스트레스 관련 화합물 처리 및 병원균 감염에 의한 고구마 산성 퍼옥시다제 swpa2의 발현 유도
Induction of a Sweetpotato Anion Peroxidase swpa2 Gene Expression by Stress-related Chemicals and Pectobacterium chrysanthemi
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초록
스트레스 관련 화합물 및 병원균을 고구마 (Ipomoea batatas) 잎에 처리하여 고구마 배양세포에서 분리한 산성 퍼옥 시다제 swpa2 유전자의 발현을 RT-PCR로 분석하였다. Swpa2 유전자는 건강한 식물체 조직에서는 발현되지 않았으 나 각종 스트레스에 의해 발현이 유도되었다. Swpa2는 과산 화수소 (440 mM) 처리에 의하여 강하게 발현이 유도되었고, NaCl (100 mM), ABA (0.1 mM), methyl jasmonate (MeJA, 0.1 mM) 처리에 의하여도 발현이 유도되었다. 그러나 salicylic acid (SA) 처리에 의해서는 발현이 유도되지 않아, 이 유전자 는 SA와 MeJA의 신호전달에는 서로 다르게 관여함이 시사 되었다. 또한 swpa2 유전자는 무름병을 유발하는 박테리아 (Pectobacterium. chrysanthemii)의 감염에 의하여 강하게 발현 되므로, 이 POD는 병저항성에 관여함을 알 수 있었다. 따라서 swpa2 유전자는 각종 비생물학적 스트레스 뿐 아니라 생물학 적 스트레스에 수반되는 산화 스트레스를 극복하는데 관여함 이 강하게 제시되었다.
Abstract
Expression of an anionic peroxidase swpa2 gene isolated from cultured cells of sweetpotato (Ipomoeabatatas) was investigated under various stress conditions by RT-PCR. The swpa2 gene was not expressed in anytissues of intact sweetpotato plant grown at the normal condition. The expression of this gene was strongly inducedin leaf tissue by treatment of H2O2 (440 mM). Treatment of NaCl (100 mM), ABA (0.1 mM) and methyl jasmonate(MeJA, 0.1 mM) also induced the expression of swpa2 gene. Interestingly, salicylic acid (SA, 0.1 mM) did notinduce the expression of swpa2 gene, indicating that anionic swpa2 POD is differently involved in SA and MeJAsignaling pathways. In addition, swpa2 gene was strongly induced in sweetpoato leaf tisses infected withPectobacterium chrysanthemi, indicating that swpa2 is involved in defense related to the pathogenesis of P.chrysanthemi in sweetpotato plants. These results strongly suggest that swpa2 gene is involved in overcomingoxidative stresses caused by both abiotic and biotic stress.
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