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호박 흰가루병 발병에 의한 산화적 스트레스 유기와 항산화효소의 활성화

nduction of Oxidative Stress and Activation of Antioxidant Enzymes by Infection of Powdery Mildew in Cucurbita Plants

김희은|강남준|이희주|허윤찬|박동금|황미란
농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)|2013|농업생명과학연구 (Journal of Agriculture & Life Science)|제47권 제1호|pp. 75-81

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참고문헌

키워드

Oxidative stress호박항산화효소산화적 스트레스Antioxidant enzyme활성산소종흰가루병Powdery mildewActive oxygen speciesCucurbita plants

초록

록흰가루병은 호박 시설재배 시 가장 큰 문제가 되는 병원균 중의 하나이다. 본 실험은 흰가루병 발병에 의한 산화적 스트레스가 항산화효소의 활성에 미치는 영향을 분석하였다. 흰가루병 발병과 더불어 과산화수소와 환원산소종의 생성은 급격하게 증가하였다. SOD와 HPX 밴드 패턴은 서양계 호박과 페포계 호박간에 다르게 나타났지만, 겔 활성은 흰가루병이 발병된 잎에서 높았다. 특히 서양계 호박에 있어서 relative mobility(Rm)이 0.46과 0.55인 밴드는 발병된 잎에서만 존재하였다. APX 밴드 패턴은 서양계 호박과 페포계 호박간에 차이가 없었으며 발병된 잎에서 활성이 높은 것으로 분석되었다.

Abstract

Powdery mildew (Sphaerotheca fuliginea Pollacci) is one of the most serious fungal diseases encountered in greenhouse-grown Cucurbita plants. To determine whether antioxidant enzyme systems were related to induction of oxidative stress induced by infection of powdery mildew. The initial response to infection of powdery mildew in the leaves of C. plants was generation of hydrogen peroxide and superoxide anions. Although the overall banding patterns of SOD and HPX were different between the C. maxima and pepo plants, the band profiles were characterized by high densities in the infected leaves. In the infected leaves of C. maxima, two major isozymes of HPX at the relative mobility (Rm) values of 0.46 and 0.55 were newly expressed at 20 days after inoculation. The overall banding patterns of APX were similar in the C. maxima and pepo plants. The gel activities of APX were activated in the infected leaves.

참고문헌 (31)

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