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장기간 기내 배양한 춘란(Cymbidium goeringii Lindley) 및 한란(Cymbidium kanran Makino)의 변이 비교

Variation Analysis of Long-term in vitro Cultured Cymbidium goeringii Lindley and Cymbidium kanran Makino

이효연|류재혁|배창휴
한국자원식물학회 (The Plant Resources Society Of Korea)|2011.04|한국자원식물학회지 (Korean Journal of Plant Reources.)|제24권 제2호|pp. 139-149

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키워드

RAPDGenetic similarity matrixSomaclonal variationC. goeringii LindleyC. kanran Makino

초록

기내 배양된 Cymbidium속 춘란과 한란의 근경을 대상으로 장기배양에 따른 변이성을 비교하기 위하여 RAPD 분석을 실시하였다. 춘란은 총 151개 DNA 밴드 중 40개의 다형성 밴드가 증폭되어 다형성 비율은 26.4%였으며, 한란은 총 155개 밴드 중 56개의 다형성 밴드가 증폭되어 36.1%의 다형성 비율을 나타내었다. 단순일치 계수(simple- matching coefficient)를 사용하여 유전적 유사도 지수를 분석한 결과, 춘란의 개체간의 유전적 유사도 지수는 0.825~ 1.00 사이로 평균 0.944였다. 한란의 개체간 유전적 유사도 지수는 0.812~1.00 사이로 평균 0.913이었다. 군집분석결과 춘란은 유전적 유사도 지수 0.841과 0.837에서 1개의 그룹과 유집되지 않는 2개체로 나누어졌으며 한란보다 단순하게 유집되었다. 이와 같이 생장조절제가 첨가된 기내 배지에서 장기간 배양된 Cymbidium 근경은 유전적 다형성을 나타냈으며, 유전적 유사지수도 다소 낮게 나타났다. 이 결과는 추후 여러 가지 변이원을 이용한 자원식물개발과 품종육성의 기초자료로 활용될 것으로 기대된다.

Abstract

RAPD (random amplified polymorphic DNA) analysis was examined to detect variation of in vitro cultured 30 rhizomes of Cymbidium goeringii Lindley and Cymbidium kanran Makino, with long-term (8 years) subculture, respectively. Out of 151 DNA bands detected, the 40 were polymorphic with a polymorphic rate 26.4% in the C. goeringii. Out of 155 DNA bands detected, the 56 were polymorphic with a polymorphic rate 36.1% in the C. kanran. Genetic similarity matrix (GSM) shows from 0.825 to 1.00 with an average of 0.944 in the rhizomes of C. goeringii and 0.812 to 1.00 with an average of 0.913 in the C. kanran. According to the clustering analysis, C. goeringii was divided into 1 group and 2 independent individuals and its structure of clustering was simple than that of C. kanran. The higher polymorphism and the decreased GSM were showed in the long-term in vitro cultured C. goeringii and C. kanran supplemented with growth regulators. The results provide as fundamental data to develop a new materials for plant breeding and resources plant.

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