약용식물 수피 추출물이 반추위 발효와 미생물 성장 및 메탄 발생에 미치는 영향
Effects of Medicinal Plant Bark Extract on Rumen Fermentation, Microbial Growth and Methane Emission
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본 연구는 약용실물 수피 추출물을 이용하여 in vitro 반추위 발효성상 및 반추위 메탄 발생에 미치는 영향을 알아보기 위해 수행되었다. 공시재료는 무궁화, 음나무, 팽나무, 두충 및 후박나무 추출물을사용하였다. McDougall’s buffer와 반추위액을 2:1 비율로 혼합한 발효액을 티모시 0.3g과 식물 추출물이 담긴 50mL serum bottle에 혐기상태로 20mL 분주한 뒤, 39℃에서 3, 6, 9, 12, 24, 48 및 72시간 동안 발효하였다. 약용식물 수피 추출물을 첨가하였을 때 반추위 발효 성상(pH, 건물소화율, 암모니아태 질소, 미생물 성장량)에는 영향을 미치지 않았다. 배양 6시간대에 두충 추출물 첨가구에서acetate의 농도가 대조구에 비해 유의적(p<0.05)으로 낮고, 메탄의 기질인 이산화탄소의 발생량이 대조구에 비해 유의적(p<0.05)으로 낮아 메탄 저감 효과를 기대하였으나 메탄 저감효과는 없었다. 메탄생성박테리아인 Methanogen archaea의 경우 무궁화 추출물 첨가구를 제외한 전 추출물 첨가구에서 대조구에 비해 유의적(p<0.05)으로 높았으나 메탄발생량에는 유의적(p<0.05)인 차이가 없었다. 추후 첨가 농도를 달리하거나 flavonoid 함량을 측정하는 실험이 필요한 것으로 사료되며, 메탄생성박테리아를 감소시키는 연구도 추가적으로 필요할 것으로 생각된다.
Abstract
This study was conducted to evaluate the effects of bark extracts of medicinal herb on rumenfermentation, microbial growth and methane emission. The extracts of bark extracts of medicinalherb, as rose of sharon, castor aralia, chinese hackberry, hardy rubber tree and the silvermagnolia were used in this study. The 20 mL of mixture, comparing McDougall’s buffer andrumen fluid in the ratio 2 to 1, was dispensed anaerobically 50mL serum bottles containing0.3g of timothy substrate and extracts of bark extracts of medicinal herb. The serum bottleswere incubated 39℃ for 3, 6, 9, 12, 24, 48 and 72 hours. Supplementation of the bark extractsof medicinal herb extracts did not affect the fermentation characteristics(pH, dry matterdigestibility, ammonia concentration, rumen microbial growth), but not effect of reducing themethane. Acetate concentration was lower(p<0.05) for hardy rubber tree compared with control. Carbon dioxide production as a substrate for methane production in the rumen was lower (p<0.05) for hardy rubber tree compared with control whereas methane reduction effect did notexist. Methanogenic archaea diversity decreased in the rose of Sharon extract treatmentcompared with control whereas methane production was no differences between treatments. Therefore, further detail study may need to investigate of dose level dependence of the extracts,considering the flavonoid concentration of the extracts on the reduction of methane productionand methanogenic archaea.
참고문헌 (49)
- AOAC, "Official methods of analysis of AOAC INTERNATIONAL", AOAC, 1~44 (2012)
- Armentano LE, "Lack of response to degradable protein to a low protein diet fed to midlactation dirly cows", J. Dairy Sci, 76, pp.3755~3762 (1993)
- Broudiscou LP, "Effects of dry plant extracts on fermentation and methanogenesis in continuous culture of rumen microbes", Anim. Feed. Sci. Technol, 87, pp.263~277 (2000)
- Bodas R, "In vitro screening of the potential of numerous plant species as antimethanogenic feed additives for ruminants", Anim. Feed. Sci. Technol, 145, pp.245~258 (2008)
- Becker PM, "Evidence for a hydrogen-sink mechanism of (+)catechin-mediated emission reduction of the ruminant greenhouse gas methane", Metabolomics, pp.1~11 (2014)
- Cheeke PR., "Toxicity and metabolism of pyrrolizidine alkaloids", J. Anim. Sci, 66, pp.2343~2350 (1988)
- Chaney AL, "Modified reagents for determination of urea and ammonia", Clinical chemistry, 8, pp.130~132 (1962)
- Cushnie TPT, "Antimicrobial activity of flavonoids", Int. J. Antimicrob. Agents, 26, pp.343~356 (2005)
- Denman SE, "Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen", FEMS Microbiol. Ecol, 58, pp.572~582 (2006)
- Denman SE, "Quantitation and diversity analysis of ruminal methanogenic populations in reponse to the antimethanogenic compound bromochloromethane", FEMS Microbiol. Ecol, 62, pp.313~322 (2007)
- "Food and drug administration of the US, 21 CFR 184" (2004)[URL]
- Geol G, "Inhibition of methanogens by bromochloromethane: effects on microbial communities and rumen fermentation using batch and continuos fermentations", Br. J. Nutr, 101, pp.1484~1492 (2009)
- Ha JK, "Ruminant nutrition and physiology" (2013), Seoul National University press
- 황중덕, "음나무(Kalopanax pictus) 수피 추출물의 항산화 활성에 관한 연구", 대한미용학회지, 7(4), pp.329~337 (2011), 대한미용학회
- 장아람, "무궁화나무(Hibiscus syriacus) 수피 추출물의 페놀산 조성 및 기능성화장품 소재 가능성 평가", 한국미용학회지, 21(5), pp.895~902 (2015), 한국미용학회
- Jang JK., "Nexus BOOKS" (2009), Good native grass for the body
- Johnson EE, "Energy metabolism of farm animals", pp.376~379 (1991), ETH
- Johnson KA, "Methane emissions from cattle", J. Anim. Sci, 73, pp.2483~2492 (1995)
- Jouany JP, "Use of 'natural' products as alternatives to antibiotic feed additives in Ruminant production", Animal, 1, pp.1443~1466 (2007)
- Kazuo I, "Obovatol and obovatal, novel biphenyl ether kignans from the leaves of Magnolia obovata THUNB", Chem. Pharm. Bull, 30, pp.3347~3353 (1982)
- 김병기, "한방제재 첨가급여가 한우송아지의 성장, 혈액성상 및 설사에 미치는 영향", 한국축산학회지, 53(5), pp.451~459 (2011), 한국축산학회
- 김재황, "두충의 첨가급여가 거세한우의 생산성, 도체특성 및 육의 지방산 조성에 미치는 영향", 한국축산학회지, 47(6), pp.963~974 (2005), 한국축산학회
- Identification of flavonoids in useful arbor, Celtis sinensis(UImaceae), "Ko JM", The Korean Society of Plant Tissue Culture, 160~ (2007)
- Koike S, "Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus favefaciens", FEMS Microbiol. Lett, 204, pp.361~366 (2001)
- Lee CH, "Variation of kalosaponin contents in plant parts and population of Native Kalopanax septemlobus (Thunb.) Koidz", Kor. J. Pharmacogn, 31, pp.203~208 (2000)
- Lee DH., "Ebeecomm. We find wood easily with the five senses" (2012)
- 이강연, "겨자종자와 겨자분의 첨가가 반추위 발효성상과 메탄생성에 미치는 영향", 한국축산학회지, 55(1), pp.25~32 (2013), 한국축산학회
- Lee YJ, "Therapeutic applications of compounds in the Magnolia family", Pharmacol Ther, 130, pp.157~176 (2011)
- Maeng WJ., "Ruminant nutrition", pp.21~ (1998), Hyangmunsa
- Makkar HP, "Saponins used in food and agriculture", pp.387~394 (1996), Springer US
- Mitsubashi S, "On the drug resistance of enteric bacteria. 6. Spontaneous and artificial elimination of transferable drug resistance factors", Japan. J. Microbiol, 31, pp.119~123 (1961)
- Oskoueian E, "Effects of flavonoids on rumen fermentation activity, methane production, and microbial population", BioMed Research International, pp.8~ (2013)[DOI]
- Park JC, "Prevalence of anti-biotic-resistant strains among bacteria isolated from bovine mastitis, swine diarrhea, and swine pneumonia", Kor. J. Appl. Microbiol. Biotechnol, 28, pp.189~194 (2000)
- 박선애, "Eucommia ulmoides Oliver Leaf Extract Increases Endogenous Antioxidant Activity in Type 2 Diabetic Mice", Journal of Medicinal Food, 9(4), pp.474~479 (2006), 한국식품영양과학회
- Patra AK, "A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen", Phytochemistry, 71, pp.1198~1222 (2010)
- Pen B, "Effects of Yucca schidigera and Quillaja saponaria extracts on in vitro ruminal fermentation and methane emission", Anim. Feed. Sci. Tech, 129, pp.175~186 (2006)
- Rowe JB, "Changing rumen fermentation by chemical means", pp.102~ (1983), livestock library.com.au
- Skillman LC, "Development and validation of a real-time PCR method to quantify rumen protozoa and examination of variability between entodinium populations in sheep offered a hay-based diet", Appl. Environ. Microb, 72, pp.200~206 (2006)
- Stewart CS., "Factors affecting the cellulolytic activity of rumen contents", Appl. Environ. Microbiol, 33, pp.497~502 (1977)
- Tachikawa E, "Effects of extract and ingredients isolated from Magnolia obovata thunberg on catecholamine secretion from bovine adrenal chromaffin cells", Biochem. Pharmacol, 60, pp.433~440 (2000)
- Tamara F, "Use of Herbs and spices and their extracts in animal nutrition", Acta. Agric. Slov, 94, pp.95~102 (2009)
- Terry RA, "Effect of pH on cellulose digestion under in vitro conditions", J. Sci. Food Agric, 20, pp.317~320 (1969)
- Theodorou MK, "A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds", Anim. Feed Sci. Technol, 48, pp.185~197 (1994)
- Tyler SC, "Microbial Production and Consumption of Greenhouse Gases: Methane, Nitrogen Oxides, and Halomethane", pp.7~38 (1991), American Society of Microbiology
- Van Nevel CJ, "Influence of antibiotics and a deaminase inhibitor on volatile fatty acids and methane production from detergent washed hay and soluble starch by rumen microbes in vitro", Anim. Feed Sci. Technol, 37, pp.21~31 (1992)
- Van Soest PJ., "Nutritional ecology of the ruminant" (1994), Cornell University Press
- R. A. Afolayan, "Genetic Variation in Growth and Body Dimensions of Jersey and Limousin Cross Cattle. 1. Pre-Weaning Performance", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 15(10), pp.1371~1377 (2002), 아세아·태평양축산학회
- Wanapat M, "Development of feeding systems and strategies of supplementation to enhance rumen fermentation and ruminant production in the tropics", J. Anim. Sci. Biotechnol, 4, pp.32~ (2013)
- Caspar Wenk, "Herbs and Botanicals as Feed Additives in Monogastric Animals", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 16(2), pp.282~289 (2003), 아세아·태평양축산학회