Anti-Porcine Epidemic Diarrhea Virus (PEDV) Activity and Antimicrobial Activities of Artemisia dubia Essential Oil
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The chemical composition, anti-porcine epidemic diarrhea virus (PEDV) activity and antimicrobial activity of Artemisia dubia essential oil were evaluated in this study. Fifty eight compounds from A. dubia essential oil were identified through analysis by gas chromatography-mass spectrometry (GC-MS). The major constituents of the oil were camphor (17.18 %), germacrene-D (15.70%), trans (β-) racaryophyllene (6.79%), ene thujones (6.57%), 1, 8-cineole (5.94%) and camphene (5.08%). The essential oil was evaluated for antiviral activity against PEDV in Vero cells using a cytopathic effect (CPE) reduction method. The oils actively inhibited PEDV replication with a 50% inhibitory concentration (IC50) of 43.7 μ3/mL. The 50% cytotoxicity concentration (CC50) of the oils was over 100 μ/mL and the derived therapeutic index was >2.3. Similar analysis of the ribavirin revealed that they have a relatively weaker efficacy when compared to the oils. The antimicrobial activity of the essential oil against 5 microorganisms was evaluated by the disc diffusion method. The essential oil exhibited antimicrobial activity against 5 tested microorganisms with a clear zone of 8-22 mm. Among the tested microorganisms, Streptococcus pyogenes was the most sensitive and Candida albicans the least. Therefore, in can be concluded that essential oils of A. dubia may have interesting applications for microbial control or the control of PEDV-derived diseases.
Abstract
The chemical composition, anti-porcine epidemic diarrhea virus (PEDV) activity and antimicrobial activity of Artemisia dubia essential oil were evaluated in this study. Fifty eight compounds from A. dubia essential oil were identified through analysis by gas chromatography-mass spectrometry (GC-MS). The major constituents of the oil were camphor (17.18 %), germacrene-D (15.70%), trans (β-) racaryophyllene (6.79%), ene thujones (6.57%), 1, 8-cineole (5.94%) and camphene (5.08%). The essential oil was evaluated for antiviral activity against PEDV in Vero cells using a cytopathic effect (CPE) reduction method. The oils actively inhibited PEDV replication with a 50% inhibitory concentration (IC50) of 43.7 μ3/mL. The 50% cytotoxicity concentration (CC50) of the oils was over 100 μ/mL and the derived therapeutic index was >2.3. Similar analysis of the ribavirin revealed that they have a relatively weaker efficacy when compared to the oils. The antimicrobial activity of the essential oil against 5 microorganisms was evaluated by the disc diffusion method. The essential oil exhibited antimicrobial activity against 5 tested microorganisms with a clear zone of 8-22 mm. Among the tested microorganisms, Streptococcus pyogenes was the most sensitive and Candida albicans the least. Therefore, in can be concluded that essential oils of A. dubia may have interesting applications for microbial control or the control of PEDV-derived diseases.
참고문헌 (26)
- quadrupole mass spectroscopy, Allured Publishing Corporation, "Identification of essential oils components by gas chromatography", pp.2001, Adams, R. P
- Blagojevic, P, "Chemical composition of the essential oils of serbian wild-growing Artemisia absinthium and Artemisia vulgaris", J. Agric. Food Chem, 54, pp.4780~4789 (2006)
- Burt, S. A., "Essential oils: their antibacterial properties and potential applications in foods−a review", Int. J. Food Microbiol, 94, pp.223~253 (2004)
- Chagonda, L. S, "The essential oil of cultivated Artemisia afra (Jacq.) from Zimbabwe", Flav. Fragr. J, 14, pp.140~142 (1999)
- Chericoni, S, "GC-MS analyses of the essential oil from the aerial parts of Artemisia verlotiorum: variability during the year", Biochem. Syst. Ecol, 32, pp.423~429 (2004)
- Choi, H. J, "Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus", Antiviral Res, 81, pp.77~81 (2008)
- Conner, D. E., "Naturally occurring compounds, IN Antimicrobials in foods", pp.441~468 (1993), Marcel Dekker
- Deans, S. G., "Volatile Oil Crops: Their Biology", pp.113~ (1993), Biochemistry and Production
- Denyer, S. P, "Biocide-induced damage to the bacterial cytoplasmic membrane, In Mechanisms of Action of Chemical Biocides, The Society for Applied Bacteriology, Technical Series", pp.171~188 (1991), Oxford Blackwell Scientific Publication
- Dorman, H. J. D, "Antimicrobial agents from plants: antibacterial activity of plant volatile oils", J. Appl. Microbiol, 88, pp.308~316 (2000)
- Juteau, F, "Antibacterial and antioxidant activities of Artemisia annua essential oil", Fitoterapia, 73, pp.532~535 (2002)
- Kalemba, D, "Antimicrobial properties of the essential oil of Artemisia asiatica Nakai", Phytother. Res, 16, pp.288~291 (2002)
- Khaled, F, "Antioxidant activity and volatile components of Egyptian Artemisia judaica L", Food Chem, 79, pp.331~336 (2002)
- Khan, M. T. H, "Extracts and molecules from medicinal plants against herpes simplex viruses", Antiviral Res, 67, pp.107~119 (2005)
- Khazraei-Alizadeh, K, "Composition of the volatile oil of Artemisia diffusa Krasch ex Poljak growing wild in Iran", J. Essent. Oil Res, 13, pp.185~186 (2001)
- Kordali, S, "Screening of chemical composition and antifungal and antioxidant activities of the essential oils from three Turkish Artemisia species", J. Agric. Food Chem, 53, pp.1408~1416 (2005)
- Kordali, S, "Determination of the Chemical Composition and Antioxidant Activity of the Essential Oil of Artemisia dracunculus and of the Antifungal and Antibacterial Activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum, and Artemisia spicigera Essential Oils", J. Agric. Food Chem, 53, pp.9452~9458 (2005)
- Loizzo, M. R, "Phytochemical analysis and in vitro evaluation of the biological activity against herpes simplex virus type 1 (HSV-1) of Cedrus libani A", Rich. Phytomed, 15, pp.79~83 (2008)
- Murray, P. R, "Manual of clinical microbiology, 6th Ed" (1995), ASM
- Pensaert, M. B, "Porcine epidemic diarrhea, In Diseases of Swine", pp.179~185 (1999), Iowa State University Press
- Pino, J. A, "Chemical composition of the essential oil of Artemisia dracunculus L. from Cuba", J. Essent. Oil res, 8, pp.563~564 (1996)
- Schnitzler, P, "Antiviral activity of Australian tea tree oil and eucalyptus oil against herpes simplex virus in cell culture", Pharmazie, 56, pp.343~347 (2001)
- Schuhmacher, A, "Virucidal effect of peppermint oil on the enveloped viruses herpes simplex virus type 1 and type 2 in vitro", Phytomed, 10, pp.504~510 (2003)
- Sikkema, J, "Interactions of cyclic hydrocarbons with biological membranes", J. Biol. Chem, 269, pp.8022~8028 (1994)
- Singh, A. P, "Flowering plants of Nepal (Phanerogams), Bull. Dept. of Plant resources, In Ministry of Forests and Soil conservation, Department of Plant resources, National Herbarium and Plant Laboratories", pp.3999~ (2001), Godavary
- Song, D. S, "Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain", Research in Veterinary Science, 82(1), pp.134~140, W. B. Saunders Co., Ltd.