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Different oxidative burst patterns occur during host and nonhost resistance responses triggered by Xanthomonas campestris in pepper

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초록

The hypersensitive reaction (HR) is the mostcommon plant defense reaction against pathogens. HR isproduced during both host- and nonhost-incompatible interactions. Several reports suggest that similarities existbetween host and nonhost resistances. We assayed the patternof generation of reactive oxygen species (ROS) and scavengingenzyme activities during nonhost pathogen-plantinteractions (Xanthomonas campestris pv. campestris/Capsicumannuum L.) and incompatible host pathogen-plant interactions(Xanthomonas campestris pv. vesicatoria race1/Capsicumannuum L.). Both O2- and H2O2 accumulated much fasterduring nonhost resistance when compared to the hostresistance. The scavenging enzyme activities of superoxidedismutase (SOD), catalase (CAT) and peroxidase (POX)were also different during the host- and nonhost-incompatibleinteractions. CAT activity was much higher during nonhost resistance, and several new isozymes of SOD and POX weredetected during nonhost resistance when compared to thehost resistance. Lipoxygenase (LOX) activity was higher inhost resistance than nonhost resistance during the earlystages of infection. Interestingly, the nitric oxide (NO) radicalaccumulated equal amounts during both host and nonhostresistance at early stages of infection. Further studies areneeded to determine the specific pathways underlying thesedifferences between host and nonhost resistance responses.

Abstract

The hypersensitive reaction (HR) is the mostcommon plant defense reaction against pathogens. HR isproduced during both host- and nonhost-incompatible interactions. Several reports suggest that similarities existbetween host and nonhost resistances. We assayed the patternof generation of reactive oxygen species (ROS) and scavengingenzyme activities during nonhost pathogen-plantinteractions (Xanthomonas campestris pv. campestris/Capsicumannuum L.) and incompatible host pathogen-plant interactions(Xanthomonas campestris pv. vesicatoria race1/Capsicumannuum L.). Both O2- and H2O2 accumulated much fasterduring nonhost resistance when compared to the hostresistance. The scavenging enzyme activities of superoxidedismutase (SOD), catalase (CAT) and peroxidase (POX)were also different during the host- and nonhost-incompatibleinteractions. CAT activity was much higher during nonhost resistance, and several new isozymes of SOD and POX weredetected during nonhost resistance when compared to thehost resistance. Lipoxygenase (LOX) activity was higher inhost resistance than nonhost resistance during the earlystages of infection. Interestingly, the nitric oxide (NO) radicalaccumulated equal amounts during both host and nonhostresistance at early stages of infection. Further studies areneeded to determine the specific pathways underlying thesedifferences between host and nonhost resistance responses.

참고문헌 (52)

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