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체외 소화과정 동안 Gastric lipase의 첨가가 반려견 사료의 소화율에 미치는 영향

Effects of Gastric Lipase on the Digestibility of Dog Food during in vitro Digestion

이민영|김기현|조현우|김찬호|정소희|임세진|양가영|전중환|천주란|서강민
농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)|2023|농업생명과학연구 (Journal of Agriculture & Life Science)|제57권 제3호|pp. 123-128

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키워드

pH반려견 사료정적 체외 소화모델지방 소화율Gastric lipaseDog foodFat digestibilityStatic in vitro digestion model

초록

본 연구는 위장 단계의 소화과정에 관여하는 Gastric lipase (GL)를 반려견을 위한 정적 체외 소화모델(Static in vitro digestion model)에적용을 검토하기 위하여 실시되었다. GL의 첨가가 체외 소화과정 동안 건물(Dry matter; DM), 조단백질(Crude protein; CP) 그리고 조지방(Etherextracts; EE) 소화율에 미치는 영향을 평가하였다. GL은 위장 소화단계에서 첨가되었다. 위장(39℃, 2 hr.)과 소장(39℃, 4 hr.) 소화 후에 비소화분획을 분리하였다. 그리고 실험사료와 분리된 비소화 분획에서 DM, CP 그리고 EE 수준을 측정하고 각각의 소화율을 계산하였다. 위장과 소장소화단계에서 측정된 DM, CP 그리고 EE 소화율은 Control과 GL 그룹 사이에서 통계적으로 유의한 차이는 관찰되지 않았다(p>0.05). 결과적으로우리의 체외 소화모델에서 GL의 첨가는 DM, CP 그리고 EE의 소화율에는 영향을 미치지 않는 것으로 나타났다. 따라서 이와 같은 결과는 정적체외 소화모델을 이용한 소화율의 평가에 있어서 GL의 역할은 다소 제한적일 수 있다는 것을 시사한다.

Abstract

The effect of gastric lipase (GL) on the digestibility of dry matter (DM), crude protein (CP) and ether extracts (EE) was investigatedduring in vitro digestion as a process to optimize the condition of a static in vitro digestion model for dogs. GL was added duringthe gastric phase of in vitro digestion. After gastric (39℃, 2 hr.) and intestinal (39℃, 4 hr.) digestion phases, the non-digested fractionwas collected from the final reactant DM, CP, and EE levels in the non-digestible fraction were analyzed and those of the digestibilitywas calculated. DM, CP and EE digestibility at gastric and intestinal digestion phases were not significantly different between the Controland GL groups (p>0.05). As a result, in the static in vitro digestion model, the addition of GL did not affect the digestibility of DM,CP and EE. Therefore, these results suggest that the role of GL in the evaluation of digestibility of dog`s food using a static in vitrodigestion model are limited.

참고문헌 (29)

  1. Alvarenga CI, "The effect of increasing levels of dehulled faba beans (Vicia faba L.) on extrusion and product parameters for dry expanded dog food", Foods, 8, pp.26~ (2019)[DOI]
  2. Armand M, "Lipases and lipolysis in the human digestive tract: Where do we stand?", Curr. Opin. Clin. Nutr. Metab. Care, 10, pp.156~164 (2007)[DOI]
  3. Association of Official Analytical Chemists(AOAC), "Official methods of analysis of AOAC international" (2006), AOAC International
  4. Bakala-N’Goma JC, "Toward the establishment of standardized in vitro tests for lipid-based formulations. 5. Lipolysis of representative formulations by gastric lipase", Pharm. Res, 32, pp.1279~1287 (2015)[DOI]
  5. Biagi G, "A new in vitro method to evaluate digestibility of commercial diets for dogs", Ital. J. Anim. Sci, 15, pp.617~625 (2016)[DOI]
  6. Biehler E, "Methods for assessing aspects of carotenoid bioavailability", Curr. Nutr. Food Sci, 6, pp.44~69 (2010)[DOI]
  7. Boisen S, "Prediction of the apparent ileal digestibility of protein and amino acids in feedstuffs and feed mixtures for pigs by in vitro analyses", Anim. Feed Sci. Technol, 51, pp.29~43 (1995)[DOI]
  8. Carriere F, "Secretion and contribution to lipolysis of gastric and pancreatic lipases during a test meal in humans", Gastroenterology, 105, pp.876~888 (1993)[DOI]
  9. Carriere F, "Gastric and pancreatic lipase levels during a test meal in dogs", Scand. J. Gastroenterol, 28, pp.443~454 (1993)[DOI]
  10. Carriere F, "Purification and biochemical characterization of dog gastric lipase", Eur. J. Biochem, 202, pp.75~83 (1991)[DOI]
  11. Carriere F, "Dog gastric lipase:Stimulation of its secretion in vivo and cytolocalization in mucous pit cells", Gastroenterology, 102, pp.1535~1545 (1992)[DOI]
  12. Gargouri Y, "Kinetic assay of human gastric lipase on short-and long-chain triacylglycerol emulsions", Gastroenterology, 91, pp.919~925 (1986)[DOI]
  13. Golding M, "The influence of emulsion structure and stability on lipid digestion", Curr. Opin. Colloid Interface Sci, 15, pp.90~101 (2010)[DOI]
  14. Hasjim J, "In vivo and in vitro starch digestion: Are current in vitro techniques adequate?", Biomacromolecules, 11, pp.3600~3608 (2010)[DOI]
  15. Heerup C, "Effects of recombinant human gastric lipase and pancreatin during in vitro pediatric gastro-intestinal digestion", Food Funct, 12, pp.2938~2949 (2021)[DOI]
  16. Larsson K, "Oxidation of cod liver oil during gastrointestinal in vitro digestion", J. Agric. Food Chem, 60, pp.7556~7564 (2012)[DOI]
  17. Lorrain B, "Dietary iron-initiated lipid oxidation and its inhibition by polyphenols in gastric conditions", J. Agric. Food Chem, 60, pp.9074~9081 (2012)[DOI]
  18. Maldonado-Valderrama J, "In vitro gastric digestion of interfacial protein structures: Visualisation by AFM", Soft Matter, 6, pp.4908~4915 (2010)[DOI]
  19. McCusker S, "Amino acid content of selected plant, algae and insect species:A search for alternative protein sources for use in pet foods", J. Nutr. Sci, 3, pp.e39~ (2014)[DOI]
  20. Ménard O, "Validation of a new in vitro dynamic system to simulate infant digestion", Food Chem, 145, pp.1039~1045 (2014)[DOI]
  21. Minekus M, "A standardised static in vitro digestion method suitable for food–an international consensus", Food Funct, 5, pp.1113~1124 (2014)[DOI]
  22. Sams L, "Relevant pH and lipase for in vitro models of gastric digestion", Food Funct, 7, pp.30~45 (2016)[DOI]
  23. Sassene PJ, "Comparison of lipases for in vitro models of gastric digestion: Lipolysis using two infant formulas as model substrates", Food Funct, 7, pp.3989~3998 (2016)[DOI]
  24. Seo K, "Influence of bile salts and pancreatin on dog food during static in vitro simulation to mimic in vivo digestion", Animals, 12, pp.2734~ (2022)[DOI]
  25. Theysgeur S, "New bioactive peptides identified from a tilapia byproduct hydrolysate exerting effects on DPP-IV activity and intestinal hormones regulation after canine gastrointestinal simulated digestion", Molecules, 26, pp.136~ (2020)[DOI]
  26. Ville E, "Physiological study of pH stability and sensitivity to pepsin of human gastric lipase", Digestion, 65, pp.73~81 (2002)[DOI]
  27. Volhard F, "Über das fettspaltende ferment des magens", Z. Klin. Med, 42, pp.414~429 (1901)
  28. Vors C, "Coupling in vitro gastrointestinal lipolysis and Caco-2 cell cultures for testing the absorption of different food emulsions", Food Funct, 3, pp.537~546 (2012)[DOI]
  29. Wickham M, "In vitro digestion methods for assessing the effect of food structure on allergen breakdown", Mol. Nutr. Food Res, 53, pp.952~958 (2009)[DOI]

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