort to gain insight regarding the anti-photoaging efficacy of Rhamnus davurica methanol extract (RDME), the efficacies of RDME on production of reactive oxygen species (ROS), induction of apoptosis, and production of hyaluronic acid (HA) in UVA-irradiated human dermal fibroblasts (HDFs) were evaluated in the current study. The viabilities of 10, 15, and 20 mJ/cm2 UVA-irradiated HDFs were decreased by 18.3% (p < 0.001), 31.5% (p < 0.001), and 42.9% (p < 0.001), respectively. The viabilities of 10, 15, and 20 mJ/cm2 UVA-irradiated HDFs treated with 100 μg/mL RDME were increased by 6.5% (p < 0.05), 4.5%, and 21.0% (p < 0.001), respectively. Production of ROS in HDFs increased by 19.9% (p < 0.001) following UVA irradiation at 10 mJ/cm2 . However, production of ROS in 10 mJ/cm2 UVA-irradiated HDFs decreased by 15.3% (p < 0.001) following treatment with 100 μg/mL RDME. Apoptosis of HDFs increased by 449.5% (p < 0.001) following UVA irradiation at 10 mJ/cm2 . However, apoptosis of 10 mJ/cm2 UVA-irradiated HDFs decreased by 46.0% (p < 0.001) following treatment with 100 μg/mL RDME. Production of HA in HDFs decreased by 15.8% (p < 0.001) following UVA irradiation at 10 mJ/cm2 . However, production of HA in 10 mJ/cm2 UVA-irradiated HDFs increased by 16.0% (p < 0.001) by treatment with 100 μg/ mL RDME. In summary, the results reported in this study demonstrated that RDME could inhibit UVA-induced production of ROS and apoptosis of HDFs. RDME also enhanced production of HA in UVA-irradiated HDFs
참고문헌 (20)
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