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Genetic diversity and population structure of endangered Neofinetia falcata (Orchidaceae) in South Korea based on microsatellite analysis

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초록

Population genetic assessment is essential for the conservation and management of endangered and rare plants. Neofinetia falcata is endangered epiphyte orchid and protected by law in Korea. In Korea, this species is only found on islands in the South Sea of Korea (including Jeju-do) and the southern coast of the Korean Peninsula. We developed nine microsatellite makers to assess the genetic diversity and population genetic structure of three populations of N. falcata. The genetic diversity at the species level was low, which can be attributed to inbreeding or fragmentation into small, isolated populations. A recent bottleneck was detected in one population, likely due to overcollection. N. falcata exhibited moderated levels of differentiation among populations, with the three populations were divided into two clusters based on genetic structure. The genetic diversity and structure of N. falcata are affected by restricted gene flow by pollen or seeds due to isolation and geographic distance. Strategies for in situ and ex situ conservation of this species are been proposed based on the results of our study.

Abstract

Population genetic assessment is essential for the conservation and management of endangered and rare plants. Neofinetia falcata is endangered epiphyte orchid and protected by law in Korea. In Korea, this species is only found on islands in the South Sea of Korea (including Jeju-do) and the southern coast of the Korean Peninsula. We developed nine microsatellite makers to assess the genetic diversity and population genetic structure of three populations of N. falcata. The genetic diversity at the species level was low, which can be attributed to inbreeding or fragmentation into small, isolated populations. A recent bottleneck was detected in one population, likely due to overcollection. N. falcata exhibited moderated levels of differentiation among populations, with the three populations were divided into two clusters based on genetic structure. The genetic diversity and structure of N. falcata are affected by restricted gene flow by pollen or seeds due to isolation and geographic distance. Strategies for in situ and ex situ conservation of this species are been proposed based on the results of our study.

참고문헌 (60)

  1. Barman, D, "Climate change on orchid population and conservation strategies: A review", Journal of Crop and Weed, 9(2), pp.1~12 (2013)
  2. Chen, X, "Flora of China Orchidaceae (volume 25)", pp.483~484 (2009), Science Press, Beijing and Missouri Botanical Garden Press
  3. Chen, Y.-Y, "Genetic diversity and population structure of the medicinal orchid Gastrodia elata revealed by microsatellite analysis", Biochemical Systematics and Ecology, 54, pp.182~189 (2014)
  4. Chung, J.D, "Seed culture of Neofinetia falcata in vitro II. Effect of hyponex medium added with various concentrations of peptone or tryptone on asymbiotic germination of seeds and growth of seedlings", Journal of Plant Biotechnology, 7(1), pp.13~21 (1980)
  5. Chung, J.D, "Seed Culture of Neofinetia falcata in vitro Ⅲ. Effect of auxin, kinetin, vitamin and apple juice on growth of seedlings after transplanting", Journal of Plant Biotechnology, 8(1), pp.1~10 (1981)
  6. Cornuet, J.M, "Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data", Genetics, 144, pp.2001~2014 (1996)
  7. David, H.R, "Correlation between fitness and genetic diversity", Conservation Biology, 17(1), pp.230~237 (2003)
  8. Dressler, R.L, "Phylogeny and classification of the orchid family", pp.205~207 (1993), Dioscorides Press
  9. Duttke, S, "Mutant flower morphologies in the wind orchid, a novel orchid Model species", Plant Physiology, 158(4), pp.1542~1547 (2012)
  10. Earl, D.A, "STRUCTURE HARVESTER:a website and program for visualizing STRUC -TURE output and implementing the Evanno method", Conservation Genetics Resources, 4, pp.359~361 (2012)
  11. Ellstrand, N.C, "Population genetic consequences of small population size: Implications for plant conservation", Annual Review of Ecology and Systematics, 24, pp.217~242 (1993)
  12. Escudero, A, "Spatial analysis of genetic diversity as a tool for plant conservation", Biological Conservation, 113, pp.351~365 (2003)
  13. Evanno, G, "Detecting the number of clusters of individuals using the software STRUCTURE:A simulation study", Molecular Ecology, 14, pp.2611~2620 (2005)
  14. Excoffier, L, "Arlequin suite ver 3.5:A new series of programs to perform population genetics analyses under Linux and Windows", Molecular Ecology Resources, 10(3), pp.564~567 (2010)
  15. Frankham, R, "Relationship of genetic variation to population size in wildlife", Conservation Biology, 10(6), pp.1500~1508 (1996)
  16. Hahn, E.-J, "High photosynthetic photon flux and high CO 2 concentration under increased number of air exchanges promote growth and photosynthesis of four kinds of orchid plantlets in vitro", In Vitro Cellular & Development Biology-Plant, 37(5), pp.678~682 (2001)
  17. 한경숙, "Sclerotium rolfsii에 의한 소엽풍란 흰비단병", 식물병 연구, 16(3), pp.320~322 (2010), 한국식물병리학회
  18. 한경숙, "Botrytis cinerea에 의한 소엽풍란 잿빛곰팡이병", 한국균학회지, 41(4), pp.292~294 (2013), 한국균학회
  19. Hidayat, T, "Phylogeny of subtribe Aeridinae (Orchidaceae) inferred from DNA sequences data: Advanced analyses including Australasian genera", Jurnal Teknologi (Sciences & Engineering), 59(1suppl), pp.87~95 (2012)
  20. Honnay, O, "Susceptibility of common and rare plant species to the genetic consequences of habitat fragmentation", Conservation Biology, 21(3), pp.823~831 (2007)
  21. Hou, B, "Genetic diversity assessment and ex situ conservation strategy of the endangered Dendrobium officinale (Orchidaceae)using new trinucleotide microsatellite markers", Plant Systematics and Evolution, 298(8), pp.1483~1491 (2012)
  22. Hundera, K, "Both forest fragmentation and coffee cultivation negatively affect epiphytic orchid diversity in Ethiopian moist evergreen Afromontane forests", Biological Conservation, 159, pp.285~291 (2013)
  23. Ichihashi, S, "Effects of complex organic additives on callus growth in three orchid genera, Phalaenopsis, Doritaenopsis, and Neofinetia", Journal of the Japanese Society for Horticultural Science, 68(2), pp.269~274 (1999)
  24. Jakobsson, M, "CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure, version 1.1", Bioinformatics, 23, pp.1801~1806 (2007)
  25. Johansen, B.B, "Ex situ conservations of orchids", Opera Botanica, 113, pp.43~48 (1992)
  26. 김영기, "엽록체 matK 와 핵 ITS 염기서열을 이용한 나도풍란속 및 풍란속의 계통과 종동정", 식물분류학회지, 44(1), pp.39~50 (2014), 한국식물분류학회
  27. Kiyohara, S, "Characteristics of the falling speed of Japanese orchid seeds", International Journal of Biology, 4(3), pp.10~12 (2012)
  28. Kwak, M, "Genetic assessment of Abies koreana (Pinaceae), the endangered Korean fir, and conservation implications", Conservation genetics, 18(5), pp.1165~1176 (2017)
  29. Lavor, P, "Population genetics of the endemic and endangered Vriesea minarum (Bromeliaceae) in the Iron Quadrangle, Espinhaco range, Brazil", American Journal of Botany, 101(7), pp.1167~1175 (2014)
  30. Lee, N.S, "Illustrated Flora of Korean Orchids", pp.326~329 (2011), Ewha Womans University Press
  31. Leimu, R, "How general are positive relationships between plant population size, fitness and genetic variation?", Journal ofEcology, 94(5), pp.942~952 (2006)
  32. Mallet, B, "Evidence for isolation-by-habitat among populations of an epiphytic orchid species on a small oceanic island", PLOS ONE, 9(2), pp.e87469~ (2014)
  33. Miao, Y.C, "Phylogeography and genetic effects of habitat fragmentation on endangered Taxus yunnanensis in southwest China as revealed by microsatellite data", Plant Biology, 16, pp.365~374 (2014)
  34. Miao, Y.-C, "Microsatellite markers indicate genetic differences between cultivated and natural population of endangered Taxus yunnanensis", Botanical Journal of the Linnean Society, 177, pp.450~461 (2015)
  35. Michael, F.F, "Orchid conservation: further links", Annals of Botany, 118, pp.89~91 (2016)
  36. Mitsukuri, K, "Effects of explant source and dark-preconditioning on adventitious bud formation in Neofinetia falcata H. H. Hu in vitro", Journal of Japanese Society for Horticultural Science, 78(2), pp.252~256 (2009)
  37. Murren, C.J, "Effect of habitat fragmentation on pollination:pollinators, pollinia viability and reproductive success", Journal of Ecology, 20, pp.100~107 (2002)
  38. National Institute of Biological Resources, "Red data book of endangered vascular plants in Korea" (2012), National Institute of Biological Resources
  39. Peakall, R, "GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research – an update", Bioinformatics, 28, pp.2537~2539 (2012)
  40. Piry, S, "BOTTLENECK: a computer program for detecting recent reductions in the effective size using allele frequency data", The Journal of Heredity, 90(4), pp.502~503 (1999)
  41. Pritchard, J.K, "Inference of population structure using multilocus genotype data", Genetics, 155, pp.945~959 (2000)
  42. Rosenberg, N.A, "DISTRUCT: a program for the graphical display of population structure, version 1.1", Molecular Ecology Notes, 4, pp.137~138 (2004)
  43. Rozen, S, "Bioinformatics Methods and Protocols: Methods in molecular biologyTM vol. 132", pp.365~386 (2000), Humana Press
  44. Shanker, A, "Bioinformatically mined simple sequence repeats in UniGene of Citrus sinensis", Scientia Horticulturae, 113(4), pp.353~361 (2007)
  45. Shimizu, N, "Adaptation and evolution of seed shape on bleeding area in Japanese orchids", International journal of Biology, 4(2), pp.47~53 (2012)
  46. Shin, H, "Conservation biology of the Korea - the present situation and problem", pp.81~104 (2009), Worldscience Press
  47. Segelbacher, G, "From connectivity to isolation: genetic consequences of population fragmentation in capercaillie across Europe", Molecular Ecology, 12, pp.1773~1780 (2003)
  48. Spencer, C.C, "Experimental evaluation of the usefulness of microsatellite DNA for detecting demographic bottlenecks", Molecular Ecology, 9(10), pp.1517~1528 (2000)
  49. Su, Z, "Genetic diversity and structure of an endangered desert shrub and the implications for conservation", AoB Plants, 9(3), pp.plx016~ (2017)[DOI]
  50. Suetsugu, K, "Potential pollinator of Vanda falcata (Orchidaceae): Theretra (Lepidoptera: Sphingidae) hawkmoths are visitors of long spurred orchid", European Journal of Entomology, 112(2), pp.393~397 (2015)
  51. Sun, Y, "Genetic diversity and differentiation of Michelia maudiae (Magnoliaceae) revealed by nuclear and chlorophlast microsatellite markers", Genetica, 139, pp.1439~1447 (2011)
  52. Szpiech, Z.A, "ADZE: a rarefaction approach for counting alleles private to combinations of populations", Genetics and population analysis, 24(21), pp.2498~2504 (2008)
  53. Tian, H.Z, "Genetic diversity in the endangered terrestrial orchid Cypripedium japonicum in East Asia: Insights into population history and implications for conservation", Scienticific Reports, 8, pp.6467~ (2018)
  54. Trapnell, D.W, "Characterization of microsatellite loci for an australian epiphytic orchid, Dendrobium calamiforme, using illumina sequencing", Applications in Plant Sciences, 3(6), pp.1500016~ (2015)
  55. Weremijewicz, J, "Microsatellite primers for two threatened orchids in Florida: Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae)", Applications in Plant Sciences, 4(4), pp.1500095~ (2016)
  56. Wone, B.W.M, "Flight duration and flight muscle ultrastructure of unfed hawk moths", Arthropod Structure & Development (2018)
  57. Xu, Y, "Development and characterization of polymorphic ESTSSR markers for Paphiopedilum henryanum (Orchidace ae)", Applications in Plant Sciences, 6(5), pp.e1152~ (2018)
  58. Yoon, K.E, "Orchids of wold", pp.275~ (2011), Gimmyoung Press
  59. 윤영은, "Microsatellite 개발 및 분석법에 대한 소개", 식물분류학회지, 41(4), pp.299~314 (2011), 한국식물분류학회
  60. Zhang, X, "Inferring genetic variation and demographic history of Michelia yunnanensis Franch. (Magnoliaceae) from chloroplast DNA sequences and microsatellite markers", Frontiers in Plant Science, 8, pp.583~ (2017)

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