Eucalyptus pellita 기내발아묘의 부정아 유도에 미치는 BA 및 NAA 효과
Effect of BA and NAA on Adventitious Bud Induction In vitro Germinant Eucalyptus pellita
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부정아 유도를 통한 E. pellita의 재분화 체계를 확립하고자 기내 발아묘를 재료로 부정아 유도에 미치는 BA 및 NAA 효과를 시험하였다. 기내 부정아 유도는 재료의 노화 정도, 절편 및 생장조절제에 따른 차이가 큰 것으로 나타났다. 발아 후 7일된 식물체가 발아 후 14일 된 것 보다 양호한 반응을 보였고, 절편은 자엽 〉배축 〉뿌리 순으로 부정아 유도가 이루어졌다. 뿌리에서는 부정아가 전혀 유도되지 못했다. 적정배지는 MS 배지에 0.5 mg/L BA, 0.2 mg/L NAA로 나타났으며, 유도된 부정아는 DKW + BA 0.2 mg/L + NAA 0.01 mg/L 배지에서 정상적인 줄기로 재생 되었고, 다경으로 생장되고 자발적인 발근도 이루어 졌다. 이상의 결과는 어린 자엽과 배축에서 부정아 유도를 통해 E. pellita의 증식이 가능함을 보여주었다.
Abstract
This study was conducted to examine the effect of BA and NAA on adventitious bud induction fromin vitro germinants E. pellita. The capacity of adventitious bud formation greatly depends on juvenility and explants origin; the more juvenile materials are the better ability to form adventitious buds even in in vitro raised plantlets. In case of in vitro germinants, 7 day old plantlets showed a better morphological response than did 14 day old ones in the induction of adventitious buds. The capacity to show morphological response was in decreasing order : cotyledons> petioles> roots. No adventitious buds formed when root segments were used as culture material. And optimum medium appeared to be MS + 0.5 mg/L BA and 0.2 mg/L NA. Adventitious buds could be developed into multiple shoots and regenerated normal plantlets on DKW medium plus 0.2 mg/L BA and 0.01 mg/L NAA.
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