쌀, 보리, 옥수수의 반추위내 In situ 및 In vitro 분해율, 미생물 성장과 Gas 발생량에 대한 연구
Studies on In situ and In vitro Degadabilities, Microbial Growth and Gas Production of Rice, Barley and Corn
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본 시험은 반추위 누관이 시술된 한우 수소 3두를 이용하여 쌀, 옥수수 및 보리의 in situ 분해율과 in vitro 시험으로서 영양소 분해율, 미생물 성장량, pH 변화 및 gas 발생량을 비교분석함으로써 쌀의 사료적 이용성을 평가해 보고자 수행되었다.In situ 건물 및 유기물 소실율은 반추위내 24시간 발효 시, 보리와 쌀은 거의 대부분이 소실된 반면, 옥수수는 약 67%만 소실되어 분해 속도가 매우 느린 것으로 나타났다. 수용성 물질인 ‘a’ 부분은 옥수수, 쌀, 보리 순으로 많았으며, 천천히 분해되는 ‘b’ 부분은 ‘a’ 부분과 정 반대의 경향으로서 ‘b’ 부분의 분해 상수(‘c’ 부분)로부터 시간당 건물과 유기물의 분해속도는 보리의 경우 각각 38.3%, 37.2%로서 쌀(7.7%, 5.6%)이나 옥수수(4.1%, 1.3%)에 비해 매우 빠르게 일어났다. In vitro 건물 및 유기물 분해율은 in situ 분해율에 비해 수준은 현저히 낮았으나, 보리, 쌀, 옥수수 순으로 높아 곡류 간 순서는 일치하였다. In vitro 미생물 성장은 쌀이 옥수수나 보리보다 상대적으로 낮았으나, 가스 발생량은 높게 나타났으며, pH는 발효시간에 따른 저하속도가 쌀에서 가장 느린 것으로 나타났다. 발효시간에 따른 미생물 성장량은 발효 12시간까지 높아졌다가 이후로 감소하였고, 배양액의 pH는 발효초기에는 높았다가 이후로 점차 감소하였으며, gas 발생량은 발효 9시간까지는 빠르게 증가하였다가 이후로 증가폭이 둔화되었다.이상의 결과에서 반추위내 건물과 유기물의 분해율과 발효시간의 경과에 따른 pH의 저하속도 등을 고려하면, 쌀은 옥수수와 함께 반추위내 발효 안정을 위한 에너지원으로서 사용 할 수 있을 것으로 사료된다.
Abstract
Ground rice, barley and corn were fed separately to the ruminally cannulated Hanwoo (Korean native cattle) for comparing their in situ and in vitro degradabilities, microbial growth, pH and gas production. It has been found that nearly all the dry matter (DM) and organic matter (OM) in barley and rice disappeared during 24 hr suspension in the rumen, but those in corn were only reduced by around 67%. Water soluble DM and OM fractions(‘a’), ranked from highest to lowest was corn, then rice and finally barley, but the order was reversed for content ‘b’, degradable fraction during time ‘t’. Judging by the degradation parameter of ‘b’ fraction, degradation rates per hour of DM and OM for barley were 38.3% and 37.2% respectively, significantly higher than those for rice (7.7% and 5.6%) and corn (4.1% and 1.3%). In general, results obtained from in vitro degradability of DM and OM were lower than those from in situ trials, but the ranking order of degradability was in agreement between both trials. In particular, ground rice has relatively lower in vitro microbial growth than corn or barley, but exhibited higher gas production. In addition, in vitro microbial growth of ground rice increased with up to 12 hr of incubation period, thereafter experienced a decrease with extended incubation time. pH of in vitro solution of rice decreased following 9 hr of incubation but gas production increased rapidly during the same period. From the results of DM and OM degradabilities and pH changes of in vitro solution with incubation time, it is concluded that rice represents a good source of energy for stability of rumen fermentation.
참고문헌 (33)
- "Influences on in vitro digestibility" (1977)
- "Official methods of analytical chemists" (1990)
- "The hydrodynamic behaviour of native amylose Conformation of Biopolymers" (1975), Academic Press
- "Prediction of the amount of methane produced by ruminants", 19, pp.511~ (1965)
- "Energy metabolism in animal and man" (1989), Cambridge University press
- "Degradation of starch by incubation with rumen fluid comparison of different starch sources" (1989)
- "Extent of cellulose and hemicellulose digestion in various forage by pure cultures of rumen bacteria", 50, pp.1136~ (1967)
- "The influence of the starch and sugar content of concentrations on feed intake production and composition of milk Proceedings of Disease farm animals" (1980)
- "A simple apparatus for measuring gas production by methanogenic cultures in serum bottles", 4, pp.425~ (1983)
- "Effect of roasting row soybeans at three temperatures on in situ dry matter and nitrogen disaddearance in dairy cows" (1990)
- "Corn particle size and site and extent of digestion by steers" (1979)
- "Effect of feeding whole and rolled barley to steer in the morning or afternoon in diets containing different proportions of hay and grain", 53, pp.205~ (1991)
- "Methane production in dairy cows", 62, pp.1583~ (1979)
- "Procedure for two-stage in vitro digestion of forage Nutrition research technique for domestic and wild animals" (1970)
- "Associative effects of mixed feeds effects of type and level of supplementation and the influence of the rumen fluid pH on cellulolysis in vivo and dry matter digestion of various roughages" (1984)
- "Nutrient requirements of domestic animals Nutient requirements of beef cattle" (1984)
- "In situ and other methods to estimate ruminal protin and energy digestibility" (1988)
- "Digestibility studies with cows given whole and rolled cereal grains" (1976)
- "Control of digestion and metabolism in ruminants", Prentice-Hall
- "Influence of ammoniation of high moisture barley on digestibility kinetics of rumen ingesta turnover and milk production in dairy cows" (1989)
- "Factors affecting starch digestibility with special emphasis on sorghum and corn", 63, pp.1607~ (1986)
- "The technology of sorghum products Recent progress in cereal chemistry Cereals for Food and Beverages", pp.515~554 (1980), Academic Press
- "User's Guide: Statistics." (1997), Statistical Analysis System. Inst. Inc.,
- "Methane production in heifers sheep and goats consuming diets of various hay-concentrate ratios" (1992)
- "The effect of particle size and steam treatment of feedstuffs on rate and extent of digestion In vitro and in situ" (1988)
- "Study of a nylon bag technique for in vivo estimation of froage digestibility", sci 21, pp.340~ (1962)
- "Nutritional Ecology of the Ruminants", pp.106~108 (1982), INC
- "Advances in Cereal Science and Technology Ⅱ", pp.135~219 (1978)
- "Molecular structure of starch", pp.153~182 (1984), Academic Press
- "농림업 주요 통계" (2002), 농림부
- "곡류사료수출입통계" (2003), 농림부
- "한우 육성 비육 시 보리 급여수준이 증체와 육질에 미치는 효과", 48(2), pp.247~ (2006)
- "옥수수의 가공처리가 영양소 이용성에 미치는 영향 젖소 반추위내에서 건물 및 조단백질의 소실률과 분해도", 채택일자：2006 (1994), ~