어분대체 단백질원으로서 발효대두박이 넙치의 성장 및 육질에 미치는 영향
Effect of Extruded Pellets Containing Fermental Soybean Meal as a Partial Substitute for Fish Meal on Growth Performance and Muscle Quality of Olive Flounder(Paralichthys olivaceus)
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본 연구는 고온미생물인 Bacillus coagulans를 이용하여 발효시킨 탈피대두박(fermented soybean meal; FS)을 어분대체 단백질원으로서 10%, 20% 및 30% 수준별로 첨가한 배합사료를 급여하여 넙치의 성장 및 육질에 미치는 영향을 비교 조사하였다. 사육실험은 평균체중 123 g의 넙치를 원형수조에 50마리씩 실험구별로 3반복으로 수용하면서, 대조 배합사료(control)와 실험배합사료(FS10, FS20, FS30)를 8주간 사육하였다. 수분과 조단백질 함량은 대조 배합사료를 공급한 넙치의 등근육과 실험 배합사료를 공급한 넙치의 등근육에서 유의적한 차이를 보이지 않았으나, 조지방 함량은 실험 배합사료 FS20, FS30을 공급한 넙치 등근육에서 유의적으로 높은 값을 나타내었다. 18:2n-6의 함량은 실험배합사료 FS10, FS20, FS30을 공급한 넙치 등근육에서 대조 배합사료를 공급한 넙치 등근육보다 유의적으로 높게 나타났다. 모든 시험구에서 넙치 등근육의 유리아미노산 중 taurine, leucine, alanine, lysine이 공통적으로 높은 함량을 나타내었고, 공급한 사료에 따라 넙치 등근육의 유리아미노산에는 유의적인 차이는 없었다. 핵산관련물질 중 AMP의 함량은, FS20 및 FS30을 공급한 넙치 등근육에서 대조 배합사료를 공급한 것보다 유의적으로 높은 함량을 나타내었다. 이상의 결과로부터, 본 실험 사료조성으로 넙치 치어를 사육하였을 때 어분대체 단백질원으로서 발효대두박을 어분 단백질 함량 기준으로 30%까지 대체 가능할 것으로 생각되었다.
Abstract
This present study was conducted to evaluate the effect of fermented soybean meal(FS) as a partial substitute for fish meal on the growth and muscle quality of olive flounder (Paralichthys olivaceus). Three replicate groups of 50 fish per each tank (initial mean weight 123±0.5 g) were fed one of four experimental extruded pellets containing 0%, 10%, 20% and 30% fermented soybean meal (control, FS10, FS20 and FS30) for 8 weeks. No significant differences were found among fish fed the control, FS10, FS20 and FS30 in weight gain, feed efficiency and protein efficiency ratio (p<0.05). Crude fat content of fish fed FS20 and FS30 were significantly higher than that of fish fed the control. Significantly higher contents of 18:2n-6 was observed in fish fed FS10, FS20 and FS30 compared with fish fed the control (p<0.05). Polyunsaturated fatty acid content significantly increased with increasing dietary fermented soybean meal level (p<0.05). Most of free amino acid were composed of taurine, leucine, alanine and lysine but the differences were not significant (p<0.05). Adenosine monophosphate contents of fish fed FS20 and FS30 were significantly higher than that of fish fed control diet (p<0.05). In conclusion, fermented soybean meal can be used as a partial substitute for fish meal up to 30% in the diet without affecting growth performance in olive flounder.
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