Eucalyptus pellita의 절편 위치에 따른 부정아유도
Induction of Adventitious Shoot by Plant Explant Position of Eucalyptus pellita
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초록
Eucalyptus pellita의 유엽을 재료로 효율적인 재분화 시스템을 확립시켰다. 부정줄기는 MS배지에1.0㎎l-1 benzyl aminopurine(BA)과 1.0㎎l-1 naphthalene acetic acid (NAA)가 첨가된 배지의 유엽에서 유도되었다. 그리고 절편의 종류와 위치에 따라 다양한 재분화 반응을 보였다. Auxin과 cytokinin이 혼합 첨가된 배지에서 엽병이 포함된 유엽의 잎 절편(LP)은 잎 끝이 포함된 유엽(LT)의 절편과 비교하여 더 높은 재분화율을 보여주었다. 줄기와 뿌리의 길이신장은 호르몬이 무첨가된 1/2 Woody PlantMedium(WPM)배지에서 이루어 졌다. 이렇게 유도된 식물체는 성공적으로 온실에 이식되었다.
Abstract
An effficient regeneration system has been developed from young- leaf of Eucalyptus pellita. Adventitious shoots were induced using young-leaf explants cultured on Murashige and Skoogmedium containing 3% scurose, 0.3% agar and supplemented with 1.0㎎l-1 benzyl aminopurine(BA)and 1.0㎎l-1 naphthalene acetic acid(NAA). And the intensity of the regeneration response variedaccordingly to the explants type and orientation. Explants with petiole of leaflet(LP) showed thehighest regeneration rate compared to explatns with leaf tip of leaflet(LT), in the auxin andcytokinin combination used. Shoot and root elongation were achived on half-Woody PlantMedium(WPM) without plant growth regulator. Plantlets were then successfully transplanted togreenhouse conditions.
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