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천연기념물 제432호 제주 상효동 한란 집단내유전다양성 및 공간적 유전구조

Genetic Diversity and Spatial Structure of a Population the Natural Monument(No. 432) Cymbidium kanran in Sanghyo-dong, Jeju-do

조용찬|권혜진|손성원|신재권|김은혜|배관호
농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)|2015|농업생명과학연구 (Journal of Agriculture & Life Science)|제49권 제5호|pp. 1-11|DOI http://dx.doi.org/10.14397/jals.2015.49.5.1

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참고문헌

키워드

공간적 자기상관Endangered speciesSpatial autocorrelationGenetic variationPollinator멸종위기종수분매개충유전변이유전자흐름Gene flow

초록

본 연구는 한국 희귀종인 한란의 보전을 위하여 ISSR 표지자를 이용한 유전변이와 분화 및 공간적유전구조를 분석하였다. 분석된 유전다양성(Species level: h=0.303, S.I=0.389)은 근연종과 다른 희귀종에 비해 높은 유전변이를 나타내었다. 또한 유전자형 다양성(Species level: GN/N=0.884, D=0.996)역시 높게 나타나 주로 타가수정이 이루어지는 것으로 조사되었다. 한편, 소집단 A와 B 간에 분화정도는 23%로 소집단 간의 인접거리를 고려했을 때, 집단 내에서도 분포의 위치에 따라 분화가 발생하고있었다. 높은 수준의 유전다양성과 분화의 발생요인을 고려했을 때, 과거 외부에서 지속적으로 유입된한란 개체로부터의 영향과 매개충의 역할이 주요한 원인으로 추정되었다. 소집단의 공간적 유전구조 분석에서 A는 9m, B는 6m 이내에 분포하는 개체들 간에 유전적 유사성이 높은 것으로 나타났다. 이 결과를 바탕으로 한란의 현지 외 보전을 위한 표본 추출 시 최소 9m 이상의 간격을 유지하는 것을 제안하는 바이다.

Abstract

The genetic diversity and spatial structures of natural populations of Cymbidium kanran onJeju island were estimated using ISSR markers. The genetic diversity(species level: h = 0.303,S.I = 0.389) and genotypic diversity(species level: GN/N = 0.884, D = 0.996) of this plant werefound to be higher than in other rare species. AMOVA analysis, revealed that 23% of the totalgenetic variation was allocated between two sub-populations. Taking the high levels of geneticdiversity and causative factors for differentiation into consideration, the major causes wereassumed to be the influence of alien individuals of C. kanran continuously introduced fromoutside in the past and the role of the intermediate host. Spatial autocorrelation analysisrevealed significant spatial genetic structure within 9m and 6m distance in two sub-populationsrespectively. As a sampling strategy for ex-situ conservation of C. kanran, a minimum distanceof 9m between individuals is recommended.

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