단위동물 모델에서 In vitro 소화율 측정과 평가
Evaluation and Method of In Vitro Digestibility in Monogastric Animal Model
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초록
In vivo 측정법은 비용이 많이 들고 연구기간이 길며 동물복지 측면에서 여러 문제점이 있다. 이에대한 대안으로 지난 수십년간 in vitro 소화율 측정법이 개발되어 왔다. 본 총설에서는 대표적인 단위동물로써 사양되고 있는 돼지의 소화기관 특징 및 소화작용에 대한 논하였다. 또한, 최근에 개발된daisy II incubator 를 이용한 in vitro 방법을 통해 돼지에서 주요 곡류사료로 이용되고 있는 옥수수,쌀, 소맥 및 대맥의 회장 및 전분 건물소화율을 측정하였다.
Abstract
An in vitro digestibility methods have been developed to mimic an in vivo system in the pastdecades. Because the in vivo techniques cause high cost, intensive labor, longer research periodsand ethnical problems. In this review, the digestive systems from stomach to large intestine ofpig as a monogastric animal were addressed to understand an in vivo digestion. The innovativein vitro technique using the Daisy II incubator was performed and the in vitro ileal and fecaldry matter (DM) digestibility of corn, rice, wheat and barley was determined.
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