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만성 알코올 처리 쥐에 대한 헛개나무 열매 농축액을 함유한 콩나물 혼합물의 숙취해소 및 간 기능 개선 효과

Effects of a soybean sprouts mixture containing Hovenia dulcis Thunb. fruit concentrate on hangover relief and liver function improvement in chronically alcohol-treated rats

김정호|원영선|박울림|민혜지|서권일|허지안
한국식품저장유통학회 (The Korean Society of Food Preservation)|2024.06|Food Science and Preservation|제31권 제3호|pp. 486-498|DOI 10.11002/fsp.2024.31.3.486

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참고문헌

키워드

alcoholoxidative stresslipid accumulationsoybean sproutsHovenia dulcis Thunb.
주제분류식품과학

초록

본 연구는 만성 알코올 유도 쥐에 대한 1.5% 헛개나무 열매 농축액이 첨가된 콩나물 혼합물의 숙취해소 및 간 기능 개선 효과를 확인하고자 하였다. 헛개나무 열매 농축액을 함유한 콩나물 혼합물군(SHM) 내 ampelopsin 및 L-asparagine의 함량은 10.52, 35.19 ppm으로 확인되었으며, 만성 알코올 급여에 의해 체중 변화량이 감소하고, 간 중량은 증가한 반면 SHM 처리시 유의적으로 체중 변화량이 증가하였으며, 간 중량이 감소하였다. 혈중 alcohol 및 acetaldehyde의 농도는 SHM군에서 2.89, 0.53 g/L로 가장 낮은 함량을 보였으며, 간 조직 ADH 및 ALDH의 활성은 SHM군에서 33.11, 102.06 U/L로 가장 높은 활성을 나타냈다. 만성 알코올 유도는 ALT, AST 및 GGT와 같은 간 기능 지표 효소들의 활성을 증가시켰지만, SHM 처리시 각각 41.00, 130.83 및 0.47 U/L로 활성이 유의적으로 감소하였다. 간 조직 및 혈중 triglyceride 함량은 SHM군에서 1.45와 33.00 mmol/L로 가장 낮은 값으로 나타났고, 만성 알코올 투여로 증가된 혈중 total cholesterol 및 LDL cholesterol 함량은 SHM에 의해 감소하였으며, 만성 알코올 투여로 인해 감소된 혈중 HDL cholesterol 함량은 SHM 처리시 증가하였다. 간 조직을 형태학적 및 병리학적으로 관찰한 결과, 만성 알코올 유도군의 간 조직은 형태학적 관찰에서 황갈색을 띠었고, 병리학적 관찰에서는 지질방울의 크기 및 수가 증가하였다. 그러나 SHM 처리시 형태학적으로 관찰한 간 조직의 색은 적갈색으로 나타났고, 병리학적으로 관찰한 간 조직의 지질방울 크기 및 수는 감소하였다. SHM군의 간 조직 및 혈중 지질 과산화 함량은 677.68, 76.26 nmol/g으로 가장 낮은 값으로 확인되었으며, 간 조직 및 혈중 GSH 함량은 SHM군에서 가장 높은 값인 19.93 및 20.08 μmol/kg으로 나타났다. 따라서 콩나물 또는 헛개나무 열매의 단일 섭취보다 혼합하였을 때 ampelopsin 및 asparagine과 같은 유효성분들의 시너지에 의해 SHM군에서 증진된 숙취해소 및 간 기능 개선 효과를 확인할 수 있었으며, 이는 기능성 식품 소재화에 대한 가능성을 제시하는 기초 자료로 활용될 수 있음을 시사한다.

Abstract

This study examined the effects of a soybean sprouts mixture containing 1.5% Hovenia dulcis Thunb. fruit concentrate (SHM) on relieving hangovers and improving liver function in chronically alcohol-treated rats. The ampelopsin and L-asparagine contents in the SHM were 10.52, 35.19 ppm. When chronic alcohol-induced rats were treated with SHM, the body weight increased and the liver weight decreased. The serum alcohol and acetaldehyde concentrations were lowest in the SHM group, and the hepatic ADH and ALDH activities were highest in the SHM group. Chronic alcohol induction increased the activity of liver function indicator enzymes such as ALT, AST, and GGT, but the activity was decreased significantly with the SHM treatment. The triglyceride content in hepatic and serum blood samples was lowest in the SHM group. The serum total cholesterol and LDL cholesterol contents decreased in the SHM group, and the HDL cholesterol content increased. The color of the hepatic observed morphologically in the SHM group was reddish brown, and the size and number of lipid droplets in the hepatic observed pathologically decreased. The hepatic and serum lipid peroxidation content of the SHM group decreased. The hepatic and serum GSH content increased in the SHM group. Therefore, SHM can be a functional food that can help hangovers and improve liver function.

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