만성 알코올 섭취로부터 유도되는 뇌 및 간 조직 독성에 대한 지구자 혼합추출물의 보호 효과
Protective effect of mixed extract including Hovenia dulcis Thunberg against chronic ethanol treatment-induced cytotoxicity in a brain and liver tissue
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산업화를 위한 지구자 및 12가지 식물성 원료 추출물의 생리학적 효과를 평가하기 위하여, 만성 에탄올을 투여한 마우스 혈청 내에서의 생리학적 지표 및 간과 뇌 조직 내에서의 산화적 스트레스에 대한보호효과를 확인하였다. 에탄올을 투여한 마우스의 혈당은 정상 대조군 그룹(NG)과 에탄올 투여 그룹(EG)에서 각각 119.43mg/dL, 305.25mg/dL로 나타났고, 에탄올과 혼합 추출물을 동시에 투여한 그룹(100, 200mg/kg body weight + 25% ethanol 5g/kg body weight, ME100, ME200)에서 각각272.76mg/dL, 234.60mg/dL로 감소하였다. 혈중 에탄올 함량은 EG에서 4.08mg/dL를 나타내었고ME100, 200에서 각각 3.85mg/dL, 3.08mg/dL로 감소하였으며, 혈중 아세트알데하이드 함량은18.72mg/dL에서 각각 15.76mg/dL, 15.16mg/dL로 감소하였다. 또한 ME100, ME200은 혈청 내의 생리학적 지표에서 간 독성 지표인 glutamine pyruvic transaminase(GPT), glutamic oxaloacetictransaminase(GOT)와 신장 독성 지표인 blood urea nitrogen(BUN), creatine(CRE), 혈중 totalcholesterol(TCHO), triglyceride(TG)의 함량이 유의하게 감소하였다. 뇌 조직에서 에탄올에 의해acetylcholinesterase(AChE)가 EG(116.10%)에서 NG(100.00%)와 비교하였을 때 증가된 활성을 나타냈으나, ME에서 각각 109.00%와 108.47%로 유의적으로 감소하였다. ME에서 EG에 비해 간과 뇌 조직에서 superoxide dismutase(SOD)의 함량이 증가하였고, oxidized glutathione(GSH)/total GSH 비율과malondialdehyde(MDA)의 함량이 감소하였다. 이러한 결과들을 통해 지구자를 포함한 혼합 추출물은간, 뇌 조직 및 혈액 등에서 만성 에탄올 섭취에 의해 유발된 산화적 스트레스를 효과적으로 보호할 수있는 제품으로의 개발이 가능할 것으로 판단된다.
Abstract
To assess the industrial possibility of mixed-extracts containing Hovenia dulcis Thunberg and12 different botanical ingredients, a protective effect was confirmed in the chronicethanol-induced the liver, brain, and blood injury in mouse. Blood glucose levels of the normalcontrol group(NG) and ethanol administration group(EG) were respectively 119.43mg/dL and305.25mg/dL, and the mixed-extracts administration group(100, 200mg/kg body weight + 25%ethanol 5g/kg body weight respectively; ME100 & ME200) were decreased to 272.76mg/dL and 234.60mg/dL. Blood ethanol contents were decreased in ME100 and ME200(3.85mg/dL,3.08mg/dL) compared to EG(4.08mg/dL), and blood acetaldehyde contents were also decreasedin ME(15.76mg/dL, 15.16mg/dL) compared to EG(18.72mg/dL). The contents of hepatotoxicindicators such as glutamine pyruvic transaminase(GPT) and glutamic oxaloacetic transaminase(GOT), nephrotoxic indicators such as blood urea nitrogen(BUN), and creatine(CRE), and totalcholestero(TCHO), and triglyceride(TG) in mouse blood serum were significantly decreased inthe ME compared to EG. The acetylcholinesterase(AChE) activity of ME(109.00% and 108.47%,respectively) in mouse brain tissues was decreased in ME compared to EG(116.10%). Finally,ME was remarkable in vivo antioxidant activities in the mouse liver and brain tissues bysuperoxide dismutase(SOD), oxidized glutathione(GSH)/total GSH ratio and the malondialdehyde(MDA) assay. Therefore, the mixed-extracts was considered to be effective a high value foodwith protective effect against chronic ethanol traetment-induced cytotoxicity in liver and braintissues.
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