대추나무 새순 추출물의 보습 및 항염증 효능
Antioxidant, Moisturizing Activities and Anti-inflammation of Ziziphus jujuba Miller Bud Extract
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In this study, Ziziphus jujuba Miller bud were extracted with hot water (ZBD) and 70% ethanol (ZBE) to examine the potential for antioxidant, anti-inflammatory, and moisturizing effects. The results of measuring the total polyphenol content showed that the contents of ZBD and ZBE were 525.39 ±0.94 mg TAE/100 g and 581.63 ±0.94 mg TAE/100 g, respectively. Regarding electronic donating ability, at a concentration of 1,000 μg/mL, ZBD and ZBE were 76.06% and 80.92%, respectively, and the results of 2,2’-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical scavenging showed that both extracts exerted excellent antioxidant activity of greater than 99% at a concentration of 500 μg/mL. Cell experiments were then performed according to the results of RAW 264.7 and HaCaT cytotoxicity measurements. At a concentration of 500 μg/mL, the results of measuring nitric oxide inhibitory activity showed that both extracts exerted an inhibitory activity of greater than 11% compared to the lipopolysaccharide (LPS) treatment group alone, and the amount of hyaluronic acid production by ZBE was greater than 126% compared to the groups treated with P. acnes alone. By confirming the protein expression of iNOS and COX-2 compared to the LPS treatment group alone, at a concentration of 500 μg/mL, ZBD inhibited 24.21% and 29.78% and the inhibitory efficacy of ZBE was 68.83% and 47.71%, respectively. Therefore, ZBD and ZBE can be regarded as being effective in exerting anti- inflammatory and moisturizing effects and can be used as a material for natural cosmetic.
Abstract
In this study, Ziziphus jujuba Miller bud were extracted with hot water (ZBD) and 70% ethanol (ZBE) to examine the potential for antioxidant, anti-inflammatory, and moisturizing effects. The results of measuring the total polyphenol content showed that the contents of ZBD and ZBE were 525.39 ±0.94 mg TAE/100 g and 581.63 ±0.94 mg TAE/100 g, respectively. Regarding electronic donating ability, at a concentration of 1,000 μg/mL, ZBD and ZBE were 76.06% and 80.92%, respectively, and the results of 2,2’-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical scavenging showed that both extracts exerted excellent antioxidant activity of greater than 99% at a concentration of 500 μg/mL. Cell experiments were then performed according to the results of RAW 264.7 and HaCaT cytotoxicity measurements. At a concentration of 500 μg/mL, the results of measuring nitric oxide inhibitory activity showed that both extracts exerted an inhibitory activity of greater than 11% compared to the lipopolysaccharide (LPS) treatment group alone, and the amount of hyaluronic acid production by ZBE was greater than 126% compared to the groups treated with P. acnes alone. By confirming the protein expression of iNOS and COX-2 compared to the LPS treatment group alone, at a concentration of 500 μg/mL, ZBD inhibited 24.21% and 29.78% and the inhibitory efficacy of ZBE was 68.83% and 47.71%, respectively. Therefore, ZBD and ZBE can be regarded as being effective in exerting anti- inflammatory and moisturizing effects and can be used as a material for natural cosmetic.
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