한국 특산식물 매미꽃(Coreanomecon hylomeconoides Nakai) 집단의 유전다양성 및 구조
Genetic Diversity and Structure of the Korean Endemic Species, Coreanomecon hylomeconoides Nakai, as Revealed by ISSR markers
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초록
우리나라 특산식물인 매미꽃(Coreanomecon hylomeconoidesNakai) 집단의 유전적 다양성 및 구조를 조사하기 위해 8집단 224개체에 대한 ISSR(Inter SimpleSequence Repeat) 분석이 수행되었다. 총 8개의 ISSR 프라이머를 이용하여 50개의 증폭산물을 관찰하였으며 집단 수준에서의 유전적 다양성의 평균은 P (Percentage ofpolymorphic loci) = 47.3%, SI(Shannon’s informationindex) = 0.218, h (Nei’s genetic diversity) = 0.142로다년생 초본류의 평균보다는 월등히 낮게 나타났다. 집단별로는 분포의 중심에 해당하는 산청(SI=0.233, h=0153),광양(SI=0.263, h=0.171), 순천(SI=0.241, h=0.159) 집단이 남해(SI=0.183, h=0.116)나 광주(SI=0.181, h=0.121)의 변두리 집단보다는 비교적 높은 유전다양성을 유지하는것으로 나타났다. AMOVA 분석 결과 전체 유전변이의 약18%가 지역 간에 나머지 82%가 집단 내 개체간의 차이에기인하는 것으로 나타났는데 이는 집단 간에 유전자 교류가 원활히 이루어지기 때문으로 판단된다. 유전적 거리를이용한 UMGMA 유집분석과 Bayesian cluster 분석 결과,매미꽃 집단은 동서 두 지역으로 구조화 되는 경향을 보여주었는데 이는 집단의 지리적 분포 패턴의 영향인 것으로추정할 수 있다. 본 연구 결과, 조사된 다른 집단보다 풍부한 개체수와 높은 유전 다양성을 유지하고 있는 지리산 및백운산의 산청, 광양 집단들에 대한 적극적인 현지 내(insitu) 보전대책 수립이 요구된다.
Abstract
The genetic diversity and structure of eight populations of Coreanomecon hylomeconoides Nakai, an endemicKorean plant, were investigated using 50 ISSR loci from eight primers. The average percentage of polymorphic loci was47.3%. The Shannon’s index (SI=0.218) and gene diversity (h=0.142) were relatively lower than those of other long-livedperennials. The Sancheong (SI=0.233, h=0153), Gwangyang (SI=0.263, h=0.171), and Suncheon (SI=0.241, h=0.159)populations showed greater genetic diversity than the Namhae and Gwangju populations, which are on the edge of thedistribution. Analysis of molecular variance (AMOVA) showed that 18% of the total variation could be attributed todifferences among populations, and 82% to differences within populations, indicating moderate gene flow among adjacentpopulations. These results were supported by value of Nm (2.184). The UPGMA conducted using the genetic distance andBayesian cluster analysis showed a remarkable geographic trend structured into east and west regions. Overall, the resultsindicate that the Sancheong and Gwangyang populations, which had a large population size and higher degree of geneticdiversity, should be the focus of in situ conservation.
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