번행초의 잎 절편으로부터 신초의 재분화
Shoot Regeneration from the Leaf Explants of Tetragonia tetragonoides O.KUNTZE
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초록
A protocol has been developed for differentiation of adventitious shoots directly from leaf segments of Tetragonia tertragonoides O. Kuntze. Murashige and Skoog (MA) medium supplemented with 2mg/L N6-benzyladenine (BA) and 0.5mg/L α- naphthaleneacetic acid (NAA) supported the induction of adbentitious shoots from leaf explants. Adventitious shoots were multiplied by subculturing on the double strength MS (2MS) medium supplemented with 0.5mg/L NAA and 2 mg/L BA. Shoots were rooted on MS basal medium without any growth regulators.
Abstract
A protocol has been developed for differentiation of adventitious shoots directly from leaf segments of Tetragonia tertragonoides O. Kuntze. Murashige and Skoog (MA) medium supplemented with 2mg/L N6-benzyladenine (BA) and 0.5mg/L α- naphthaleneacetic acid (NAA) supported the induction of adbentitious shoots from leaf explants. Adventitious shoots were multiplied by subculturing on the double strength MS (2MS) medium supplemented with 0.5mg/L NAA and 2 mg/L BA. Shoots were rooted on MS basal medium without any growth regulators.