저장 조건이 녹차 음료의 품질에 미치는 영향
Effect of Storage Conditions on the Quality of Green Tea Beverage
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초록
녹차 잎 1.5 g에 증류수 100 mL을 가하여 75℃에서 5분 동안 추출하여 녹차 음료를 제조하였다. 저장 조건을 달리하여 A 녹차 음료 (빛을 인위적으로 차단하지 않음, 자연 상태의 대기 조건, 비타민 C 무첨가), B 녹차 음료 (빛을 알루미늄 호일로 감싸서 차단함, 질소를 불어넣어 자연 대기를 질소로 치환하였음, 비타민 C 30 mg/100 mL 첨가), C 녹차 음료 (빛을 알루미늄 호일로 감싸서 차단함, 질소를 불어넣어 자연 대기를 질소로 치환하였음, 비타민 C 무첨가)로 구분하여 4℃에서 28일간 저장한 후, 유용 성분을 분석하였다. 총페놀함량의 경우 저장 초기에 비하여 저장 28일 후 A 녹차 음료는 71.50%, B 녹차 음료는 73.88%, C 녹차 음료는 75.07%로 감소하였다. DPPH 라디컬 소거능은 저장 초기에 비하여 A 녹차 음료는 87.87%, B 녹차 음료는 92.93%, C 녹차 음료는 88.39%이었다. 녹차의 유용 카테킨인 epigallocatechin gallate (EGCG)는 28일간 저장 후에 A 녹차 음료는 130.61%, B 녹차 음료는 136.47%, C 녹차 음료는 4.34%이었다. 이상의 결과는 녹차의 저장 조건이 녹차 음료의 성분에 영향을 미치며, 빛, 질소 치환, 비타민 C가 매우 중요함을 시사하였다.
Abstract
Green tea was prepared by soaking 1.5 g of green tea leaves into 100 mL of distilled water at 75℃ for 5 min. The green tea was stored at three different conditions - (A) green tea was stored at not-artificially excluding natural light condition, at natural air condition, and without addition of vitamin C; (B) green tea was stored at artificially excluding natural light condition by wrapping a vial with aluminium foil, at nitrogen filling up condition, and with addition of 30 mg/100 mL of vitamin C; and (C) green tea was stored at artificially excluding natural light condition by wrapping a vial with aluminium foil, at nitrogen filling up condition, and without addition of vitamin C. After 28 days of storage at 4℃, the chemical quality of the green tea was evaluated. Total phenolic contents of (A), (B), and (C) green tea decreased to 71.50, 73.88 and 75.07%, respectively, after storing for 28 days compared to those of beginning state. DPPH radical scavenging activities of (A), (B), and (C) green tea were 87.87, 92.93 and 88.39%, respectively. Epigallocatechin gallate (EGCG), the main active compounds of green tea, contents of (A), (B), and (C) green tea were 130.61, 136.47 and 4.34%, respectively. The results indicated that light, air condition, and vitamin C were significantly important to the chemical quality of green tea during storage.
참고문헌 (26)
- Balasubramanian, S, "Green tea polyphenol stimulates a Ras, MEKK1, MEK3, and p38 cascade to increase activator protein 1 factor-dependent involucrin gene expression in normal keratinocytes", J. Biol. Chem, 277, pp.1828~1836 (2002)
- Blois, M. S, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Choi, H. J., "Changes in chemical compositions of green tea(Camellia sinensis L.)under the different extraction conditions.Korean J.Life Sci.10:202-209", Korean J. Life Sci, 10, pp.202~209 (2000)
- Conney, A. H., "Inhibition effect green tea on tumor growth on mouse skin. In phenolic compounds in food and their effects on health II", ACS Symposium Series, 507, pp.284~291 (1992), American Chemical Society
- Crespy, V, "A review of the health effects of green tea catechins in in vivo animal models", J. Nutr, 134, pp.3431S~3440S (1994)
- Gutfinger, T, "Polyphenols in olive oils", J. Am. Oil Chem. Soc., 58, pp.966~968 (1981)
- Kaoahachi, O, "New compendium of tea work(storage and packing method of green tea)", pp.153~203 (1988), Shan Hyob Printing Co.
- Katiyar, S. K., "Inhibition of UVB-induced oxidative stress-mediated phosphorylation of mitogen-activated protein kinase signaling pathways in cultured human epidermal keratinocytes by green tea polyphenol (-) epigallocatechin-3-gallate", Toxicol. Appl. Pharmacol, 176, pp.110~117 (2001)
- 고정삼, "저장조건에 따른 녹차음료의 기능성성분 변화 및 안정성", 한국식품저장유통학회지, 13(4), pp.421~426 (2006), 한국식품저장유통학회
- Koh, S. H., "Epigallocatechin gallate prevents oxidative-stress-induced death of mutant Cu", Zn-superoxide dismutase(G93A) motoneuron cells by alteration of cell survival and death signals, 2004, pp.202, 213~225
- Lee, S. C., "Effect of far-infrared irradiation on catechins and nitrite scavenging activity of green tea", J. Agric. Food Chem, pp.399~403 (2006)
- Liang, Y. C, "Suppression of extracellular signals and cell proliferation through EGF receptor binding by (-)-epigallocatechin gallate in human A431 epidermoid carcinoma cells", J. Cell. Biochem, 67, pp.55~65 (1998)
- Matsumura,K, "Chemistry of tea", pp.36~38 (2002), Asakura Press
- Nagle, D. G., "Epigallocatechin-3-gallate (EGCG): chemical and biomedical perspectives", Phytochemistry, 67, pp.1849~1855 (2006)
- 박장현, "가루녹차의 저온저장(-5℃) 중 품질 변화", 한국식품저장유통학회지, 13(6), pp.681~685 (2006), 한국식품저장유통학회
- Peng, G., "Anti-proliferative effects of green tea polyphenol EGCG on Ha-Ras-induced transformation of intestinal cells", Cancer Lett, 238, pp.260~270 (2006)
- Price, M. L., "A critical evaluation of the vanillin reaction as assay for tannin in sorghum grain", J. Agric. Food Chem, 26, pp.1214~1218 (1978)
- Sachinidis, A., "Green tea compounds inhibit tyrosine phosphorylation of PDGF β-receptor and transformation of A172 human glioblastoma", FEBS Lett, 471, pp.51~55 (2000)
- SAS Institute, "SAS", STAT User’s Guide, 1995
- Sikic, B. I., "The effects of ascorbic of ascorbic acid deficiency and repletion on pulmonary, renal and hepatic drug metabolism in the guinea pig", Arch. Biochem. Biophys., 179, pp.663~671 (1977)
- Wang, L. F., "Effect of heat processing and storage on flavanols and sensory qualities of green tea beverage", J. Agirc. Food Chem, 48, pp.4227~4232 (2000)
- Wang, H., "HPLC determination of catechins in tea leaves and tea extracts using relative response factors", Food Chem, 81, pp.307~312 (2003)
- Williams, R. J., "Flavonoids: anti- oxidants or signalling molecules", Free Radic. Biol. Med, 36, pp.838~849 (2004)
- Wolfram, S, "Effects of green tea and EGCG on cardiovascular and metabolic health", J. Am. Coll. Nutr., 26, pp.373S~388S (2007)
- Zhang, A., "Inhibitory effects of jasmine green tea epicatechin isomers on free radical-induced lysis of blood cells", Life Sci., 61, pp.384~394 (1997)
- 정동효, "차의 화학성분과 기능", 7~25 (2005)