동의보감의 한방조성물에서 추출한 에센셜오일의 항바이러스효과
Antiviral Effects of Essential Oil Extracted from Oriental Herb Composition in Donguibogam
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Essential oils derived from an oriental medicine composition from Donguibogam were applied for study of the development of formulations with an anti-viral effect in humans. Herbal materials, including Coptis japonica, Scutellaria baicalensis, Phellodendron amurense, Gardenia Jasminoides, Ledebouriella seseloides, Astragalus membranaceus, Atractylodes japonica, Platycodon grandiflorus, Camellia Sinensis, Hovenia dulcis, Ulmus macrocarpa, and Pinus densiflora; fermentation materials, including Aspergillus oryzae and Lactobacillus plantarum, and formulation materials, such as MCT oil were used. A distillation method was used for extraction of essential oils from the herbal materials. The antiviral effect was tested using the prepared oil-phase formulation, and a reducing effect of 82.22–92.06% was confirmed at 1% of the standard content of the essential oil. The results of GC-MS analysis showed that the herbal composition contained pinene, limonene, terpinolene, and p-cymene, which are indicator components of pine trees. The antioxidant effect of P-cymene was positive for stability. The difference in retention time between the herbal composition and the fermented herbal composition was 32.60 min. Below that, the peak for the herbal composition was slightly higher, and above that, the peak for the fermented herbal composition was higher. In addition, the fermented herbal medicine composition included vetiverol, estragole, and alloocimene. Because essential oils extracted from materials used in oriental medicine have the same properties as organic solvents, skin corrosion tests were performed to confirm that the level of corrosive properties was low. Cytotoxicity testing was also performed, and the results showed that essential oil formulations containing less than 10% had no cytotoxicity. Herbal materials including essential oils will be helpful in development of antiviral products such as medicinal cosmetics, medical devices, etc
Abstract
Essential oils derived from an oriental medicine composition from Donguibogam were applied for study of the development of formulations with an anti-viral effect in humans. Herbal materials, including Coptis japonica, Scutellaria baicalensis, Phellodendron amurense, Gardenia Jasminoides, Ledebouriella seseloides, Astragalus membranaceus, Atractylodes japonica, Platycodon grandiflorus, Camellia Sinensis, Hovenia dulcis, Ulmus macrocarpa, and Pinus densiflora; fermentation materials, including Aspergillus oryzae and Lactobacillus plantarum, and formulation materials, such as MCT oil were used. A distillation method was used for extraction of essential oils from the herbal materials. The antiviral effect was tested using the prepared oil-phase formulation, and a reducing effect of 82.22–92.06% was confirmed at 1% of the standard content of the essential oil. The results of GC-MS analysis showed that the herbal composition contained pinene, limonene, terpinolene, and p-cymene, which are indicator components of pine trees. The antioxidant effect of P-cymene was positive for stability. The difference in retention time between the herbal composition and the fermented herbal composition was 32.60 min. Below that, the peak for the herbal composition was slightly higher, and above that, the peak for the fermented herbal composition was higher. In addition, the fermented herbal medicine composition included vetiverol, estragole, and alloocimene. Because essential oils extracted from materials used in oriental medicine have the same properties as organic solvents, skin corrosion tests were performed to confirm that the level of corrosive properties was low. Cytotoxicity testing was also performed, and the results showed that essential oil formulations containing less than 10% had no cytotoxicity. Herbal materials including essential oils will be helpful in development of antiviral products such as medicinal cosmetics, medical devices, etc
참고문헌 (21)
- Alsharif A, "Selective dehydroisomerization of cyclic monoterpenes to p-cymene over silica-supported CdO", Appl Catal, 325, pp.122362~ (2023)[DOI]
- Cazedey ECL, "Corrositex®, BCOP and HET-CAM as alternative methods to animal experimentation", Braz J Pharm Sci, 45, pp.759~766 (2009)[DOI]
- Chan RY, "The effects of two Chinese herbal medicinal formulae vs. placebo controls for treatment of allergic rhinitis : a randomised controlled trial", Trials, 15, pp.261~ (2014)
- 김대덕, "국산 소나무껍질추출물(파인엑솔R)을 함유한 제제의 피부흡수 평가", Journal of Pharmaceutical Investigation, 37(6), pp.359~364 (2007), 한국약제학회
- 최수한, "소아청소년 코로나바이러스감염증-19의 치료: 치료 약제를 중심으로", Pediatric Infection and Vaccine, 29(1), pp.1~15 (2022), 대한소아감염학회[DOI]
- Dijkman R, "The dominance of human coronavirus OC43 and NL63 infections in infants", J Clin Virol, 53, pp.135~139 (2012)
- Ghasemi M, "The MTT assay : utility, limitations, pitfalls, and interpretation in bulk and single-cell analysis", Int J Mol Sci, 22, pp.12827~ (2021)[DOI]
- 홍철희, "황련해독탕 약침치료를 병행한 지루성피부염 치험 2례", 한방안이비인후피부과학회지, 25(2), pp.68~75 (2012), 대한한방안이비인후피부과학회[DOI]
- Jianming C, "Research in the effect of Yupingfeng powder on the susceptibility to influenza in children with recurrent respiratory tract infection of different TCM types", Chin Pediatr Integr Tradit West Med, 10, pp.16~20 (2018)
- 기호필, "黃連解毒湯의 아토피 피부염 개선 效果", 대한한의학 방제학회지, 21(1), pp.80~90 (2013), 대한한의학방제학회
- 김보경, "김치 및 김치 유래 유산균의 건강 기능성에 대한 연구 동향 조사", Journal of Nutrition and Health, 51(1), pp.1~13 (2018), 한국영양학회[DOI]
- 김형률, "東醫寶鑑 中 黃連解毒湯의 活用에 대한 硏究", 한방안이비인후피부과학회지, 22(1), pp.195~218 (2009), 대한한방안이비인후피부과학회
- 김태우, "동아시아 의서와 의료실천: 동의보감학파들을 통해 읽는 텍스트와 실천 그리고 동아시아의학 지식", 의사학, 28(2), pp.591~619 (2019), 대한의사학회
- Li C, "Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect : Impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway", Pharmacol, 152, pp.104603~ (2020)[DOI]
- 이병진, "도라지 뿌리의 건조방법에 따른 무기 성분 및 사포닌 함량의 변화", 한국식품과학회지, 46(5), pp.636~640 (2014), 한국식품과학회[DOI]
- Oliveira TM, "Evaluation of p-cymene, a natural antioxidant", Pharm Biol, 53, pp.423~428 (2015)
- Pan C, "Role of gut microbiota in the pharmacological effects of natural products", Evid Based Complement Alternat Med, pp.7~ (2019)[DOI]
- Smith RL, "Safety assessment of allylalkoxybenzene derivatives used as flavouring substances : methyl eugenol and estragole", Food Chem Toxicol, 40, pp.851~870 (2002)
- Yang CW, "Repurposing old drugs as antiviral agents for coronaviruses", Biomed J, 43, pp.368~374 (2020)
- 양현주, "황련해독탕 및 발효황련해독탕의 항염증 효과", 한방안이비인후피부과학회지, 24(2), pp.1~15 (2011), 대한한방안이비인후피부과학회
- Zeng WC, "Chemical composition, antioxidant, and antimicrobial activities of essential oil from pine needle(Cedrus deodara)", J Food Sci, 77, pp.824~829 (2012)