Hair Loss Improvement Efficacies of Ethanolic Extracts of Ribes fasciculatum and Callicarpa japonica in UVB-irradiated Human Dermal Papilla Cells
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The purpose of this study was to examine the hair loss inhibitory efficacies of ethanolic extracts of Ribes fasciculatum (RFEE) and Callicarpa japonica (CJEE) in 20 mJ/cm2 UVB-irradiated human dermal papilla cells (hDPCs) by measuring transforming growth factor (TGF)-β, interleukin (IL)-1α mRNA, and reactive oxygen species (ROS). In the results of MTT assay, the cell viabllity of hDPCs irradiated with 20 mJ/cm2 UVB was 91.7%, and the results for those for 25 μg/mL treatments with RFEE and CJEE as well as 1 μM MXD after UVB irradiation were over 90%. Thus, the maximum permissible level of both RFEE and CJEE to hDPCs was over 25 μg/mL. In the results of the quantitative RT-PCR assay, treatment with 25 μg/mL RFEE and CJEE, and 1 μM MXD resulted in decreased expression of TGF-β mRNA by 27.3% (p < 0.001), 52.3% (p < 0.001), and 33.9% (p < 0.001), respectively, decreased expression of IL-1α mRNA by 48.7% (p < 0.001), 59.8% (p < 0.001), and 29.1% (p < 0.001), respectively, and decreased production of ROS by 9.5% (p < 0.01), 20.2% (p < 0.001), and 46.8% (p < 0.001), respectively, compared to the control.
Abstract
The purpose of this study was to examine the hair loss inhibitory efficacies of ethanolic extracts of Ribes fasciculatum (RFEE) and Callicarpa japonica (CJEE) in 20 mJ/cm2 UVB-irradiated human dermal papilla cells (hDPCs) by measuring transforming growth factor (TGF)-β, interleukin (IL)-1α mRNA, and reactive oxygen species (ROS). In the results of MTT assay, the cell viabllity of hDPCs irradiated with 20 mJ/cm2 UVB was 91.7%, and the results for those for 25 μg/mL treatments with RFEE and CJEE as well as 1 μM MXD after UVB irradiation were over 90%. Thus, the maximum permissible level of both RFEE and CJEE to hDPCs was over 25 μg/mL. In the results of the quantitative RT-PCR assay, treatment with 25 μg/mL RFEE and CJEE, and 1 μM MXD resulted in decreased expression of TGF-β mRNA by 27.3% (p < 0.001), 52.3% (p < 0.001), and 33.9% (p < 0.001), respectively, decreased expression of IL-1α mRNA by 48.7% (p < 0.001), 59.8% (p < 0.001), and 29.1% (p < 0.001), respectively, and decreased production of ROS by 9.5% (p < 0.01), 20.2% (p < 0.001), and 46.8% (p < 0.001), respectively, compared to the control.
참고문헌 (55)
- Achenbach H, "Alkaloids and other compounds from seeds of Tabernaemontana cymosa", Phytochem, 45, pp.325~335 (1997)
- Achenbach H, "New lignan glucosides from Stemmadenia minima", Planta Med, 58, pp.270~272 (1992)
- Amory JK, "The effect of 5α-reductase inhibition with dutasteride and finasteride on semen parameters and serum hormones in healthy men", J Clin Endocrinol Metab, 92, pp.1659~1665 (2007)
- Anita KG, "Prashant S : Atypical post‑finasteride syndrome : a pharmacological riddle", Indian J Pharmacol, 48, pp.316~ (2016)
- Arck PC, "Towards a "free radical theory of graying": melanocyte apoptosis in the aging human hair follicle is an indicator of oxidative stress induced tissue damage", FASEB J, 20, pp.1567~1569 (2006)
- Benndorf RA, "Isoprostanes inhibit vascular endothelial growth factor-induced endothelial cell migration, tube formation, and cardiac vessel sprouting in vitro, as well as angiogenesis in vivo via activation of the thromboxane A2 receptor: a potential link between oxidative stress and impaired angiogenesis", Circ Res, 103, pp.1037~1046 (2008)
- Boivin WA, "Influence of interleukin-1α on androgen receptor expression and cytokine secretion by cultured human dermal papilla cells", Exp Dermatol, 15, pp.784~793 (2006)
- Cairns JI, "Murine rIL-1α stimulates proliferation and collagenase secretion by cultured hair follicles from neonatal mice(abstract)", J Invest Dermatol, 92, pp.410~ (1989)
- Danilenko DM, "Keratinocyte growth factor is an important endogenous mediator of hair follicle growth, development, and differentiation", Am J Pathol, 145, pp.147~154 (1995)
- Dat NT, "New inhibitor against nuclear factor of activated T cells transcription from Ribes fasciculatum var. chinense", Chem Pharm Bull (Tokyo), 53, pp.114~117 (2005)
- Foitzik K, "Control of murine hair follicle regression(catagen)by TGF-β1 in vivo", FASEB J, 14, pp.752~760 (2000)
- Friedman ES, "Allergic contact dermatitis to topical minoxidil solution: etiology and treatment", J Am Acad Dermatol, 46, pp.309~312 (2002)
- Groves RW, "Analysis of epidermal IL-1 family members in vivo using transgenic mouse models", J Investig Dermatol, 102, pp.556~ (1994)
- Hamada K, "Pro-inflammatory cytokine interleukin-1α is downregulated during anagen phase of hair cycle in vivo", J Dermatol Sci, 33, pp.195~198 (2003)
- Herman A, "Mechanism of action of herbs and their active constituents used in hair loss treatment", Fitoterapia, 114, pp.18~25 (2016)
- Hibino T, "Role of TGF-β2 in the human hair cycle", J Dermatol Sci, 35, pp.9~18 (2004)
- Hmid AB, "FOXP3 transcription is enhanced in lesional and perilesional skin of patients with focal Alopecia areata", Int J Dermatol, 54, pp.e319~321 (2015)
- Hoffmann R, "Cytokines and growth factors influence hair growth in vitro. Possible implications for the pathogenesis and treatment of alopecia areata", Arch Dermatol Res, 288, pp.153~156 (1996)
- Hoffmann R, "Elements of the interleukin-1 signaling system show hair cycle-dependent gene expression in murine skin", Eur J Dermatol, 8, pp.475~477 (1998)
- Hoffmann R, "Does interleukin-1 induce hair loss?", Dermatology, 191, pp.273~275 (1995)
- Houschyar KS, "Molecular mechanisms of hair growth and regeneration: current understanding and novel paradigms", Dermatology, 236, pp.271~280 (2020)
- Hu R, "Combined treatment with oral finasteride and topical minoxidil in male androgenetic alopecia: a randomized and comparative study in Chinese patients", Dermatol Ther, 28, pp.303~308 (2015)
- Huang WY, "Stress-induced premature senescence of dermal papilla cells compromises hair follicle epithelial-mesenchymal interaction", J Dermatol Sci, 86, pp.114~122 (2017)
- Inui S, "Androgen-inducible TGF- β1 from balding dermal papilla cells inhibits epithelial cell growth: a clue to understand paradoxical effects of androgen on human hair growth", FASEB J, 16, pp.1967~1969 (2002)
- Inui S, "Identification of androgen-inducible TGF-β1 derived from dermal papilla cells as a key mediator in androgenetic alopecia", J Investig Dermatol Symp Proc, 8, pp.69~71 (2003)
- Irwig MS, "Persistent sexual side effects of finasteride for male pattern hair loss", J Sex Med, 8, pp.1747~1753 (2011)
- Ito T, "Interferon-γ is a potent inducer of catagen-like changes in cultured human anagen hair follicles", British J Dermatol, 152, pp.623~631 (2005)
- Jadkauskaite L, "Oxidative stress management in the hair follicle: could targeting NRF2 counter age-related hair disorders and beyond?", Bioessays, 39, pp.1700029~ (2017)
- Kerstin Foitzik GL, "Control of murine hair follicle regression(catagen)by TGF-β1 in vivo", FASEB J, 14, pp.752~760 (2000)
- Kim YM, "Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through Wnt", catenin pathway, 2017, pp.61, 1412792~
- Kwack MH, "Dihydrotestosterone-inducible IL-6 inhibits elongation of human hair shafts by suppressing matrix cell proliferation and promotes regression of hair follicles in mice", J Invest Dermatol, 132, pp.43~49 (2012)
- Lee SW, "A systematic review of topical finasteride in the treatment of androgenetic alopecia in men and women", J Drugs Dermatol, 17, pp.457~463 (2018)
- Mahe′ YF, "Pro-inflammatory cytokine cascade in human plucked hair", Skin Pharmacol, 9, pp.366~375 (1996)
- Moloney JN, "ROS signalling in the biology of cancer", Semin Cell Dev Biol, 80, pp.50~64 (2018)
- Naito A, "Lipid peroxides induce early onset of catagen phase in murine hair cycles", Int J Mol Med, 22, pp.725~729 (2008)
- Ohashi K, "Indonesian medical plants. XII. Four isomeric lignan-glucosides from the bark of Aegle marmelos(Rutaceae)", Chem Pharm Bull, 42, pp.1924~1926 (1994)
- Paus R, "The biology of hair follicles", N Engl J Med, 341, pp.491~497 (1999)
- Rho SS, "The hair growth promoting effect of Asiasari radix extract and its molecular regulation", J Dermatol Sci, 38, pp.89~97 (2005)
- Rishikaysh P, "Signaling involved in hair follicle morphogenesis and development", Int J Mol Sci, 15, pp.1647~1670 (2014)
- Sakushima A, "Dihydrobenzofuran lignans from Boreava orientalis", Phytochem, 43, pp.1349~1354 (1996)
- Samplaski MK, "Finasteride use in the male infertility population: effects on semen and hormone parameters", Fertil Steril, 100, pp.1542~1546 (2013)
- Talavera-Adame D, "Newman N : Conventional and novel stem cell-based therapies for androgenic alopecia", Stem Cells Cloning, 10, pp.11~19 (2017)
- Trüeb RM, "Oxidative stress in ageing of hair", Int J Trichology, 1, pp.6~14 (2009)
- Trüeb RM, "The impact of oxidative stress on hair", Int J Cosmet Sci, 37, pp.25~30 (2015)
- Upton JH, "Oxidative stress-associated senescence in dermal papilla cells of men with androgenetic alopecia", J Invest Dermatol, 135, pp.1244~1252 (2015)
- Włodarczyk M, "Obesity, DNA damage, and development of obesity-related diseases", Int J Mol Sci, 20, pp.1146~ (2019)
- Wu H, "Promoting angiogenesis in oxidative diabetic wound microenvironment using a nanozyme-reinforced self-protecting hydrogel", ACS Cent Sci, 5, pp.477~485 (2019)
- Yagi K, "Lipid peroxides and human diseases", Chem Phys Lipids, 45, pp.337~351 (1987)
- Yamasaki T, "Two new lignan glucosides from the fruit of Vitex cannabifolia", J Nat Med, 6, pp.47~51 (2008)
- Yamasaki T, "A new phenylethanoid glycoside from the fruits of Callicarpa japonica Thunb. var. luxurians Rehd", J Nat Med, 61, pp.318~322 (2007)
- Yano K, "Control of hair growth and follicle size by VEGF-mediated angiogenesis", J Clin Invest, 107, pp.409~417 (2001)
- Yoo DY, "Inhibitive ability of extracts from Ribes fasciculatum and Calicarpa japonica on oxidative stress in a cell-free system and their cellular viability in human dermal papilla cells", J Invest Cosmetol, 19, pp.81~86 (2023)[DOI]
- Yoo DY, "Polyphenol and flavonoid contents and electron-donating activities of ethanolic and methanolic extracts from Ribes fasciculatum and Calicarpa japonica in a cell-free system", J Invest Cosmetol, 19, pp.1~8 (2023)[DOI]
- Yuasa K, "Lignan and neolignan glycosides from stems of Alangium premnifolium", Phytochem, 45, pp.611~615 (1997)
- Zhao J, "Foxp1 regulates the proliferation of hair follicle stem cells in response to oxidative stress during hair cycling", PLoS One, 10, pp.e0131674~ (2015)
